AUTHOR=Liu Yan , Wang Ziyan , Wang Ze , Zhou Jun , Han Jiaojiao , Lu Chenyang , Liu Bing , Yu Rongxian , Sun Xiaoling , Zhang Zhen , Wang Rixin , Su Xiurong 

TITLE=Rapid and simultaneous multiepitope antigen-based detection of Enterococcus by microscale thermophoresis and immunomagnetic separation

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 15 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1341451

DOI=10.3389/fmicb.2024.1341451

ISSN=1664-302X

ABSTRACT=Generally, enterococci bacteria cause nosocomial infections and are major indicators of bacterial contamination in marine bathing beach. However, a method for the rapid and simultaneous detection of multiple pathogenic enterococci has not been developed on account of the wide variety of pathogenic enterococci and their existence in complex matrices. In the present study, immunoinformatics tools were used to design a multi-epitope antigen for the detection of various pathogenic enterococci by using the sequence of dltD gene on enterococci lipoteichoic acid (LTA) surface, which is associated with toxicological effects. The multi-epitopes included enterococci such as Enterococcus faecalis, E.