AUTHOR=Alanazi Yasmene F. , Alrdahe Salma Saleh , Moussa Zeiad , Darwish Doaa Bahaa Eldin , Alrdahi Haifa , Saber WesamEldin I. A. TITLE=Endophytic Trichoderma asperellum WNZ-21 produces novel bioactives with anticancer, anti-inflammatory, and hemoprotective properties from fermented bean biomass JOURNAL=Frontiers in Microbiology VOLUME=Volume 16 - 2025 YEAR=2025 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1609361 DOI=10.3389/fmicb.2025.1609361 ISSN=1664-302X ABSTRACT=The increasing demand for novel bioactive compounds is driving research into natural sources, particularly through the valorization of agricultural residues. Endophytic fungi have emerged as a promising source of bioactive metabolites with diverse therapeutic potential. We employed a novel approach utilizing the isolated Trichoderma asperellum WNZ-21 [identified morphologically and molecularly (ITS: OR857252, tef1 gene: PP069312)] in semi-solid-state fermentation to bioconvert common bean biomass residue (RCBB) into a rich source of metabolites with significant therapeutic potential. First, T. asperellum efficiently degraded 67.19% RCBB, exhibiting robust enzymatic activity (cellulase: 18.067 units per gram (U/g), xylanase: 15.967 U/g, protease: 5.550 U/g). The fermented RCBB filtrate exhibited a rich chemical profile, including (mg/g RCBB) amino acids (13.51), phenolics (55.22), flavonoids (11.22), tannins (18.90), and several secondary metabolites. FT-IR analysis indicated the presence of a complex mixture of amino acids, polysaccharides, proteins, and other metabolites. GC-MS analysis identified 17 compounds. The fermented biomass possesses multi-biological activities, including anticancer against hepatocellular carcinoma (IC50 = 35.62 μg/mL) and mammary gland breast cancer (IC50 = 59.20 μg/mL) cell lines, with reduced toxicity toward normal human lung fibroblast cells (IC50 = 76.33 μg/mL). It also exhibits anti-hemolytic activity in erythrocyte protection assays, reducing oxidative stress-induced hemolysis to 4.40%. Additionally, the filtrate demonstrates anti-inflammatory activity in a carrageenan-induced paw edema model in rats, providing 36.50% protection after 5 h of treatment. Our findings establish RCBB valorization as a promising route to produce multi-bioactive fungal metabolites. Future research should focus on isolating active compounds, optimizing production, and conducting in vivo studies to fully realize therapeutic potential.