AUTHOR=di Stefano Giorgia , Battistuzzi Mariano , La Rocca Nicoletta , Selinger Vera M. , Nürnberg Dennis J. , Billi Daniela 

TITLE=Far-red light photoacclimation in a desert Chroococcidiopsis strain with a reduced FaRLiP gene cluster and expression of its chlorophyll f synthase in space-resistant isolates

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 15 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1450575

DOI=10.3389/fmicb.2024.1450575

ISSN=1664-302X

ABSTRACT=<sec><title>Introduction</title><p>Some cyanobacteria can use far-red light (FRL) to drive oxygenic photosynthesis, a phenomenon known as Far-Red Light Photoacclimation (FaRLiP). It can expand photosynthetically active radiation beyond the visible light (VL) range. Therefore, it holds promise for biotechnological applications and may prove useful for the future human exploration of outer space. Typically, FaRLiP relies on a cluster of ~20 genes, encoding paralogs of the standard photosynthetic machinery. One of them, a highly divergent D1 gene known as <italic>chlF</italic> (or <italic>psbA4</italic>), is the synthase responsible for the formation of the FRL-absorbing chlorophyll <italic>f</italic> (Chl <italic>f</italic>) that is essential for FaRLiP. The minimum gene set required for this phenotype is unclear. The desert cyanobacterium <italic>Chroococcidiopsis</italic> sp. CCMEE 010 is unusual in being capable of FaRLiP with a reduced gene cluster (15 genes), and it lacks most of the genes encoding FR-Photosystem I.</p></sec><sec><title>Methods</title><p>Here we investigated whether the reduced gene cluster of <italic>Chroococcidiopsis</italic> sp. CCMEE 010 is transcriptionally regulated by FRL and characterized the spectral changes that occur during the FaRLiP response of <italic>Chroococcidiopsis</italic> sp. CCMEE 010. In addition, the heterologous expression of the Chl <italic>f</italic> synthase from CCMEE 010 was attempted in three closely related desert strains of <italic>Chroococcidiopsis</italic>.</p></sec><sec><title>Results</title><p>All 15 genes of the FaRLiP cluster were preferentially expressed under FRL, accompanied by a progressive red-shift of the photosynthetic absorption spectrum. The Chl <italic>f</italic> synthase from CCMEE 010 was successfully expressed in two desert strains of <italic>Chroococcidiopsis</italic> and transformants could be selected in both VL and FRL.</p></sec><sec><title>Discussion</title><p>In <italic>Chroococcidiopsis</italic> sp. CCME 010, all the far-red genes of the unusually reduced FaRLiP cluster, are transcriptionally regulated by FRL and two closely related desert strains heterologously expressing the chlF010 gene could grow in FRL. Since the transformation hosts had been reported to survive outer space conditions, such an achievement lays the foundation toward novel cyanobacteria-based technologies to support human space exploration.</p></sec>