AUTHOR=Sun Pengfei , Huang Xiao , Xing Yixiao , Dong Wenlong , Yu Jianghua , Bai Jie , Duan Weiyan 

TITLE=Immobilization of Ochrobactrum sp. on Biochar/Clay Composite Particle: Optimization of Preparation and Performance for Nitrogen Removal

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.838836

DOI=10.3389/fmicb.2022.838836

ISSN=1664-302X

ABSTRACT=<p>The objective of this study was to prepare biochar/clay composite particle (BCCP) as carrier to immobilize <italic>Ochrobactrum</italic> sp. to degrade ammonia nitrogen (NH<sub>4</sub><sup>+</sup>-N), and the effects of calcined program and immobilizing material were investigated. Results reflected that the parameters were as follows: calcined temperature 400°C, heating rate 20°C min<sup>–1</sup>, and holding time 2 h, and the adsorption capacity could reach 0.492 mg g<sup>–1</sup>. Sodium alginate/polyvinyl alcohol, as embedding material, jointed with NH<sub>4</sub><sup>+</sup>-N adsorption process and then degraded by <italic>Ochrobactrum</italic> sp. with 79.39% degradation efficiency at 168 h. Immobilizing <italic>Ochrobactrum</italic> sp. could protect strain from high salt concentration to achieve the exceeding degradation efficiency than free bacteria, but could not block the impact of low temperature.</p>