AUTHOR=Sawicki Rafał , Widelski Jarosław , Okińczyc Piotr , Truszkiewicz Wiesław , Glous Joanna , Sieniawska Elwira 

TITLE=Exposure to Nepalese Propolis Alters the Metabolic State of Mycobacterium tuberculosis

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.929476

DOI=10.3389/fmicb.2022.929476

ISSN=1664-302X

ABSTRACT=Propolis is a natural product proved to be efficient against Mycobacterium tuberculosis. Although it is produced by bees, its active alcoholic-aqueous fraction contains plant derived molecules. To gain some insight into its mechanism of antimycobacterial activity, we studied the metabolic changes in bacterial cells treated with extract of Trigona sp. propolis from Nepal. The early bacterial response was related to the increased demand for energy and upregulation of molecules involved in the formation of the cell membrane. Transcriptomic analysis confirmed that bacteria suffered also from oxidative stress, which was pronounced on the second day of exposure.