AUTHOR=Sinha Sweta , Das Souvik , Saha Biswajit , Paul Debarati , Basu Biswarup 

TITLE=Anti-microbial, anti-oxidant, and anti-breast cancer properties unraveled in yeast carotenoids produced via cost-effective fermentation technique utilizing waste hydrolysate

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 13 - 2022

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1088477

DOI=10.3389/fmicb.2022.1088477

ISSN=1664-302X

ABSTRACT=Vegetable “mandi” waste was used to prepare waste hydrolysate, a culture medium, in which oleaginous red yeast Rhodosporidium sp. was grown. Carotenoid pigments were extracted using solvent extraction method and analysed by UV spectroscopy, TLC and HPLC. Antimicrobial, antioxidant and anticancer activities of the extract were evaluated, followed by in silico docking and ADME/T studies. Carotenoids extract was found to be composed of three main pigments- β-carotene, torulene, and torularhodin. Extract exhibited significant antioxidant, antimicrobial and anti-breast cancer activities in vitro while being biocompatible.  In silico evaluation predicted binding with breast cancer specific molecular targets, good drug likeliness properties, and less toxicity. This is the first report on anti-breast cancer activities, particularly in triple negative breast cancer cells, by red yeast carotenoids produced via a sustainable environment friendly bioprocess utilising waste hydrolysate.