AUTHOR=Khan Sajjad Ahmed , Rather Muzafar Ahmad , Sheeraz Ahmad Muhammad , Jia Ziyi , Baughn Anthony D. , Iqbal Sajid , Qadir Syed Mehmood , Tahseen Sabira , Khan Muhammad Umer , Villalta Peter W. , Shier W. Thomas 

TITLE=Identification of Datura innoxia as a potential source of antimycobacterial components

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1553282

DOI=10.3389/fmicb.2025.1553282

ISSN=1664-302X

ABSTRACT=Datura innoxia is a medicinal plant from the Solanaceae family, having medicinal properties and some toxic effects. It is widely distributed across Asia, Africa, Europe, the Americas, and other tropical and subtropical regions, where it is utilized by local pharmaceutical industries. In this study, bioassay-guided fractionation and LC-MS/MS analysis were used for the identification of secondary metabolites with anti-tuberculosis activity in methanolic leaf extracts of D. innoxia. Bioassay-guided fractionation was conducted using normal and reverse phase column chromatography, and the fractions were assayed for antituberculosis activity in vitro by serial dilution in Mycobacterium tuberculosis H37Ra cultures. The structures of known secondary metabolites in the purified extracts were identified using LC-ESI-MS/MS mass spectroscopy. A purified fraction of the methanolic extract of D. innoxia leaves inhibited M. tuberculosis growth at concentrations as low as 25 μg/mL. Metabolic profiling with LC-ESI-MS/MS enabled the identification of the purified extract of 16 known metabolites, including loliolide, scopolamine, kuromanin, isoquercitrin, moupinamide, methyl isoquinoline-3-carboxylate, trans-3-Indoleacrylic acid, tyramine, (3β,5ξ,9ξ)-3,6,19-trihydroxyurs-12-en-28-oic acid, milbemycin A3 oxime, methyl jasmonate, nicotinamide, methyl ferulate, trifolin, 2-[(1S,2S,4aR,8aS)-1-hydroxy-4a-methyl-8-methylidene-decahydronaphthalen-2-yl]prop-2-enoic acid, and methyl 4-hydroxycinnamate. These results indicate that D. innoxia is a rich natural source of potential antitubercular secondary metabolites.