AUTHOR=Zhang Xinyue , Guan Lingfeng , Xu Zhenghong , Wang Hanchuan , Wei Xinyan , Yang Min , Qin Qiwei , Wang Shaowen 

TITLE=Aptamer based lateral flow biosensor for rapid detection of largemouth bass virus

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1643764

DOI=10.3389/fmicb.2025.1643764

ISSN=1664-302X

ABSTRACT=The disease caused by Largemouth bass virus (LMBV) is one of the most serious viral diseases that threaten the development of largemouth bass industry. Point-of-Care testing of LMBV infection is crucial to prevent and control disease. Here, we designed a simple and sensitive aptamer-based lateral flow biosensor (LFB) method for the rapid detection of LMBV. In this method, two different aptamers (LA38s and LA13s) specific for LMBV particles were used for target capture and initiation of isothermal strand displacement amplification (SDA), respectively. After adding the reaction product to LFB, the color change of the control zone (C-line) and the test zone (T-line) can be observed by naked eyes within 5 min. The LFB possesses high specificity and accuracy for LMBV detection in vivo and in vitro. The detection limit of LFB is as low as 8 × 101/mL LMBV infected cells, with the detection time of 1 h. Thus, this novel LFB provides a new detection tool, which can be used for rapid detection of LMBV in field.