AUTHOR=Garcia-Gonzalez Natalia , Comas Joan Colom , Harris Hugh M. B. , Strain Conall , Stanton Catherine , Hill Colin , Corsetti Aldo , Gahan Cormac G. M. 

TITLE=Impact of Food Origin Lactiplantibacillus plantarum Strains on the Human Intestinal Microbiota in an in vitro System

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.832513

DOI=10.3389/fmicb.2022.832513

ISSN=1664-302X

ABSTRACT=Although the pathology of inflammatory bowel disease (IBD) is still unclear, many studies suggest a role for altered gut microbiota. In the current study we evaluated the potential impact of food-dominant Lactiplantibacillus (Lpb.) plantarum strains as a strategy to manipulate microbial diversity in complex microbiota’s derived from a healthy human and an IBD patient using a continuous fecal fermentation model. Our results showed that introduction of Lpb. plantarum strains into the community led to a significant increase in alpha diversity and a separation of control and treatment groups in beta diversity for the IBD sample. This was reflected in significant alterations in abundance of the unclassified genera, dominated by Enterobacteriaceae_unclass and Ruminococcaceae_unclass. However, the microbiota of the healthy individual was relatively unaffected following introduction of Lpb. plantarum strains. Overall, the work describes a novel application of the fecal fermentation model for the analysis of probiotic/food interactions in a disease state.