AUTHOR=Yuan Chen , Zhao Xue , Feng Yawen , Chen Ligong , Lin Yidan , Li Tanqing , Song Qinye 

TITLE=Comparison of B cells' immune response induced by PEDV virulent and attenuated strains

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 15 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1344344

DOI=10.3389/fmicb.2024.1344344

ISSN=1664-302X

ABSTRACT=Porcine epidemic diarrhea virus (PEDV) is an acute, highly contagious enterovirus that infects pigs of all ages. The B cells are important for antigen presentation, antibody production and cytokine secretion to resist infection. However, the role of B cells in PEDV infection remains unclear. In this study, the effects of PEDV virulent (QY2016) and attenuated strains (CV777) on B cells sorted from neonatal piglets, nursery piglets and gilts were investigated. The results showed that the PEDV-QY2016 and PEDV-CV777 could significantly increase the expression of CD54 and CD27 in B cells from neonatal piglets. The percentages of CD80, MHC II, IgM expressed on neonatal piglet B cells infected with PEDV-QY2016 were significantly lower than those expressed on the B cells infected with PEDV-CV777. Both PEDV-QY2016 and PEDV-CV777 could stimulate IFN-α and GM-CSF secretion in neonatal piglet B cells, IL-1, IFN-α and IL-4 secretion in nursery piglet B cells, and IL-1, TGF-β secretion and GM-CSF in gilt B cells. Furthermore, both PEDV-QY2016 and PEDV-CV777 could induce the secretion of IgA, IgM and IgG in nursery piglet B cells, but could not induce the secretion of IgA, IgM and IgG in neonatal piglet B cells. And the secretion of IgA, IgM and IgG was significantly higher by the PEDV-CV777 strains infected B cells than that by the PEDV-QY2016 strains infected gilt B cells. In conclusion, the surface molecule expression, cytokine secretion and antibody production of B cells induced by PEDV are closely related to the pigs’ ages and the PEDV strain’ virulence.