AUTHOR=Zhang Fangfang , Xiao Ying , Pan Liang , Yu Ling , Liu Yanfang , Li Deshun , Liu Xiaojie 

TITLE=Effects of polysaccharides from Lyophyllum decastes (Fr.) Singer on gut microbiota via in vitro-simulated digestion and fermentation

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1083917

DOI=10.3389/fmicb.2023.1083917

ISSN=1664-302X

ABSTRACT=Lyophyllum decastes (Fr.) Singer polysaccharides (LDSPs) have been verified to possess strong biological properties. However, the effects of LDSPs on the intestinal microbes and their metabolites have rarely been addressed. Therefore, the in vitro simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study. The results showed a slight increase in content of reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion, indicating that digestion did not remarkedly affect the overall structure of LDSPs. After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and the pH of the fermentation solution decreased significantly (p < 0.05), suggesting that the LDSPs could be transformed into short chain fatty acids by human gut microbiota. Moreover, 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group. Notably, the LDSPs group directed a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides, and an increase in n-butyrate level. These findings suggest that LDSPs might be a potential prebiotic to provide a health benefit.