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<journal-id journal-id-type="publisher-id">Front. Microbiol.</journal-id>
<journal-title>Frontiers in Microbiology</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Microbiol.</abbrev-journal-title>
<issn pub-type="epub">1664-302X</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
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<article-meta>
<article-id pub-id-type="doi">10.3389/fmicb.2022.1081051</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Microbiology</subject>
<subj-group>
<subject>Correction</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Corrigendum: Iron deprivation enhances transcriptional responses to <italic>in vitro</italic> growth arrest of <italic>Mycobacterium tuberculosis</italic></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name><surname>Alebouyeh</surname> <given-names>Sogol</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="author-notes" rid="fn002"><sup>&#x02020;</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/2012888/overview"/>
</contrib>
<contrib contrib-type="author">
<name><surname>C&#x000E1;rdenas-Pestana</surname> <given-names>Jorge A.</given-names></name>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref>
<xref ref-type="aff" rid="aff3"><sup>3</sup></xref>
<xref ref-type="author-notes" rid="fn002"><sup>&#x02020;</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/1582311/overview"/>
</contrib>
<contrib contrib-type="author">
<name><surname>Vazquez</surname> <given-names>Lucia</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/2013533/overview"/>
</contrib>
<contrib contrib-type="author">
<name><surname>Prados-Rosales</surname> <given-names>Rafael</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/1579216/overview"/>
</contrib>
<contrib contrib-type="author">
<name><surname>Del Portillo</surname> <given-names>Patricia</given-names></name>
<xref ref-type="aff" rid="aff4"><sup>4</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/601610/overview"/>
</contrib>
<contrib contrib-type="author" corresp="yes">
<name><surname>Sanz</surname> <given-names>Joaqu&#x000ED;n</given-names></name>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref>
<xref ref-type="aff" rid="aff3"><sup>3</sup></xref>
<xref ref-type="corresp" rid="c003"><sup>&#x0002A;</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/1494065/overview"/>
</contrib>
<contrib contrib-type="author" corresp="yes">
<name><surname>Men&#x000E9;ndez</surname> <given-names>Maria Carmen</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="corresp" rid="c002"><sup>&#x0002A;</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/387423/overview"/>
</contrib>
<contrib contrib-type="author" corresp="yes">
<name><surname>Garc&#x000ED;a</surname> <given-names>Maria J.</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="corresp" rid="c001"><sup>&#x0002A;</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/387382/overview"/>
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<aff id="aff1"><sup>1</sup><institution>Department of Preventive Medicine and Public Health and Microbiology, School of Medicine, Autonomous University of Madrid</institution>, <addr-line>Madrid</addr-line>, <country>Spain</country></aff>
<aff id="aff2"><sup>2</sup><institution>Department of Theoretical Physics, University of Zaragoza</institution>, <addr-line>Zaragoza</addr-line>, <country>Spain</country></aff>
<aff id="aff3"><sup>3</sup><institution>Institute for Biocomputation and Physics of Complex Systems (BIFI), University of Zaragoza</institution>, <addr-line>Zaragoza</addr-line>, <country>Spain</country></aff>
<aff id="aff4"><sup>4</sup><institution>Corporaci&#x000F3;n CorpoGen</institution>, <addr-line>Bogota</addr-line>, <country>Colombia</country></aff>
<author-notes>
<fn fn-type="edited-by"><p>Edited and reviewed by: Nuno S. Os&#x000F3;rio, ICVS/3B&#x00027;s Associate Laboratory (AL), Portugal</p></fn>
<corresp id="c001">&#x0002A;Correspondence: Maria J. Garc&#x000ED;a <email>mariaj.garcia&#x00040;uam.es</email></corresp>
<corresp id="c002">Maria Carmen Men&#x000E9;ndez <email>carmen.menendez&#x00040;uam.es</email></corresp>
<corresp id="c003">Joaqu&#x000ED;n Sanz <email>jsanz&#x00040;bifi.es</email></corresp>
<fn fn-type="other" id="fn001"><p>This article was submitted to Evolutionary and Genomic Microbiology, a section of the journal Frontiers in Microbiology</p></fn>
<fn fn-type="equal" id="fn002"><p>&#x02020;These authors have contributed equally to this work and share first authorship</p></fn></author-notes>
<pub-date pub-type="epub">
<day>29</day>
<month>11</month>
<year>2022</year>
</pub-date>
<pub-date pub-type="collection">
<year>2022</year>
</pub-date>
<volume>13</volume>
<elocation-id>1081051</elocation-id>
<history>
<date date-type="received">
<day>26</day>
<month>10</month>
<year>2022</year>
</date>
<date date-type="accepted">
<day>27</day>
<month>10</month>
<year>2022</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#x000A9; 2022 Alebouyeh, C&#x000E1;rdenas-Pestana, Vazquez, Prados-Rosales, Del Portillo, Sanz, Men&#x000E9;ndez and Garc&#x000ED;a.</copyright-statement>
<copyright-year>2022</copyright-year>
<copyright-holder>Alebouyeh, C&#x000E1;rdenas-Pestana, Vazquez, Prados-Rosales, Del Portillo, Sanz, Men&#x000E9;ndez and Garc&#x000ED;a</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/"><p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p></license>
</permissions>
<related-article id="RA1" related-article-type="corrected-article" journal-id="Front. Microbiol." journal-id-type="nlm-ta" vol="13" page="956602" xlink:href="10.3389/fmicb.2022.956602" ext-link-type="doi">A corrigendum on <article-title>Iron deprivation enhances transcriptional responses to <italic>in vitro</italic> growth arrest of <italic>Mycobacterium tuberculosis</italic></article-title> by Alebouyeh, S., C&#x000E1;rdenas-Pestana, J. A., Vazquez, L., Prados-Rosales, R., Del Portillo, P., Sanz, J., Men&#x000E9;ndez, M. C., and Garc&#x000ED;a, M. J. (2022). <italic>Front. Microbiol</italic>. 13:956602. doi: <object-id>10.3389/fmicb.2022.956602</object-id></related-article>
<kwd-group>
<kwd><italic>Mycobacterium tuberculosis</italic></kwd>
<kwd>iron availability</kwd>
<kwd>transcriptomics</kwd>
<kwd>growth arrest</kwd>
<kwd>metabolic changes</kwd>
</kwd-group>
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<fig-count count="1"/>
<table-count count="0"/>
<equation-count count="0"/>
<ref-count count="1"/>
<page-count count="3"/>
<word-count count="1133"/>
</counts>
</article-meta>
</front>
<body>
<p>In the published article, there was an error in the legend of <xref ref-type="fig" rid="F7">Figure 7B</xref> as published.</p>
<p>Analysis of lipid content by thin layer chromatography. <bold>(A)</bold> Mycolic acids. FAME, fatty acids methyl esters. <bold>(B)</bold> Polar lipids: PGL, phenolic glycolipid; GPL, glycopeptidolipids; TMM, trehalose monomycolate; PIMs, phosphatidyl-inositol mannosides; Cl, chloroform; Met, methanol; W, water. <bold>(C)</bold> Apolar lipids: PDIM, phthiocerol dymycocerosate; TAG, triacylglycerol; MQs, menaquinones; PE, petroleum ether; DE, diethyl ether. Lanes: a, Exp5&#x02013;Fe; b, Exp5&#x0002B;Fe; c, Stat6&#x02013;Fe; and d, Stat6&#x0002B;Fe.</p>
<p>The corrected legend appears below.</p>
<p>In the published article, there was an error in <xref ref-type="fig" rid="F7">Figure 7B</xref> as published. Names of lipids are wrongly label.</p>
<p>The corrected <xref ref-type="fig" rid="F7">Figure 7</xref> and its caption appear below.</p>
<fig id="F7" position="float">
<label>Figure 7</label>
<caption><p>Analysis of lipid content by thin layer chromatography. <bold>(A)</bold> Mycolic acids. FAME, fatty acids methyl esters. <bold>(B)</bold> Polar lipids: TDM, trehalose dimycolate; TMM, trehalose monomycolate; Cl, chloroform; Met, methanol; W, water. <bold>(C)</bold> Apolar lipids: PDIM, phthiocerol dymycocerosate; TAG, triacylglycerol; MQs, menaquinones; PE, petroleum ether; DE, diethyl ether. Lanes: a, Exp5&#x02013;Fe; b, Exp5&#x0002B;Fe; c, Stat6&#x02013;Fe; and d, Stat6&#x0002B;Fe.</p></caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmicb-13-1081051-g0001.tif"/>
</fig>
<p>In the published article, there was an error. Page 10, <bold>Results</bold>.</p>
<p>A correction has been made to section <bold>Results</bold>, subsection <bold>Lipids characterization</bold>. This sentence previously stated:</p>
<p>&#x0201C;To gain insight into lipid changes linked to the effect of iron and growth arrest, TLC analysis was performed on whole Mtb cells submitted to the four different conditions under study: Exp5 and Stat6, any of them; with (&#x0002B;Fe) and without iron (&#x02212;Fe). No differences were detected in the mycolic acid composition of the bacteria under the different conditions used (<xref ref-type="fig" rid="F7">Figure 7A</xref>). Concerning total lipid analysis, conditions to develop polar and non-polar lipids were applied (<xref ref-type="fig" rid="F7">Figures 7B</xref>,<xref ref-type="fig" rid="F7">C</xref>). The analysis of polar lipids showed a higher abundance of PIMs and glycopeptidolipids (GPL) at Stat6 phase compared to Exp5 phase (<xref ref-type="fig" rid="F7">Figure 7B</xref>). The opposite result was observed when apolar lipids were analyzed (<xref ref-type="fig" rid="F7">Figure 7C</xref>). Further characterization to confirm the detection of PIMs in Exp5 phase, was performed by using two-dimensional TLC (Supplementary Figure 6). Interestingly, by applying conditions aimed at resolving apolar lipids, we observed that the band corresponding to PDIM was visible at Exp5 phase but was not detected at Stat6 phase independently of the iron content (<xref ref-type="fig" rid="F7">Figure 7C</xref>). Similar to previous data (Bacon et al., <xref ref-type="bibr" rid="B1">2007</xref>) increased levels of MQs were detected in iron starvation during exponential phase (<xref ref-type="fig" rid="F7">Figure 7C</xref>). We also detected increased levels of TAG in stationary phase, in agreement with the detected higher proportion of red-nile stained bacilli (Figure 2).&#x0201D;</p>
<p>The corrected sentence appears below:</p>
<p>&#x0201C;To gain insight into lipid changes linked to the effect of iron and growth arrest, TLC analysis was performed on whole Mtb cells submitted to the four different conditions under study: Exp5 and Stat6, any of them; with (&#x0002B;Fe) and without iron (-Fe). No differences were detected in the mycolic acid composition of the bacteria under the different conditions used (<xref ref-type="fig" rid="F7">Figure 7A</xref>). Concerning total lipid analysis, conditions to develop polar and non-polar lipids were applied (<xref ref-type="fig" rid="F7">Figures 7B</xref>,<xref ref-type="fig" rid="F7">C</xref>). The analysis of polar lipids showed a higher abundance of trehalose mono- (TMM) and dimycolates (TDM) at Stat6 phase compared to Exp5 phase (<xref ref-type="fig" rid="F7">Figure 7B</xref>). The opposite result was observed when apolar lipids were analyzed (<xref ref-type="fig" rid="F7">Figure 7C</xref>). Further characterization to confirm the detection of PIMs in Exp5 phase, was performed by using two-dimensional TLC (Supplementary Figure 6). Interestingly, by applying conditions aimed at resolving apolar lipids, we observed that the band corresponding to PDIM was visible at Exp5 phase but was not detected at Stat6 phase independently of the iron content (<xref ref-type="fig" rid="F7">Figure 7C</xref>). Similar to previous data (Bacon et al., <xref ref-type="bibr" rid="B1">2007</xref>) increased levels of MQs were detected in iron starvation during exponential phase (<xref ref-type="fig" rid="F7">Figure 7C</xref>). We also detected increased levels of TAG in stationary phase, in agreement with the detected higher proportion of red-nile stained bacilli (Figure 2).&#x0201D;</p>
<p>The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.</p>
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</body>
<back>
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<title>References</title>
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<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Bacon</surname> <given-names>J.</given-names></name> <name><surname>Dover</surname> <given-names>L. G.</given-names></name> <name><surname>Hatch</surname> <given-names>K. A.</given-names></name> <name><surname>Zhang</surname> <given-names>Y.</given-names></name> <name><surname>Gomes</surname> <given-names>J. M.</given-names></name> <name><surname>Kendall</surname> <given-names>S.</given-names></name> <etal/></person-group>. (<year>2007</year>). <article-title>Lipid composition and transcriptional response of <italic>Mycobacterium tuberculosis</italic> grown under iron-limitation in continuous culture: identification of a novel wax ester</article-title>. <source>Microbiology</source> <volume>153</volume> (<issue>Pt. 5</issue>), <fpage>1435</fpage>&#x02013;<lpage>1444</lpage>. <pub-id pub-id-type="doi">10.1099/mic.0.2006/004317-0</pub-id><pub-id pub-id-type="pmid">17464057</pub-id></citation></ref>
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</article>