AUTHOR=Zhang Huilin , Chen Huiyang , He Chaoyue , Li Wenchao , Li Falei TITLE=Distribution of human-pathogenic Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in crab-eating macaques in China JOURNAL=Frontiers in Microbiology VOLUME=Volume 16 - 2025 YEAR=2025 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1641632 DOI=10.3389/fmicb.2025.1641632 ISSN=1664-302X ABSTRACT=IntroductionThe positive rates and genetic identity of Cryptosporidium spp., Giardia duodenalis (G. duodenalis), and Enterocytozoon bieneusi (E. bieneusi) were unclear in crab-eating macaques in Suzhou and Beijing, China.MethodsA total of 504 fecal samples were collected from crab-eating macaques on commercial farms in Beijing and Suzhou, China. The extracted DNA was analyzed for Cryptosporidium spp. and E. bieneusi by nested PCR and sequence analysis of the small subunit rRNA (SSU rRNA) gene and the internal transcribed spacer (ITS) gene, respectively. The G. duodenalis was detected by nested PCR targeting β-giardin (bg) gene, glutamate dehydrogenase (gdh) gene, and triosephosphate isomerase (tpi) gene. The C. hominis identified were further subtyped by nested PCR and sequence analysis of the 60 kDa glycoprotein (gp60) gene.ResultsAll 504 fecal samples collected from crab-eating macaques, the detection rates of Cryptosporidium spp., G. duodenalis, and E. bieneusi were 11.9% (60/504), 5.6% (28/504), and 4.6% (23/504), respectively. The 15.1% (44/292) detection rate of Cryptosporidium spp. from crab-eating macaques in Suzhou was significantly higher than that in Beijing (2.8%; 6/212; χ2 = 20.6, df = 1, p < 0.0001). The detection rates of Cryptosporidium spp. and G. duodenalis were significant different between <2 months old animals and >24 months old animals (χ2 = 104.7, df = 1, p < 0.0001; χ2 = 6.6, df = 1, p = 0.0104). In contrast, there was no significant different in the detection rate of E. bieneusi in two age groups (χ2 = 2.2, df = 1, p = 0.1360). A total of one Cryptosporidium species, one G. duodenalis assemblage B, and 4 E. bieneusi genotypes have been identified, including C. hominis (n = 60), assemblage B (n = 28), CM1 (n = 14), Peru8 (n = 5), D (n = 3), and Type IV (n = 1). Among 60 C. hominis samples, five subtypes of five subtype families were successfully identified at the gp60 gene: IbA13G4 (n = 27), InA26 (n = 3), IfA17G2R3 (n = 3), IiA17 (n = 3), and IeA11G3T3 (n = 2).DiscussionThe results indicate that known zoonotic Cryptosporidium spp., G. duodenalis, and E. bieneusi are prevalent in crab-eating macaques. The crab-eating macaques could play a potential role in the zoonotic transmission of pathogens to humans.