Efficacy of Lamium album as a natural fungicide: impact on seed germination, ergosterol, and mycotoxins in Fusarium culmorum-infected wheat seedlings

Fusarium culmorum is a major wheat pathogen, and its secondary metabolites (mycotoxins) cause damage to plants, animals, and human health. In the era of sustainable agriculture, eco-friendly methods of prevention and control are constantly needed. The use of plant extracts as biocontrol agents has gained popularity as they are a source of active substances that play a crucial role in fighting against phytopathogens. This study evaluated the impact of Lamium album on wheat seed germination and seedling growth. In a pot experiment, the effect of L. album on wheat seedlings artificially inoculated with F. culmorum was evaluated by measuring seedling growth parameters, and by using chromatographic methods, ergosterol and mycotoxins levels were analyzed. The results showed that the phytotoxic effect of L. album flower extracts on wheat seed germination and seedling growth was concentration dependent. The radicle length was also reduced compared to the control; however, L. album did not significantly affect the dry weight of the radicle. A slight phytotoxic effect on seed germination was observed, but antifungal effects on artificially infected wheat seedlings were also confirmed with the reduction of ergosterol level and mycotoxins accumulation in the roots and leaves after 21 days of inoculation. F. culmorum DNA was identified in the control samples only. Overall, this study is a successful in planta study showing L. album flower extract protection of wheat against the pathogen responsible for Fusarium crown and root rot. Further research is essential to study the effects of L. album extracts on key regulatory genes for mycotoxin biosynthetic pathways.

Efforts to combat Fusarium infections in wheat have involved substantial investments in genetic resistance, crop rotation, and other practices (Özdemir, 2022).However, complete host resistance remains elusive (Wagacha and Muthomi, 2007), underscoring the ongoing importance of fungicides (Zubrod et al., 2019).Synthetic fungicides, while effective, pose risks to human health and the environment (Castro et al., 2020;Seepe et al., 2021), forcing the exploration of natural alternatives, which are vital for sustainable crop cultivation.Plant extracts are recognized as safe and offer a promising solution (Abdallah et al., 2018), containing a blend of compounds, including phenolic acids, flavonoids, tannins, terpenes, and alkaloids, that can collaborate to inhibit the growth of phytopathogenic fungi (Deresa and Diriba, 2023).Moreover, plant extracts are highly effective against a wide range of pests and diseases, are relatively easy and cheap to produce, and show low toxicity against non-target organisms, including humans (Suteu et al., 2020).Significantly, these compounds not only limit fungal growth by inhibiting ergosterol biosynthesis as an indicator of fungal biomass (Perkowski et al., 2008) but also inhibit mycotoxin biosynthesis and trigger plant defense responses (Acheuk et al., 2022).However, to monitor the in vivo antifungal properties of a plant extract, it is essential to evaluate the phytotoxic effects on the target plant that requires protection, considering that certain studies have indicated their potential adverse effects.
L. album is a medicinal plant distributed in Europe, Asia, and Africa (Chipeva et al., 2013).To understand the mechanisms of action, researchers identified its chemical constituents and found that it has various chemicals, including iridoid glycosides, phenolic acids, flavonoids, alkaloids, triterpenes, and other compounds with diverse biological properties (Damtoft, 1992;Pereira et al., 2012;Pourmirzaee et al., 2019;Sulborska et al., 2020;Uwineza et al., 2021).In recent in vitro research, L. album flower extracts demonstrated promising effects against Fusarium species, emphasizing its versatile applications (Uwineza et al., 2023).Most studies focus on plant extracts' fungicidal or fungistatic effects (García-Ramírez et al., 2023).However, they often overlook the impact of these extracts on mycotoxin production by pathogenic fungi and phytotoxic effects on the crops.Furthermore, most of these studies are conducted in vitro (Kursa et al., 2022;Uwineza et al., 2022), while in vivo experiments are necessary to confirm the biocontrol effects of such plant extracts, as the data available is limited.In addition, for the plant extract to be effective, accurate identification of the pathogen is crucial.Identifying pathogens based on observed symptoms can be challenging, especially at the early stages of disease.Therefore, molecular methods, particularly those based on polymerase chain reaction (PCR), offer superior detection capabilities.The technique is a rapid and sensitive tool for detecting and identifying targeted DNA molecules of pathogens in plant tissues (Pszczółkowska et al., 2013).
The study aimed to assess the efficacy of L. album flower extract [obtained by using supercritical fluid carbon dioxide (SC-CO 2 )] on wheat seed germination and analyze its antifungal action against F. culmorum in artificially infected wheat seedlings.Our previous research has shown a promising in vitro effect of L. album against F. culmorum and F. proliferatum (Uwineza et al., 2023), and the literature has indicated that infections in seedlings and basal stems often originate from seed or soil-borne inoculum, leading to significant yield losses due to damaged seedlings, pre-harvest lodging, and impaired grain filling (Gebremariam et al., 2020).Therefore, controlling Fusarium infection from the early stage may promise better harvesting.

Results
. E ect of Lamium album on wheat seed germination and seedling growth The effect of L. album flower extract on wheat seeds was evaluated after 7 days of incubation.The results showed that L. album extracts inhibited wheat seed germination in a concentration-dependent manner (Table 1).The germination percentage was calculated using the Equation 1, where the control sample had a 100% germination percentage, while the treated samples showed a significant difference with a 65% germination percentage for 5% of extracts, while for 10% of extracts, the germination percentage was 45%.Additionally with the Equation 2, L. album flower extract reduced the seedling length vigor index from 669.50 to 303.86 compared to the control (2,860.00).It also decreased the seedling weight vigor index from 2.83 to 1.84 compared to the control value of 3.92 obtained using the Equation 3.
Furthermore, the concentration of L. album significantly influenced both shoot length (F = 259.3,p < 0.0001) and root length (F = 282.5,p < 0.0001) of wheat seedlings, as depicted in Figures 1, 2. However, there was no significant difference in dry matter (F = 0.7018, p = 0.5209).With an increase in L. album concentration, there was a noticeable decrease in shoot and root length.
In specific instances, such as at a 10% L. album concentration, the germinated seeds resulted in shoot and root lengths of 4.55 and 2.20 cm, respectively.In contrast, at a 5% L. album concentration, the shoot and root lengths were 6.975 and 3.32 cm, respectively, both shorter than the untreated control with shoot and root lengths of 12.88 cm and 15.73 cm, respectively.Values with different letters are statistically different (values obtained from the mean results of the treatment, α = 0.05).

FIGURE
The e ect of di erent concentrations of L. album extract ( and %) compared to the untreated control: (A) the shoot length (cm), (B) root length (cm), and (C) dry matter (mg) of seedlings after days of growth.Values with di erent letters are statistically di erent (mean ± standard deviation, α = .).

FIGURE
The e ects of di erent concentrations of L. album flower extract ( % and %) compared to the untreated control (treated with water) on seedlings' shoot and root length after days of incubation.  .Comparative e ects of L. album extract on the physiological growth parameters of wheat seedlings in the presence and absence of Fusarium culmorum infection Physiological growth parameters of wheat seedlings were variably influenced by L. album flower extract and F. culmorum infection.With the data collected after 21 days of artificial infection of wheat seedlings, there are slight differences between the seedling root weight of the pathogen-inoculated control (Control_FC) and the inoculated and treated with 5% and 10% of L. album extract (5% _FC and 10% _FC) with a mass drop of approximately (−0.3 g).However, root weight loss was observed in non-infected seedlings treated with L. album extract (5% and 10% NC), with a mass drop of (−1.8 g) compared to the control.Similar effects were also observed in root length, except for non-infected seedlings treated with 10% of extracts (10% NC), which showed the longest roots (Table 2, Figure 3).The samples treated with 10% extract showed a stunting effect of F. culmorum on seedling root development, where the infected seedlings (10% _FC) showed a decrease in root length compared to non-infected seedlings (10% NC) but increased root weight.This reduction may signify a positive impact of L. album flower extract in combating Fusarium.Shorter roots could be a defense response, limiting Fusarium invasion and strengthening plant resistance.This observation underscores the potential antifungal efficacy of L. album extract.
In summary, L. album flower extract and F. culmorum infection affected the whole plant.Five percent extract yielded 15.32 g of fresh weight of the seedling compared to 11.25 g for 10% extract.Furthermore, total plant height was also affected, and 5% of samples showed greater height than the 10% samples.In addition, visual differences between the seedlings were observed, with the infected seedlings displaying infection symptoms and non-infected wheat seedlings showing no symptoms (Figure 4).

. Fusarium spp. identification
Molecular identification of all fungal strains that might be present in the collected seedlings was performed using sequence analysis of the PCR-amplified fragments PCR.The DNA regions were amplified with EF728M and Tef1α primers and were subsequently sequenced.Amplified DNA fragments were compared with reference genes from the GenBank Database to confirm species identification.The results showed that the isolated strains in the contaminated wheat seedlings were Fusarium spp., as expected.However, isolates in the roots of the control group were F. culmorum (Strains -FC1Croot, and FC-C2root with 96.4% and 96.81% identical bases, respectively).For roots of the seedlings treated with L. album extracts, the identified fungi were F. oxysporum (Strains -FC5.4.1R and FC10.5.1G with 97.86% and 98.2% identical bases, respectively).In addition, no significant similarities in fungal species were found in infected seedling leaves as well as in non-infected seedling leaves and roots (data not shown).  .

E ect of L. album flower extracts on Fusarium growth
The influence of extracts on the growth and development of Fusarium was measured by ergosterol (ERG) content, which is an indicator of fungal biomass.The HPLC/PDA analysis successfully detected the presence of ergosterol in both the roots and leaves of the harvested wheat seedlings (Table 3).ERG was present both in non-infected and infected seedlings.In the non-infected seedlings, the control without the extract showed the highest ERG level in leaves and roots, the ones with 5% of the extract being lower and the lowest for 10%, meaning that endophytic fungi were present in the plant tissue.In both cases (roots and leaves), the extract inhibited fungal development inside the plant.ERG levels in those seedlings were significantly lower compared to the infected seedlings.Similarly, in the case of artificially infected seedlings, the control without the extract had the highest level of ergosterol in roots and leaves.However by using the Equation 4, a significant ERG reduction was observed in the samples treated with 10% extract, with the highest reduction of up to 84.57% in the leaves and 65.52% in the roots.For the 5% extract, ergosterol was diminished by 52% in the roots and 79.31% in the leaves.This showed that the application of L. album extracts led to a notable lowering of ERG levels in the infected seedlings.Statistically, significant differences were observed between the 5% and 10% of extract, as determined by ANOVA with post-hoc Duncan test at p < 0.05.
. E ects of L. album flower extracts on mycotoxins biosynthesis in wheat seedlings artificially infected with F. culmorum Mycotoxin biosynthesis is one of the challenging phenomena associated with fungal infection.Our study analyzed the effects of L. album flower extracts on the mycotoxins content in the roots and leaves of seedlings artificially infected with F. culmorum as well as in non-infected seedlings using the LC-MS/MS technique.Four commonly synthesized mycotoxins of F. culmorum, namely DON, 3-and 15-AcDON, ZEN, and ZEN-14S were quantified (Table 4).Notably, no mycotoxins were identified in the noninfected wheat seedlings.
A reduction of mycotoxin accumulation was noted in the seedlings treated with L. album flower extracts.In the roots, there were significantly higher amounts of all mycotoxins compared to the leaves, with lower production of DON and 3-and 15-AcDON than ZEN and ZEN-14S.L. album caused complete inhibition (100%) of DON and 3-and 15-AcDON in the roots.While ZEN was entirely inhibited in the leaves, in the roots decreased within the range of 10.00 to 7.28 ng/g compared to the control value of 56.01 ng/g, representing a reduction of 82.14% for the 5% and 87.41% for the 10% of L. album extract.Regarding ZEN-14S, complete inhibition occurred in the leaves, but in the its concentration decreased within the range of 12.90 to 10.87 ng/g compared to the control value of 51.68 ng/g.This reduction was equivalent to 75.03% for 5% concentration and 78.97% for 10%.Overall, a significant impact of L. album on mycotoxin biosynthesis was shown, resulting in a noteworthy reduction in mycotoxin concentrations compared to the control, regardless of the extract concentration (differences were insignificant at p < 0.05 for all produced mycotoxins).

. Correlation between ergosterol and the produced mycotoxins (Pearson r correlation)
Pearson correlation (Pearson's r) assessed the linear relationship between ergosterol content and mycotoxins in the infected wheat seedlings.The analysis showed that ergosterol content positively correlated with the synthesized mycotoxins, and it was statistically significant at p < 0.05.Furthermore, highly positive correlations were found between the concentrations of DON and 3-and 15-AcDON and between ZEN and ZEN-14S (Figure 5).Compared to this, weaker positive correlations were observed between DON and ZEN-14S (r = 0.57).

Discussion
The germination capability of wheat seeds serves as a crucial indicator reflecting their quality, viability, and overall potential (Hassani et al., 2019).In our study, L. album decreased seed germination and seedling growth after seven days of incubation compared to the control.This effect was in agreement with other studies, showing reduced wheat germination of seeds treated with Ulva linza or Corallina officinalis seaweeds (Hamouda et al., 2022), Flaveria bidentis (Dai et al., 2022) and aqueous extracts of weed plants: Hyptis sauveolens (L.), Ricinus communis (L.), Alternanthera sessilis (L.), Ipomoea carnea (Jacq), Malachra capitata (L.), and Cymbopogon citrutus (Stapf) (Joshi and Joshi, 2016).The decrease in germination percentage could be attributed to the potential phytotoxic or allelopathic effects of the extracts (Joshi and Joshi, 2016;Ma et al., 2011;Dai et al., 2022;Hamouda et al., 2022;Rys et al., 2022), such as the harmful or inhibitory impact that plant extracts may have on plant tissues or biological processes (Ma et al., 2011;Werrie et al., 2020) or chemical interactions between plants, which may be direct or indirect, beneficial or detrimental (Joshi and Joshi, 2016;Aurelio et al., 2022).The control group exhibited the longest wheat seedlings, with the lowest recorded dry weight compared to the treated wheat seeds.Although plant extracts mildly inhibited seedling weight loss (Figure 1), the effect was not significantly different from the control at p FIGURE Pearson correlation between ergosterol content and biosynthesized mycotoxins in the infected wheat seedlings.
Frontiers in Microbiology frontiersin.org< 0.05.The observed reduction in plant growth under natural conditions may be attributed to seed competition for water, minerals, oxygen, or root system space (Rys et al., 2022).Some plant extracts, despite their beneficial properties, can also contain compounds that hinder seed germination or seedling growth (Bota et al., 2021).Hamouda et al. (2022) investigated the effect of wheat seed priming with the aqueous extracts of Ulva linza and Corallina officinalis seaweeds; higher concentrations inhibited seedling growth and caused specific chromosomal abnormalities, while low concentrations of seaweed extract as a priming treatment improved wheat seedling growth, physiological features and had a greater capacity to boost bioavailable macro-and micronutrients to the plant (Hamouda et al., 2022).F. culmorum can impact plants across different developmental stages, leading to seedling blight and root rot, hindering seedling emergence and overall plant development (Pastuszak et al., 2021).This pathogenic fungus infects wheat plants, leading to foot rot symptoms, particularly affecting the roots and lower stems.The severity of the disease varies, with early infections causing preand post-emergence seedling death, while later infections result in brown lesions on the lower stems and tiller abortion (Scherm et al., 2013).While FHB is often a primary focus in research due to its economic impact and mycotoxin production (Bottalico and Perrone, 2002;Scherm et al., 2013;Antalová et al., 2020), foot rot caused by F. culmorum is a significant issue that requires attention (Scherm et al., 2013).The presence of L. album and F. culmorum infection caused stress to the growing seedlings and by responding to these biotic stresses, seedlings produced many short roots that have influenced the weight and length of the roots (Table 2).Roots are crucial for nutrient uptake and overall plant health.The observed decrease in root length for the seedling treated with 10%_FC may be a defense response triggered by the plant in the presence of Fusarium.L. album extract might influence this response positively, aiding the wheat seedling in resisting fungi infection.Moreover, shorter roots may limit the pathogen's ability to invade the plant's vascular system, acting as a physical barrier.While a decrease in root length might be perceived as an adverse effect of Fusarium infection in wheat, as noted in a study conducted by Saad et al. (2023), it could be a strategic adaptation to strengthen the plants' defense mechanisms induced by plant extract against Fusarium spp, ultimately contributing to enhanced resistance.Previous research showed that seedlings exhibiting moderate vigor were more susceptible to infection than those with high vigor and exhibited a significant height increase when subjected to inoculation with F. graminearum spores (Zhou et al., 2018).Similarly, the application of essential oil disturbed the growth of seedlings' roots and reduced the vigor index (Grzanka et al., 2021).Seedlings protected with L. album extracts showed no symptoms.In contrast, the control samples exhibited visible symptoms, such as root browning, yellowing of the first leaves, and visible mycelia on the above-ground part of the seedlings (Figure 4).Previous studies reported that the early infection reduced wheat seedling growth as F. culmorum can effectively penetrate seedling roots, migrate from hypocotyl to the upper stem internodes and leaves, colonize the host's tissue and cells, block the vascular bundles, disturb nutritional supply and metabolic processes (Kthiri et al., 2021).In another study, three durum wheat genotypes at seedling and full anthesis stage F. culmorum infection significantly decreased chlorophyll a, b, and carotenoid contents in the leaves (Pastuszak et al., 2021).L. album may also cause adverse effects against F. culmorum, inducing toxin accumulation, especially under conditions unfavorable for fungal growth or at low extract concentrations (Tretiakova et al., 2022).
Ergosterol, being integral to fungal cell membranes, serves as an indicator of fungal biomass and is vital for maintaining cell function and integrity (Perkowski et al., 2008;Uwineza et al., 2022).Severe ERG depletion may influence cell growth and proliferation (Abhishek et al., 2015).Here, the effect of L. album extract was evident, as shown by the ERG reduction in infected seedlings.Low ERG levels in non-infected seedlings could be attributed to endophytic fungi-microorganisms living within plant tissues without visible disease symptoms (Abaya et al., 2021;Noel et al., 2022).PCR-based identification confirmed F. oxysporum in the root of infected samples treated with L. album flower extracts.Also, no mycotoxins were identified in those samples.Noel et al. (2022) reported F. oxysporum as one of the fungal endophytes that significantly increased seed weight and lowered the accumulation of DON compared to F. graminearum-infected wheat heads without endophyte (Noel et al., 2022).L. album extract suppressed the growth of pathogenic and endophytic fungi in a concentration-dependent manner.This inhibitory effect could be attributed to the active compounds in L. album flower extracts (Pereira et al., 2012;Pourmirzaee et al., 2019;Sulborska et al., 2020;Uwineza et al., 2021).Detrimental impact on ERG content confirmed a significant disruption or biosynthesis blockage within the plasma membrane of F. culmorum.This finding is in agreement with some of the previous studies that have reported a decrease in ERG content in infected samples after the application of plant extracts (Abhishek et al., 2015;Uwineza et al., 2022).Leaf extract of Solanum torvum Swartz.decreased ERG production by the Fusarium verticillioides strain (Abhishek et al., 2015).Previous in vitro study also showed L. album flower extract activity against F. culmorum and F. proliferatum, reducing ERG content (Uwineza et al., 2023).
Mycotoxigenic fungi can simultaneously produce various compounds (Streit et al., 2012).To monitor whether L. album extract alters the mycotoxin biosynthesis in wheat seedlings, an analysis was conducted, revealing that the content of mycotoxins, including DON, 3-and 15-AcDON, ZEN, and ZEN-14S, commonly associated with F. culmorum contamination (Bryła et al., 2018;Uwineza et al., 2022), were reduced both in roots and leaves of seedlings treated with L. album extract.Abbas and Yli-mattila (2022) showed that the methanolic extract of the medicinal plant Zanthoxylum bungeanum successfully decreased F. graminearum growth and abrogated DON production in wheat heads (Abbas and Yli-mattila, 2022).Similarly, Shcherbakova et al. (2018) found no increase in DON, AcDON, and ZEN content when F. culmorum and F. graminearum developed on extract-treated wheat seeds and seedlings (Shcherbakova et al., 2018).
DON and its acetylated forms 3-and 15-AcDON were completely inhibited by L. album.Correlations between DON and its acetylated forms were highly significant, which is in accordance with the previous research (Sunic et al., 2021), where DON highly Frontiers in Microbiology frontiersin.orgcorrelated with 3-AcDON in winter wheat, and its production by F. culmorum is believed to play a role in pathogenesis (Morimura et al., 2020).Mycotoxin accumulation affects germination rates, seedling growth, pathogen aggressiveness, and overall disease severity (Bruins et al., 1993;Scherm et al., 2011;Winter et al., 2019).Scherm et al. (2011) showed that DON is an aggressiveness factor in F. culmorum stem base infections of durum wheat, whereby disease incidence was decreased by 40%−80% when the trichothecene regulatory gene Tri6 was silenced (Scherm et al., 2011).Similarly, Winter et al. (2019) showed that high DON-and 3-AcDONproducing isolate led to more severe symptoms and 20 times more significant colonization of the stem base than isolates that produced less DON/3-AcDON (Winter et al., 2019).A 10-fold higher accumulation of ZEN and ZEN-14S in infected seedling roots was observed compared to DON, which is consistent with previous findings (Ksieniewicz-Wozniak et al., 2021).It can be explained by L. album extract ability to regulate the enzymes involved in the synthesis or modification of ZEN and its derivatives compared to the ones that process DON.Additionally, the accumulation of ZEN and ZEN-14S over DON may be favored, according to a study that investigated the effect of compactin on mycotoxin production and the expression of associated biosynthetic and regulatory genes in toxigenic F. culmorum.Their findings showed that compactin had a suppressing effect on DON and ZEN differently due to its effect on various genes involved in the biosynthesis of these mycotoxins (Stakheev et al., 2022).These intricate interactions could lead to differential expression of genes related to mycotoxin biosynthesis and modification, resulting in the observed variations in mycotoxin levels (Scherm et al., 2011).However, even though ZEN and ZEN-14S were highly synthesized, the application of L. album significantly reduced them compared to the control.ZEN was reduced to 82.14% for 5% and 87.41% for 10% of L. album extract, and ZEN-14S was decreased to 75.03% for 5% and 78.97% for 10%, confirming the mechanism of action of L. album extract on the reduction of mycotoxin biosynthesis (Jafarzadeh et al., 2022).A strong correlation between ZEN and ZEN-14S was observed, while a weaker correlation occurred among DON, ZEN, and ZEN-14S, which is in accordance with previous research (Birr et al., 2021).ZEN can be modified (e.g., via conjugation) in plants, fungi, and animals through phase I and phase II metabolism (Veršilovskis et al., 2019).This conjugated form (ZEN-14S) is approximately 60 times more estrogenic than ZEN and can be easily hydrolyzed to ZEN in the gastrointestinal tract, thereby increasing exposure to ZEN (Veršilovskis et al., 2019).A high ZEN-14S/ZEN ratio in the malted wheat suggests a possibility of Fusarium converting ZEN into a phase II metabolite through sulfation reactions (Ksieniewicz-Wozniak et al., 2021).Wheat infections occur during or shortly after flowering (Wegulo, 2012); however, our study revealed that mycotoxin accumulation can start at the initial stages of infection.
In this study, we have presented the dual effect of L. album extracts, which acted as antifungal on F. culmorum in wheat seedlings and showed the phytotoxic effect on seed germination.Composition and concentration of L. album extracts can be essential here, as they have not been analyzed until now, but similar results have been reported previously (Bayar and Yilar, 2019;El-Alam et al., 2020;Mehdizadeh et al., 2020).El-Alam et al. (2020) reported that all tested essential oils (EO) presented antifungal properties against F. culmorum by direct contact and volatility assays.However, all tested EO have also shown a phytotoxic activity, either by inhibiting seed germination or affecting root elongation of rye and lettuce (El-Alam et al., 2020).Therefore, carefully considering and optimizing the extract concentration and application method is advised to ensure that the antifungal benefits outweigh any potential phytotoxic effects of the plant extracts.

Conclusions
The present study highlights the dual impact of L. album flower extracts on wheat seed germination, seedling growth, and Fusarium infection control.While exhibiting phytotoxic and allelopathic effects that hindered seed germination and seedling growth, the extracts effectively controlled F. culmorum infection and reduced visible symptoms.The anti-mycotoxigenic potential of L. album flower extracts was evident in the complete inhibition of DON, 3-and 15-AcDON, and the substantial reduction of ZEN and ZEN-14S in artificially infected wheat seedlings.The reduction of ergosterol content in the seedling roots confirmed the disruptive impact on F. culmorum, emphasizing the potential of L. album as a biocontrol agent against Fusarium mycotoxins.The study highlights intricate molecular mechanisms at play and emphasizes the need for further exploration of the effect of L. album extracts on key regulatory genes for mycotoxin biosynthesis.Despite the observed phytotoxicity, the study suggests the potential of L. album extracts as a biocontrol agent, stressing the importance of optimizing concentration and application methods in agricultural practices.Overall, this research contributes valuable insights into the sustainable use of L. album in combating Fusarium species in wheat cultivation, with further exploration required for effective implementation.

Materials and methods . Plant extraction
The material used for the extraction was dried Lamium album flowers purchased from a certified Polish company called Dary Natury located in Podlaskie Voivodeship of Poland (53 • 4′10.98 latitude and 22 • 58′2.87 longitude).The extraction method used was the supercritical fluid extraction technique using methanol as a cosolvent, as described in the previous study (Uwineza et al., 2022).Subsequently, the obtained extracts were evaporated in a vacuum evaporator (Buchi R-215 Rotary Evaporator System, Germany) at 40 • C to eliminate the methanol; the dried extract was reconstituted in a measured volume of distilled water and stored at −18 • C until antifungal assays.

. Studied material
Healthy wheat grains (Triticum aestivum L. cultivar Arkadia) were provided by the Plant-Pathogen Interaction Team, Institute of Plant Genetics, Polish Academy of Sciences, Poznan, Poland.
Fungal isolate of F. culmorum KF 846 was obtained from the collection of the Plant-Pathogen Interaction Team, Institute of Plant Genetics, Polish Academy of Sciences, Poznan, Poland.The tested strain was cultured in 9 cm Petri dishes on potato dextrose agar medium (PDA, BioShop, Burlington, ON, Canada) at 28 • C for 7 days.

. Standards, chemicals and reagents
Carbon dioxide (CO 2 , SFE grade), contained in a dip tube cylinder, was purchased from Air Products Sp, Poland.Methanol for HPLC-super gradient was purchased from POCh (Gliwice, Poland).Acetonitrile, methanol, and water for LC-MS grade were acquired from POCh (Gliwice, Poland).Dream Taq green PCR master mix kit was purchased from Thermo Scientific (Vilnius, Lithuania).Analytical standards including ERG, ZEN, DON, 15-AcDON, and 3-AcDON were purchased in ready-to-use solutions from Romer Labs (Tulln, Austria), and ZEN-14S (100 µg/mL) purchased in Aokin (Berlin, Germany).Depending on solubility, the standards were dissolved in acetonitrile.All standards were stored in amber glass vials at approximately −20 • C. A mixture of all standards necessary for a particular analytical run was prepared immediately before the analysis.
. E ect of Lamium album flower extract on seed germination and seedling growth The effect of Lamium album flower extract on the germination of wheat kernels was determined according to Al-Khafajy et al. (2022) with some modifications.Ten mL solution of L. album flower extract was prepared at 5% and 10% concentrations, then added to sterilized glass plates with tissue paper containing sterile 10 grains/plate; the control samples were prepared with 10 mL of distilled water, and aseptically prepared plates (four plates for each treatment) were incubated under controlled whirlpool chamber at 28 • C for 7 days.Germination percentage was calculated using the following formula (Equation 1): Germination percentage (%) = (number of healthy seedlings/total number of the seed)×100 (1) The root and shoot length of the seedlings were measured after seven days of germination.Then, all seedlings were collected and freeze-dried to determine the seedling dry weight (g).Also, seedling length vigor index (SLVI) and seedling weight vigor index (SWVI) relations were determined by the following Equations 2, 3 (Hassani et al., 2019): SLVI = (mean shoot length (cm) + mean root length (cm)) ×percentage of seed germination) (2) SWVI = dry weight seedling (g) × percentage of seed germination (3) . E ect of Lamium album flower extracts on wheat seedlings against F. culmorum in controlled conditions Ten wheat grains were surface sterilized using a 0.6% (v/v) bleach (sodium hypochlorite) solution, followed by rinsing with distilled water three to four times.Subsequently, 10 mL of Lamium album flower extract was prepared at 5% and 10% concentrations, then added to sterilized glass plates with tissue paper containing 10 sterile grains/plate.The control samples were prepared with 10 mL of distilled water.Aseptically prepared plates (four plates for each treatment) were incubated under a controlled whirlpool chamber at 28 • C for 7 days to germinate.Plastic pots (13 cm in height) were prepared and filled with a sterile potting mix of soil, sand, and horticulture vermiculite fine (60%, 30%, and 10%; v/v/v) each.Each pot was then planted with a germinated grain (7-day-old seedling) treated with water, and grains treated with L. album extracts at 5 and 10% were marked accordingly.The pots were divided into two groups (four pots for each treatment): the first group served as the control, while in the second group, each pot was inoculated with 100 ml of the prepared spore suspension of F. culmorum containing 10 6 conidial spores/mL after 10 days of potting.All the pots were arranged on the control room benches, maintained under a natural photoperiod (16 h of light/8 h of darkness) at 23 ± 4 • C and with 45% humidity.The seedlings were irrigated with sterile water (100 ml) after 2 days.Daily observations were made to detect any disease symptoms, and the experiment was concluded on the 21 st day after inoculation.Both underground (roots) and aerial parts (leaves) of the seedlings were measured (length and weight), collected separately, and stored for further analysis.The experiment comprised four replicates for each treatment, including infected and non-infected wheat seedlings treated with L. album flower extracts at 0% control, as well as 5% and 10% concentrations.This process was repeated for reproducibility, and the results represent the mean of two distinct experiments.

. Isolation and purification of fusarium species
To isolate Fusarium spp.from the collected seedling parts (roots and leaves) from each treatment, a 0.6% (v/v) bleach (sodium hypochlorite) solution was used for surface sterilization, followed by rinsing with distilled water three to four times.Subsequently, the sterilized samples were placed on sterile plates with PDA and incubated at 28 • C in the dark for 7 days.The resulting colonies were transferred until purified isolates were obtained.All fungi were maintained on PDA medium at 4 • C and sub-cultured monthly until DNA analysis.
. . .DNA extraction and Fusarium sp.identification Genomic DNA was extracted using a modified method with the hexadecyltrimethylammonium bromide (CTAB) (Urbaniak et al., 2020).Mycelium scraped from a 7-day-old PDA culture was ground to a fine powder with liquid nitrogen, and 800 µl of CTAB solution was added to each Eppendorf tube, 150 µl of chloroform-isoamyl alcohol (24:1), then mixed gently.Then, 4 µl of β-mercaptoethanol was added, and samples were incubated in the water bath for 20 min at 65 • C.After cooling for 5 min, 150 µl of chloroform-isoamyl alcohol (24:1) was added and mixed gently.The samples were centrifuged for 20 min at 12,000 rpm and 4 • C. The supernatant was supplied with 60 µl of 3M sodium acetate and 1,000 µl of ethyl alcohol and precipitated 20 min at −23 • C.Then, the samples were centrifuged for 20 min at 12,000 rpm, air dried for 1 h, and re-dissolved in 150 µl of TE buffer pH 8.
For sequence analysis, PCR-amplified DNA fragments were purified with exonuclease I (Vilnius, Lithuania) and Fast AP thermosensitive alkaline phosphatase (Vilnius, Lithuania) using the following program: 15 min at 37 • C and 15 min at 85 • C. The strand was labeled using a BigDye Tm Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific, Vilnius, Lithuania), according to the previously described protocol (Stepień and Waśkiewicz, 2013) and precipitated with 70% ethanol.Sequence reading was performed using Applied Biosystems equipment.Sequences were edited using Chromas v. 1.43 (Technelysium, Tewantin, Australia) and analyzed using the BLASTn algorithm.Sequences were deposited in GenBank and will be publicly available.
. .Chemical analysis . . .The ERG content analysis After collecting the seedling's roots and leaves, the materials were lyophilized and ground into fine powder.The concentration of ERG in the samples was determined by comparing the retention time of the analyte with that of an external standard (Waśkiewicz et al., 2014).The method had a detection limit of 10 ng/g.The ergosterol reduction percentage was calculated using the following formula (Equation 4): ERG reduction (%) = [(control-treatment)/control]×100 (4)

. . . Mycotoxins analysis
Mycotoxin extraction was performed by adding 5 mL of the extraction solvents (acetonitrile: water, 86:16, v/v) to 0.5 g of dried roots or leaves of the infected and non-infected wheat seedlings, vortexing (for about 30 s) and mixing using a horizontal shaker for 24 h.After extraction, the samples were centrifuged at 7,500 rpm for 10 min.Then, approximately 2 mL of supernatant was filtered through a 0.2 µm syringe filter (Chromafil, Macherey-Nagel, Duren, Germany) and poured into vials for chromatographic analysis.For the analysis, the method reported by Uwineza et al. ( 2022) was followed with some modifications (Perczak et al., 2020).The compounds were quantitatively analyzed using multiple reaction monitoring.The mycotoxin concentrations (µg/g) were calculated using a calibration curve based on commercial singlecomponent preparations of DON,ZEN, All samples were analyzed in triplicate.

. . Statistical analysis
The experimental design consisted of four replicates of each treatment, and each experiment was repeated twice.The results were interpreted as mean standard deviation.Analysis of variance (ANOVA-One way) was applied for the statistical analysis of experimental data using the Statgraphics v. 4.1 software package (Graphics Software System, STCC, Inc., Rockville, MD, USA), and each experimental value was compared with the corresponding control.Where there was statistical significance (p < 0.05), the mean values were further separated using Duncan's multiple-range test.GraphPad Prism9 was used for the graphs.

FIGURE
FIGUREDi erences in root phenotype among the harvested wheat seedlings after days of infection: (A) comparative observation of the infected and non-infected seedlings, (B) non-infected seedlings, and (C) infected seedlings.

FIGUREF
FIGUREF.culmorum symptoms in the treated seedlings and the control group after days of infection.

TABLE Comparative e
ects of L. album on wheat seed germination.

_FC and 10% _FC -
TABLE E ect of L. album extracts on the growth of infected and non-infected wheat seedlings.seedlings treated with extracts and inoculated with F. culmorum, Control_NC -seedlings treated with water without the inoculation of F. culmorum,

and 5% NC and 10% NC -
seedlings treated with extract without the inoculation of F. culmorum.

TABLE Comparative
e ects of L. album on the ergosterol content [µg/g] and its reduction [%] between the infected and non-infected wheat seedlings.Ergosterol content [µg/g]All the values are the mean of four replicate ± standard deviation.Values with different letters are statistically different (α = 0.05); * Leaves_NI: leaves of non-infected seedlings; Roots_NI: roots of non-infected seedling; Leaves_I: leaves of the infected seedlings; Roots_I: roots of the infected seedlings.