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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Systems Microbiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1427143
This article is part of the Research Topic Emerging Trends and Advances in the Socioeconomic Applications of Beneficial Microbes View all 8 articles

A new strain of Rhodococcus indonesiensis T22.7.1T and its functional potential for deacetylation of chitin and chitooligsaccharides

Provisionally accepted
Junjie Xie Junjie Xie 1,2Doudou Yin Doudou Yin 1,2*Junchao Ou Junchao Ou 1,2*Bo Lu Bo Lu 3*Siming Liao Siming Liao 3*Dengfeng Yang Dengfeng Yang 3*Hongyan Zhang Hongyan Zhang 1,2*Naikun Shen Naikun Shen 1,2*
  • 1 Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning, Guangxi Zhuang Region, China
  • 2 College of Ocean and Biotechnology, Guangxi University for Nationalities, Nanning, Guangxi Zhuang Region, China
  • 3 Guangxi Academy of Sciences, Nanning, Guangxi Zhuang Region, China

The final, formatted version of the article will be published soon.

    Chitin is abundant in marine environments but is challenging to transform and utilize. A strain, T22.7.1T, with chitin deacetylation capabilities was isolated from the rhizosphere of Acanthus ebracteatus in the North Sea of China. Comparative 16S rDNA sequence analysis revealed that the new isolate had the highest sequence similarity (99.79%) with Rhodococcus indonesiensis CSLK01-03T, followed by R. ruber DSM 43338T, R. electrodiphilus JC435T, and R. aetherivorans 10bc312T (98.97%, 98.81%, and 98.83%, respectively). Further genome sequencing and phylogenetic analysis revealed that strain T22.7.1T is an R. indonesiensis species. However, based on additional taxonomic characterization, strain T22.7.1T is recognized as a novel type strain of R. indonesiensis different from CSLK01-03T. Following the first report of this new species in January 2024, this paper refines the taxonomic description of R. indonesiensis and investigates its application in converting chitin into chitosan. Through optimization, the chitin deacetylase (RiCDA) activity of strain T22.7.1T reached 287.02 U/mL, 34.88 times greater than that of the original enzyme (8.0 U/mL). Subsequently, the natural CDA enzyme was isolated and purified from the fermentation products with a purification factor of 31.83, and the specific activity of the enzyme solution reached 1200.33 U/mg. RiCDA demonstrated good pH and temperature adaptability and stability, as well as a wide range of substrate adaptabilities, with deacetylation effects on chitin, chitooligosaccharides, N-acetylglucosamine, etc. Product analysis showed that RiCDA treatment increased the deacetylation degree (DD) of natural chitin to 83%, which was superior to that of commercial chitosan. Therefore, RiCDA, as an excellent deacetylation tool for natural chitin and chitooligosaccharides, has great potential for application in the biorefining of natural polysaccharides.

    Keywords: Rhodococcus indonesiensis, chitin deacetylase, Fermentation optimization, enzyme activity, Chitin oligosaccharides

    Received: 03 May 2024; Accepted: 12 Jul 2024.

    Copyright: © 2024 Xie, Yin, Ou, Lu, Liao, Yang, Zhang and Shen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Doudou Yin, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning, Guangxi Zhuang Region, China
    Junchao Ou, College of Ocean and Biotechnology, Guangxi University for Nationalities, Nanning, Guangxi Zhuang Region, China
    Bo Lu, Guangxi Academy of Sciences, Nanning, Guangxi Zhuang Region, China
    Siming Liao, Guangxi Academy of Sciences, Nanning, Guangxi Zhuang Region, China
    Dengfeng Yang, Guangxi Academy of Sciences, Nanning, Guangxi Zhuang Region, China
    Hongyan Zhang, College of Ocean and Biotechnology, Guangxi University for Nationalities, Nanning, Guangxi Zhuang Region, China
    Naikun Shen, College of Ocean and Biotechnology, Guangxi University for Nationalities, Nanning, Guangxi Zhuang Region, China

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