AUTHOR=Sun Bing , Luo Huaizhi , Jiang Huan , Wang Zhennan , Jia Aiqun 

TITLE=Inhibition of Quorum Sensing and Biofilm Formation of Esculetin on Aeromonas Hydrophila

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.737626

DOI=10.3389/fmicb.2021.737626

ISSN=1664-302X

ABSTRACT=Quorum sensing (QS) and biofilm formation inhibition activity of esculetin on Aeromonas hydrophila SHAe 115 was evaluated. Exposure to esculetin at 25, 50, and 100 μg/mL significantly inhibited the production of protease and hemolysin, the formation of biofilms and attenuated the swarming motility of A. hydrophila SHAe 115. Biofilm forming inhibition was also observed through confocal laser scanning microscopy (CLSM) and scanning electron microscope (SEM). qRT-PCR analysis indicated that genes positively related to QS and biofilm formation were down-regulated to varying degrees, while gene (litR) negatively related to biofilm formation was significantly up-regulated. The phenotypic results were in good agreement with gene expression levels. These results indicated that esculetin would be a potential QS inhibitor for A. hydrophila.