AUTHOR=Li Wansheng , Li Minhua , Zhang Hongliang , Li Chao , Xu Hu , Gong Bangjun , Fu Jun , Guo Zhenyang , Peng Jinmei , Zhou Guohui , Tian Zhijun , Wang Qian 

TITLE=A Novel Immunochromatographic Strip Based on Latex Microspheres for the Rapid Detection of North American-Type Porcine Reproductive and Respiratory Syndrome Virus

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.882112

DOI=10.3389/fmicb.2022.882112

ISSN=1664-302X

ABSTRACT=A latex microsphere-based immunochromatographic strip (ICS) test was successfully developed for the rapid and sensitive detection of porcine reproductive and respiratory syndrome virus (PRRSV). The PRRSV N protein-specific monoclonal antibody (mAb) 1H4 labeled with latex microspheres was dispensed on a conjugate pad for use as the detector. The same mAb and goat anti-mouse antibody were blotted on a nitrocellulose membrane to form test and control lines, respectively. The limit of virus detection was approximately 5×102.0 median tissue culture infectious dose (TCID50)/mL. The limit of N protein detection was approximately 15 ng/mL. Other common porcine viruses were tested to evaluate the specificity of the ICS, and positive results were observed for only North American-Type PRRSV. A comparison of the strip with a standard diagnostic test (reverse transcriptase polymerase chain reaction, RT-PCR) was also performed. The ICS test was shown to be of relative high specificity and sensitivity, 90.32% and 73.91%, respectively. These results suggest that the ICS test can be used to quickly and accurately detect PRRSV and can be suitable for diagnostic purposes in the field.