AUTHOR=Zhao Teng , Li Bo-qi , Gao He-ting , Xing Dan , Li Man-jin , Dang Yun-qi , Zhang Heng-duan , Zhao Yue-e , Liu Zhu , Li Chun-xiao 

TITLE=Metagenome Sequencing Reveals the Microbiome of Aedes albopictus and Its Possible Relationship With Dengue Virus Susceptibility

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.891151

DOI=10.3389/fmicb.2022.891151

ISSN=1664-302X

ABSTRACT=Dengue fever virus (DENV) is a mosquito-borne flavivirus that poses a serious risk to human health. Aedes albopictus is a widely-distributed vector of dengue fever in China. Based the impact on physiological activity, the microbiome in Aedes albopictus will provide a novel environment-friendly approach to control DENV transmission. We performed metagenomic sequencing on Aedes albopictus before and after exposure to DENV blood meal to detect microbiome variation of Aedes albopictus with different susceptibilities to DENV. The dominant phyla in Aedes albopictus microbiome were Proteobacteria and Ascomycota, and the dominant genera were Aspergillus and Metarhizium. GammaProteobacteria bacterium,Lactobacillus harbinensis and Neurospora crassa differed significantly after DENV infection. There were 15 different microorganisms found to be involved in mosquito immunity and metabolism, including AlphaProteobacteria bacterium, Methyloglobulus morosus and Shigella sonnei, which might have an impact on the DENV susceptibility of Aedes albopictus. It was hypothesized that the lack of specific bacteria may lead to increased susceptibility of Aedes albopictus to DENV. Interventions in the microbiome composition or specific bacteria of Aedes albopictus may affect the susceptibility to DENV and control the mosquito-borne diseases efficiently.