AUTHOR=Zhao Shuai , Li Hanyan , Sumpradit Tawatchai , Khan Aman 

TITLE=Enhancing biomass conservation and enzymatic hydrolysis of sweet sorghum bagasse by combining pretreatment with ensiling and NaOH

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 15 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1370686

DOI=10.3389/fmicb.2024.1370686

ISSN=1664-302X

ABSTRACT=Lignocellulosic pretreatment is an important stage in biomass utilization, which usually caused high input. In this study, a low-cost method by combined ensiling and NaOH was developed for Lignocellulosic pretreatment. Sweet sorghum bagasse (SSB) was ensiled for 21 days and then treated with diluted NaOH (0, 1, and 2%) for fermentation. The results showed that application of Lactobacillus plantarum (L) reduced fermentation losses of the silages, mainly including low water soluble carbohydrate (WSC) and ammonia nitrogen loss. Meanwhile, the application of Lactobacillus plantarum and ensiling enzyme (LE) promoted lignocellulosic degradation, as evidenced by low neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin (ADL) and hemicellulosic (HC) contents. The dominant bacterial genera were Lactobacillus, uncultured_bacterium_f_Enterobacteriaceae and Pantoea after silage, which corresponded to the higher lactic acid, acetic content and lower pH.The reducing sugar yields of SSB increased after combined pretreatment of silage and NaOH, and further enhanced by the 2% NaOH application, as evidenced by high reducing sugar yield and microstructure damage, especially in L-2% NaOH group and LE-2% NaOH group, in which the reducing sugar yields were 87.99% and 94.45%, respectively, compared with that of no additive control (CK)-0 NaOH group.Therefore, this study provides an effective method for sweet sorghum bagasse pretreatment to enhance biomass conservation.