AUTHOR=Lu Feifei , Lu Guoxiu , Wang Zhiguo , Wei Wenzhua , Chen Jingjing , Zheng Hailin , Liu Xuesong , Ye Yan , Liu Shuling , Lin Yuxiang , Liu Yuxin , Bi Zhanhua , Zou Quanming , Zhang Guoxu , Sun Hongwu , Tong Yanan 

TITLE=Improved antibacterial activity of a sustained-release biocompatible nanofilm for treating Pseudomonas aeruginosa wound infection in vitro and in vivo

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1548106

DOI=10.3389/fmicb.2025.1548106

ISSN=1664-302X

ABSTRACT=ObjectivesThe primary goal of this research was to design a useful and biocompatible nanofilm system (CNF) encapsulating chlorhexidine acetate (CHX) for wounds that is endowed with antibacterial and anti-inflammatory activities and promotes wound healing.MethodsThe nanofilm system was developed on the basis of the successful preparation of its nanoemulsion and PVA-CS film system and then important properties of the nanofilm system, including its morphological and physicochemical characteristics, stability and safety, its antimicrobial efficacy against P. aeruginosa was also evaluated in vitro and in vivo. The antibacterial effect, wound healing effect and inflammatory factor change in vivo were evaluated.ResultsThese results of this nanofilm system revealed a good particle size (59.27 nm) and stable zeta potential (−15.2 mV) that are suitable for wound healing applications. Additionally, it was stable, exhibited long-term stability (24 months) and sustained release in simulated wound fluid. Results showed that this nanofilm does not induce dose-related toxic effects and displays a better antibacterial effect that occurs more quickly, two times greater than that of CHX in vitro. This safe nanofilm enhances antibacterial activity against P. aeruginosa for 14 days, modulates the immune response, and accelerates skin wound healing in vivo.ConclusionsThese insights into multifunctional nanofilm designs for improved antibacterial effects and sustained release suggest promising clinical applications.