AUTHOR=Jiang Weiwei , Hu Dongying , Xu Yanyan , Chen Yang , Zhu Xiaoyang , Han Zhao , Ye Xin , Li Xiaojing 

TITLE=Loop-mediated isothermal amplification-microfluidic chip for the detection of Trichophyton infection

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1031388

DOI=10.3389/fmicb.2022.1031388

ISSN=1664-302X

ABSTRACT=Trichophyton is the most pathogenic type of fungal skin infection. It often invades and grows in a keratin-rich matrix, and lesions include human skin, hair, and fingernails (toenails). We designed the LAMP primers of Trichophyton, and developed the LAMP- Microfluidic chip detection system of Trichophyton for the first time. It can detect 6 common species of Trichophyton in the genus Trichophyton, including Trichophyton rubrum, Trichophyton mentagrophyte, Trichophyton violaceum, Trichophyton tonsurans, Trichophyton verrucosum and Trichophyton schoenleinii. The sensitivity can reach 100%, and the sensitivity can reach about 1×102 copies/μL. The entire detection process can be completed quickly within 60 minutes and does not cross-react with other Trichophyton species. The established LAMP- Microfluidic chip detection system has the advantages of simple operation, high specificity and high sensitivity, and has potential for clinical application.