AUTHOR=Sunthornthummas Sirinthorn , Wasitthankasem Rujipat , Phokhaphan Pimonwan , Sudtachat Nirinya , Wilantho Alisa , Ngamphiw Chumpol , Chareanchim Wanwisa , Tongsima Sissades TITLE=Unveiling the impact of 16S rRNA gene intergenomic variation on primer design and gut microbiome profiling JOURNAL=Frontiers in Microbiology VOLUME=Volume 16 - 2025 YEAR=2025 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1573920 DOI=10.3389/fmicb.2025.1573920 ISSN=1664-302X