AUTHOR=Li Zeyang , Zheng Yingying , Li Yansu , Cheng Xu , Huang Sanwen , Yang Xueyong , Qin Yuxuan 

TITLE=Genotype-Specific Recruitment of Rhizosphere Bacteria From Sandy Loam Soil for Growth Promotion of Cucumis sativus var. hardwickii

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.910644

DOI=10.3389/fmicb.2022.910644

ISSN=1664-302X

ABSTRACT=The composition and structure of rhizosphere microbiome is affected by many factors including soil type, genotype and cultivation time of plant, however, the interaction mechanisms among these factors are largely unclear. We use culture-independent 16S rRNA amplicon sequencing to investigate the rhizosphere bacterial composition and structure of cultivated cucumber Xintaimici (XT) and wild-type cucumber Cucumis sativus var. hardwickii (HD) in four kinds of soils. We found that soil type, cultivation time, and genotype all affected the composition and structure of cucumber rhizosphere bacterial communities. Notably, HD showed better physiological features in sandy soil and sandy loam soil than black soil and farm soil at 50 days post sowing, which due to its stronger recruitment ability to Nitrospira, Nocardioides, Bacillus and Gaiella in sandy soil, and more Tumebacillus, Nitrospira and Paenibacillus in sandy loam soil. Meanwhile, we also found that HD showed better recruiting capacity for these bacterial genera than XT in both sandy soil and sandy loam soil. Functional predictions indicated that these bacteria may had stronger root colonization ability and then promoted the growth of cucumber by enhancing the nitrogen metabolism and active metabolites secretion. In this study, our finding provided a better insight into the relationship among cucumber phenotype, genotype and rhizosphere bacterial community, which will offer valuable theoretical references for rhizosphere microbiota studies, and its future application in agriculture.