AUTHOR=Alam Khorshed , Hao Jinfang , Zhong Lin , Fan Guoqing , Ouyang Qing , Islam Md. Mahmudul , Islam Saiful , Sun Hongluan , Zhang Youming , Li Ruijuan , Li Aiying 

TITLE=Complete genome sequencing and in silico genome mining reveal the promising metabolic potential in Streptomyces strain CS-7

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.939919

DOI=10.3389/fmicb.2022.939919

ISSN=1664-302X

ABSTRACT=Gram-positive Streptomyces bacteria can produce valuable antibiotics and secondary metabolites. Streptomyces genomes include huge unknown silent natural product (NP) biosynthetic gene clusters (BGCs), making them a potential drug discovery repository. In order to collect antibiotic-producing bacteria from unexplored areas, we identified Streptomyces spp. CS-7 from mountain soil samples in Changsha, P.R. China, which showed strong antibacterial activity. Complete genome sequencing and prediction in silico revealed that its 8.4 Mbp genome contains a total of 36 BGCs for NPs. We purified two important antibiotics from this strain, which were structurally elucidated to be mayamycin and mayamycin B active against Staphylococcus aureus. The data here supported this Streptomyces species especially from unexplored habitats has a high potential for new NPs.