AUTHOR=Lin Jie , Zhang Ruyi , Liu Huaie , Zhu Yunzhen , Dong Ningling , Qu Qiu , Bi Hongyan , Zhang Lihua , Luo Ou , Sun Lei , Ma Mengjuan , You Jing 

TITLE=Multi-omics analysis of the biological mechanism of the pathogenesis of non-alcoholic fatty liver disease

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 15 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1379064

DOI=10.3389/fmicb.2024.1379064

ISSN=1664-302X

ABSTRACT=Background: Non-alcoholic fatty liver disease (NAFLD) is a type of liver metabolic syndrome.Employing multi-omics analyses encompassing the microbiome, metabolome and transcriptome is crucial for comprehensively eclucidating the biological processes underlying NAFLD.Methods: Hepatic tissue, blood and faecal samples were obtained from 9 NAFLD model mice and 8 normal control mice. Total faecal microbiota DNA was extracted, and 16S rRNA was amplified, to analyse alterations in the gut microbiota (GM) induced by NAFLD. Subsequently, diagnostic strains for NAFLD were screened , and their functional aspects were examined. Differential metabolites and differentially expressed genes were also screened, followed by enrichment analysis . Correlations between the differential microbiota and metabolites, as well as between the DEGs and differential  metabolites were studied. A collinear network involving key genes-,microbiota-and metabolites was constructed.