AUTHOR=Ailizire Ainiwaer , Wang Xiaojing , Ma Yan , Yan Xin , Li Shiqi , Wu Ziyi , Du Wenqi 

TITLE=How hypoxia affects microbiota metabolism in mice

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1244519

DOI=10.3389/fmicb.2023.1244519

ISSN=1664-302X

ABSTRACT=To investigate the relationship between gut microbiota and the faecal metabolise of hypoxic environments in mice. Methods: High-fat diet-induced obese mice (n=20) and normal diet-fed mice (n=20) were randomly divided into four groups: high altitude obese group (HOB), high altitude normal weight group (HN), low altitude obese group LOB (LOB) and low altitude normal weight group (LN). Faecal samples from each group were 16S rRNA gene sequenced, and five samples from each of the four groups above were selected for non-targeted faecal metabolomics analysis using liquid chromatography-mass spectrometry. The relationship between gut microbiota and faecal metabolites was analysed using SIMCA 14.1, MetaboAnalyst 5.0 and R 4.1.11. Results: (a) Body weight was significantly lower in the hypoxic obesity group than in the normoxic obesity group. (B) Differences in α-diversity and β-diversity were found in the faecal gut microbiota of mice of different body weight and altitude, the diversity of gut microbiota was higher in the normal group than in the obese group; the results of the comparison between the two groups showed that Faecalibaculum, Romboutsia, Lactobacillus and A2 were associated with obesity; Romboutsia was associated with hypoxia. (C) The metabolic profiles of faecal metabolites differed between groups, gut microbiota were associated with nucleotide and amino acid metabolism in the same body groups, gut microbiota were associated with lipid and amino acid metabolism in the same oxygen concentration groups. Conclution: (a) Gut microbiota diversity was reduced in obesity, Romboutsia is the dominant microbiota in the hypoxia group. (b) Gut microbiota were associated with nucleotide and amino acid metabolism in the same body weight groups, gut microbiota were associated with lipid and amino acid metabolism in the same altitude groups.