AUTHOR=Yang Yi , Qiu Jingxuan , Wang Xin 

TITLE=Exploring the Dynamic of Bacterial Communities in Manila Clam (Ruditapes philippinarum) During Refrigerated Storage

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.882629

DOI=10.3389/fmicb.2022.882629

ISSN=1664-302X

ABSTRACT=Microorganism contamination is one of the most important factors affecting the spoilage and food safety of Manila clams. This study aimed to gain insights into bacterial composition and the dynamic change of bacterial communities on retailed Manila clam during refrigerated storage within the edible period. High throughput sequencing was conducted to monitor the bacterial population with the prolongation of storage time of Day 0, 1 and 3. Result demonstrated that phyla of Proteobacteria, Actinobacteriota, Acidobacteriota, and Chloroflexi composed the majority of bacterial during the whole observation process. Furthermore, the increase of Proteobacteria showed a positive correlation with the storage time, while Acidobacteriota and Chloroflexi continued to decline in storage. For genus annotation, none of genus obtained dominant population in storage. From Day 0 to Day 1, the genera of Streptomyces, Bradyrhizobium, and Mycobacterium significantly increased, meanwhile, 12 genera got a significant decrease. Compared with samples in Day 0, a total of 15 genera significantly decreased with the reduced proportion ranging from 0.50% to 4.40% in Day 3. At the end of the storage, the genera of Crossiella become the most redundant population. Both the richness and diversity got a decrease at the start of storage in Day 1, and then a slight increase in Day 3 was observed. Based on the result in this study, strategy targeting the increased bacteria could be tested to improve the consumption quality and safety of refrigerated clam.