AUTHOR=Yu Shuran , Xie Junjin , Guo Qiang , Yan Xia , Wang Yuxiang , Leng Tangjian , Li Lin , Zhou Jielong , Zhang Wenping , Su Xiaoyan 

TITLE=Clostridium butyricum isolated from giant panda can attenuate dextran sodium sulfate-induced colitis in mice

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1361945

DOI=10.3389/fmicb.2024.1361945

ISSN=1664-302X

ABSTRACT=<sec id="sec1"><title>Objective</title><p>Probiotics are beneficial to the intestinal barrier, but few studies have investigated probiotics from giant pandas. This study aims to explore the preventive effects of giant panda-derived <italic>Clostridium butyricum</italic> on dextran sodium sulfate (DSS)-induced colitis in mice.</p></sec><sec id="sec2"><title>Methods</title><p><italic>Clostridium butyricum</italic> was administered to mice 14 days before administering DSS treatment to induce enteritis.</p></sec><sec id="sec3"><title>Results</title><p><italic>Clostridium butyricum</italic> B14 could more effectively prevent colitis in mice than <italic>C. butyricum</italic> B13. <italic>C. butyricum</italic> B14 protected the mouse colon by decreasing the histology index and serum interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) levels, which improved intestinal inflammation-related symptoms. In addition, the treatment led to the regulation of the expression of <italic>Tifa, Igkv12-89,</italic> and <italic>Nr1d1</italic>, which in turn inhibited immune pathways. The expression of <italic>Muc4</italic>, <italic>Lama3, Cldn4, Cldn3</italic>, <italic>Ocln</italic>, <italic>Zo1, Zo2</italic>, and <italic>Snai</italic> is related the intestinal mucosal barrier. 16S sequencing shows that the <italic>C. butyricum</italic> B14 significantly increased the abundance of certain intestinal probiotics. Overall, <italic>C. butyricum</italic> B14 exerted a preventive effect on colitis in mice by inhibiting immune responses, enhancing the intestinal barrier and increasing the abundance of probiotic species. Thus, <italic>C. butyricum</italic> B14 administration helps regulate the balance of the intestinal microecology. It can suppress immune pathways and enhance barrier-protective proteins.</p></sec>