AUTHOR=Wang Haijie , Cao Limin , Li Qi , Wijayawardene Nalin N. , Zhao Jian , Cheng Min , Li Qi-Rui , Li Xiaobin , Promputtha Itthayakorn , Kang Ying-Qian 

TITLE=Overexpressing GRE3 in Saccharomyces cerevisiae enables high ethanol production from different lignocellulose hydrolysates

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1085114

DOI=10.3389/fmicb.2022.1085114

ISSN=1664-302X

ABSTRACT=Genetically modified strains for efficient use of xylose and developing lignocellulosic hydrolysates play an essential role in facilitating cellulosic ethanol production. Here we present a promising strain GRE3OE via GRE3 overexpressed in a previously reported Saccharomyces cerevisiae strain WXY70. A comprehensive evaluation of the fermentation level of GRE3OE in alkaline-distilled sweet sorghum bagasse, sorghum straw and xylose mother liquor hydrolysate. Under simulated corn stover hydrolysate, GRE3OE produced 53.39 g/L ethanol within 48 h. GRE3OE produced about 0.498 g/g total sugar in sorghum straw hydrolysate solution. Moreover, GRE3OE consumed more xylose than WXY70 in the high-concentration xylose mother liquor. Taken together, GRE3OE achieved remarkable fermentation efficiencies in three kinds of hydrolysates solution, which outperformed the previous strain WXY70.