AUTHOR=Díaz-Navarro Marta , Irigoyen-von-Sierakowski Álvaro , Delcán Imani , Monte Ariadna , Palomo María , Escribano Pilar , Guinea Jesús , Burillo Almudena , Galar Alicia , Muñoz Patricia , Guembe María 

TITLE=New insights in the role of Candida biofilm in the pathogenesis of recurrent vulvovaginal candidiasis: a prospective clinical study

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1566171

DOI=10.3389/fmicb.2025.1566171

ISSN=1664-302X

ABSTRACT=BackgroundDespite the pathogenesis of vulvovaginal candidiasis (VVC) is multifactorial, this study aimed to assess whether phenotypic characteristics, such as biofilm production and quality, along with clinical symptoms, are associated with recurrent VVC (RVVC).MethodsOver 1 year (Oct 2021–Oct 2022), we prospectively included 271 patients ≥18 years who attended our institution, had Candida spp. isolated in vaginal swabs, and provided informed consent. Patients were followed for 1 year. Candida spp. isolates were tested by the following techniques: crystal violet (CV) for biomass quantification, XTT for metabolic activity quantification, and microscopy for biofilm area quantification. Clinical and microbiological data were also collected.ResultsOverall, 55 (20.3%) patients experienced at least one recurrence, with 19 (7.0%) meeting the criteria for RVVC (≥3 episodes/year), with 65 episodes in total. Demographic and clinical characteristics were similar in both study groups. Most isolates were C. albicans (90.0%). Median (interquartile, [IQR]) absorbance values for CV and XTT in 18/19 RVVC and 238/252 non-RVVC isolates were as follows: CV, 1.850 (1.578–2.156) vs. 1.426 (1.081–1.823), p = 0.005; XTT, 0.184 (0.116–0.293) vs. 0.228 (0.147–0.331), p = 0.253. Median (IQR) biofilm occupation area percentage in 16/19 RVVC and 16/252 non-RVVC isolates was, respectively: 13.15 (8.54–16.9) and 10.73 (5.88–17.73), p = 0.710.ConclusionRVVC was associated to high biomass production. Additionally, RVVC clinical isolates exhibited a tendency toward lower metabolic activity, which may contribute to treatment failure.