AUTHOR=Khanongnuch Ramita , Mangayil Rahul , Santala Ville , Hestnes Anne Grethe , Svenning Mette Marianne , Rissanen Antti J. 

TITLE=Batch Experiments Demonstrating a Two-Stage Bacterial Process Coupling Methanotrophic and Heterotrophic Bacteria for 1-Alkene Production From Methane

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.874627

DOI=10.3389/fmicb.2022.874627

ISSN=1664-302X

ABSTRACT=<p>Methane (CH<sub>4</sub>) is a sustainable carbon feedstock for value-added chemical production in aerobic CH<sub>4</sub>-oxidizing bacteria (methanotrophs). Under substrate-limited (e.g., oxygen and nitrogen) conditions, CH<sub>4</sub> oxidation results in the production of various short-chain organic acids and platform chemicals. These CH<sub>4</sub>-derived products could be broadened by utilizing them as feedstocks for heterotrophic bacteria. As a proof of concept, a two-stage system for CH<sub>4</sub> abatement and 1-alkene production was developed in this study. Type I and Type II methanotrophs, <italic>Methylobacter tundripaludum</italic> SV96 and <italic>Methylocystis rosea</italic> SV97, respectively, were investigated in batch tests under different CH<sub>4</sub> and air supplementation schemes. CH<sub>4</sub> oxidation under either microaerobic or aerobic conditions induced the production of formate, acetate, succinate, and malate in <italic>M. tundripaludum</italic> SV96, accounting for 4.8–7.0% of consumed carbon from CH<sub>4</sub> (C-CH<sub>4</sub>), while <italic>M. rosea</italic> SV97 produced the same compounds except for malate, and with lower efficiency than <italic>M. tundripaludum</italic> SV96, accounting for 0.7–1.8% of consumed C-CH<sub>4</sub>. For the first time, this study demonstrated the use of organic acid-rich spent media of methanotrophs cultivating engineered <italic>Acinetobacter baylyi</italic> ADP1 ‘<italic>tesA-undA</italic> cells for 1-alkene production. The highest yield of 1-undecene was obtained from the spent medium of <italic>M. tundripaludum</italic> SV96 at 68.9 ± 11.6 μmol mol C<sub>substrate</sub><sup>–1</sup>. However, further large-scale studies on fermenters and their optimization are required to increase the production yields of organic acids in methanotrophs.</p>