AUTHOR=Ma Yulin , Chen Xu , Khan Muhammad Zahoor , Xiao Jianxin , Cao Zhijun 

TITLE=A Combination of Novel Microecological Agents and Molasses Role in Digestibility and Fermentation of Rice Straw by Facilitating the Ruminal Microbial Colonization

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.948049

DOI=10.3389/fmicb.2022.948049

ISSN=1664-302X

ABSTRACT=In the current study, we evaluated the microecological agents (MA) combination with molasses (M) on the biodegradation of rice straw in the rumen. Rice straw was pretreated in laboratory polyethylene a 25 cm x 35 cm sterile bags with no additive control (Con), MA and MA + M for 7, 15, 30 and 45 days and then the efficacy of MA + M pretreatment was evaluated both in vitro and in vivo. The Scanning electron microscopy, X-ray diffraction analysis and Fourier-transform infrared spectroscopy, respectively results showed the MA or MA + M pretreatment altered the physical and chemical structure of rice straw. Meanwhile, the ruminal microbial attachment on the surface of rice straw was significantly increased after MA+M pretreated. Furthermore, the MA + M not only promoted rice straw fermentation in vitro but also improved digestibility by specifically inducing rumen colonization of Prevotellaceae_UCG-001, Butyrivibrio and Succinimonas. Altogether, we concluded that microecological agents and molasses could be the best choice as a biological pretreatment for rice straw to enhance their nutritive value as a ruminant’s feed.