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        <title>Frontiers in Microbiomes | New and Recent Articles</title>
        <link>https://www.frontiersin.org/journals/microbiomes</link>
        <description>RSS Feed for Frontiers in Microbiomes | New and Recent Articles</description>
        <language>en-us</language>
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        <pubDate>2026-06-05T18:00:13.671+00:00</pubDate>
        <ttl>60</ttl>
        <item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1849762</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1849762</link>
        <title><![CDATA[Fecal microbiota transplantation promotes gut microbiome recovery in pediatric hematopoietic stem cell transplant recipients]]></title>
        <pubdate>2026-06-05T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>María Florencia Fernandez</author><author>Abigail Stricker</author><author>Adriana Bottero</author><author>Laura Busquet</author><author>Carlos Waldbaum</author><author>Fabiana López Mingorance</author><author>Raúl Martinez Patetta</author><author>Ignacio Toer</author><author>Ana Juliá</author><author>Andrea Mangano</author>
        <description><![CDATA[IntroductionHematopoietic stem cell transplantation (HSCT) profoundly disrupts the gut microbiome and may contribute to adverse post-transplant outcomes. Fecal microbiota transplantation (FMT) has emerged as a strategy to restore microbial diversity; however, data in pediatric HSCT recipients remain limited.MethodsWe conducted a longitudinal analysis of 17 pediatric HSCT recipients who received FMT. Fecal samples were collected before FMT and at days 7, 14, and 30 after treatment. Gut microbiome composition was analyzed using 16S rRNA gene sequencing.ResultsBaseline samples showed reduced microbial diversity and a dysbiotic microbial profile. Following FMT, microbial diversity increased progressively, with recovery evident from day 7 and stabilization by day 30. Taxonomic analyses demonstrated depletion of dysbiosis-associated genera and enrichment of beneficial short-chain fatty acid–producing taxa, including Faecalibacterium, Blautia, Subdoligranulum, and Akkermansia. Distinct microbial configurations were observed according to gastrointestinal involvement by acute graft-versus-host disease.ConclusionsFMT was associated with progressive restoration of gut microbiome diversity and structure in pediatric HSCT recipients, supporting its potential role as a microbiota-based strategy to promote ecological recovery after HSCT.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1804117</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1804117</link>
        <title><![CDATA[Age-specific early-life gut microbiome associations with eczema and food allergies during early immune development]]></title>
        <pubdate>2026-05-29T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Harold Nunez</author><author>Timothy J. Straub</author><author>Nabeel Imam</author><author>David Goad</author><author>Noel T. Mueller</author><author>Ruben A. T. Mars</author><author>Cheryl Sew Hoy</author><author>Trillitye Paullin</author><author>Kimberley V. Sukhum</author>
        <description><![CDATA[IntroductionEczema and food allergy commonly emerge during infancy and are linked to changes in the gut microbiome, yet it remains unclear when microbiome differences associated with allergic disease first appear during development.MethodsWe analyzed age-stratified shotgun metagenomic data from 97 children aged 4–36 months, including physician-confirmed cases of eczema or food allergy and non-allergic controls, excluding recent antibiotic or probiotic exposure. Microbial taxa, functional pathways, and composite microbiome metrics were evaluated across three developmental stages: early infancy (4–6 months), mid-infancy (6–12 months), and toddlerhood (12–36 months).ResultsDifferences between allergic and non-allergic children were minimal before 6 months of age but became more apparent during mid-infancy and persisted into toddlerhood. Allergic conditions were associated with reduced abundance of fiber-fermenting and butyrate-producing taxa, enrichment of facultative and inflammation-associated microbes, lower microbiome maturation scores, and shifts in metabolic and inflammatory functional capacity.DiscussionThese findings suggest that gut microbiome divergence associated with allergic disease becomes more apparent during mid-infancy, highlighting a developmentally relevant period for understanding early immune disruption. The results support further longitudinal and interventional studies aimed at clarifying whether earlier microbiome-targeted strategies may help modify progression along the atopic march.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1785707</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1785707</link>
        <title><![CDATA[Altered early-life gut microbiota in offspring of pregnancies complicated by CHD-associated pulmonary hypertension]]></title>
        <pubdate>2026-05-29T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Yiyang Han</author><author>Haofeng Zhang</author><author>Jun Zhang</author>
        <description><![CDATA[BackgroundPulmonary arterial hypertension is a progressive disease involving the pulmonary vasculature and is defined as a mean pulmonary arterial pressure (mPAP) >20 mmHg at rest. Pulmonary arterial hypertension during pregnancy is associated with increased maternal mortality and adverse fetal outcomes. The present study aimed to investigate differences in the initial meconium microbiota between neonates born to mothers with congenital heart disease-associated pulmonary arterial hypertension (CHD-PAH) and those born to mothers with congenital heart disease (CHD) alone, thereby elucidating the potential influence of pulmonary arterial hypertension on the establishment of the early-life gut microbiome.MethodsWe collected first-pass meconium samples from neonates in the pulmonary hypertension group (PH group, n = 23) and the control group without pulmonary hypertension (NC group, n = 17) and characterized microbial profiles using 16S rRNA sequencing.ResultsThe PH group showed lower alpha diversity, with reduced Shannon and observed features indices (both P < 0.05), whereas Bray–Curtis beta diversity showed substantial overlap between groups. At the phylum level, the overall gut microbial structure was broadly comparable between the PH and NC groups, with no statistically significant differences in the relative abundance of dominant taxa. At the genus level, the mean relative abundance of Streptococcus was significantly lower in the PH group than in the NC group (0.20% vs. 2.09%, P = 0.0072). Predicted functional profiling suggested potential differences in dominant metabolic pathways between groups, including enrichment of ubiquinone biosynthesis and aromatic amino acid/chorismate biosynthesis pathways in the PH group.ConclusionCollectively, these findings extend current evidence on PAH-related alterations in early-life microbial ecosystems and provide a plausible microbiome-based basis for investigating the biological mechanisms underlying adverse maternal–fetal outcomes associated with pulmonary arterial hypertension.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1774429</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1774429</link>
        <title><![CDATA[The interplay between bile acid metabolism and gut microbiome in biliary tract cancers]]></title>
        <pubdate>2026-05-28T00:00:00Z</pubdate>
        <category>Review</category>
        <author>Ifeoma Ike</author><author>Farzad Teymouri</author><author>Christiana Crook</author><author>Sofia Guzman</author><author>Max Hazeltine</author><author>Dani Castillo</author><author>Daneng Li</author><author>Gagandeep Brar</author>
        <description><![CDATA[The gut microbiota and bile acids (BAs) exist in a tightly regulated, bidirectional relationship that influences host metabolism, immune function, and disease. Primary BAs synthesized in the liver are chemically transformed by intestinal microbes into a diverse pool of secondary BAs, which exert antimicrobial effects and activate host signaling pathways including Farnesoid X Receptor (FXR), Takeda G protein–coupled receptor 5 (TGR5), and sphingosine-1-phosphate receptor 2 (S1PR2). These pathways regulate BA homeostasis, epithelial barrier integrity, inflammation, and carcinogenesis. Disruption of this BA–microbiome axis has been implicated in biliary tract cancers (BTCs), a group of aggressive malignancies with rising global incidence and limited therapeutic options. Secondary BAs and BA receptor signaling contribute to tumor initiation and progression through NF-κB activation, oxidative stress, and altered cell survival, whereas reduced FXR signaling and obstructed enterohepatic circulation further promote inflammatory dysregulation. Emerging evidence demonstrates that microbial dysbiosis and altered BA metabolism are associated with distinct BTC microbial profiles, enriched in taxa such as Fusobacterium, Salmonella, Prevotella, and Actinomyces, alongside depletion of commensals including Lactobacillus. These taxa influence inflammatory signaling, BA transformation, and epithelial injury, contributing to carcinogenesis. Microbiome–BA interactions also shape anti-tumor immunity and responses to immune checkpoint inhibitors (ICIs). Specific microbial signatures—particularly enrichment of Lachnospiraceae, Erysipelotrichaceae, Bacteroidetes, and Alistipes—correlate with enhanced immune activation and improved clinical outcomes in hepatobiliary cancers. Modulation of gut microbiota through antibiotics, probiotics, or fecal microbiota transplantation can influence BA composition, immune surveillance, and therapeutic efficacy. Collectively, these data highlight the central role of the BA–microbiome axis in BTC pathogenesis and treatment response. Microbial and BA metabolite profiling represent promising avenues for biomarker development, while targeted manipulation of BA signaling and microbial ecology offers potential therapeutic strategies to improve BTC outcomes.]]></description>
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        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1808609</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1808609</link>
        <title><![CDATA[Soil microbial communities shift in response to cropping sequence diversification with perennial seed crops]]></title>
        <pubdate>2026-05-26T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Newton Z. Lupwayi</author><author>Nityananda Khanal</author><author>Mathew Richards</author><author>Rodrigo Ortega Polo</author>
        <description><![CDATA[Integrating perennial forage seed crops into annual cropping sequences can diversify the rotations and improve soil health, yet their effects on the soil microbial communities and functions are not yet fully elucidated on the Canadian prairies. Using a 10-year field experiment with eight cropping sequences under varying supplemental nitrogen (N) fertilization levels, we evaluated the impacts of integrating perennial seed crops and annual crops on soil microbial biomass carbon (MBC), the composition and diversity of prokaryotic and fungal communities, and the activities of key enzymes involved in carbon (C), N, phosphorus (P), and sulfur (S) cycling, namely β-glucosidase, N-acetyl-β-glucosaminidase, acid phosphomonoesterase and arylsulfatase. The crop sequences containing intermittent succession of perennial and annual crops had 17% greater soil MBC, higher fungal richness (e.g., Chao1 indices of 92.8 vs. 87.6) and 22% greater β-glucosidase activity than annual-only sequences. The relative abundances of the two most abundant prokaryotic phyla - Actinobacteriota and Proteobacteria - as well as the second most abundant fungal class, Dothideomycetes, followed the same trend. The soils with more frequent recurrence of grassy perennials in the sequences exhibited greater MBC (34%), higher prokaryotic Shannon diversity, greater fungal richness, and higher arylsulfatase activity (68%) than soils with more frequent recurrence of perennial legumes, although the predominant prokaryotic phylum, Actinobacteriota was more abundant in legume-based systems. The cropping sequences dominated by creeping red fescue grass seed crops exhibited the greatest improvement in most of the soil microbial metrics studied. Nitrogen fertilizer increased the relative abundance of the copiotrophic Actinobacteriota but decreased that of the oligotrophic Acidobacteriota. Prokaryotes were associated with C, N, P and S cycling, whereas fungi were primarily linked to C cycling. Overall, diversifying annual grain cropping systems with perennial forage seed crops, particularly creeping red fescue, enhanced key indicators of biological soil health.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1832705</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1832705</link>
        <title><![CDATA[Dietary transition to an Indigenous Greenlandic diet induces instant shifts in gut microbiota composition – a pilot intervention study]]></title>
        <pubdate>2026-05-21T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Mads B. W. Bjørnsen</author><author>Katie M. Bourke</author><author>Catherine Stanton</author><author>R. Paul Ross</author><author>Anders J. Hansen</author><author>Aviaja L. Hauptmann</author>
        <description><![CDATA[IntroductionNon-Western diets are increasingly studied for their relationship to gut microbiota composition and diversity, although most research in this area has focused on plant-based, fiber-rich diets. Here, we present a single-participant longitudinal study investigating gut microbiota dynamics during a transition from a Western diet to a 12-week Indigenous Arctic animal-based diet composed of minimally processed raw, dried, and fermented animal-source foods. During one month of this period, the participant consumed dried whole fish (ammassak), including intestinal contents, representing a form of gastrophagy, a practice common to the Arctic diet, that may increase exposure to food-associated microbes.MethodsFecal samples (n = 29) were collected before, during, and after the Arctic diet phase. 16S rRNA gene sequencing of the V3-V4 region was used to profile bacterial communities. Diversity metrics, Firmicutes/Bacteroidota (F/B) ratios, and taxonomic composition analyses were performed to assess compositional shifts across diet phases.ResultsAlpha diversity remained relatively steady throughout the study, with a tendency toward higher values during the Arctic diet. The F/B ratio increased from 1.31 to 2.12 during the Arctic diet phase and remained elevated (2.38) after returning to a Western diet. Beta diversity analysis revealed significant restructuring of the gut microbiota at the onset of the Arctic diet, followed by partial reversibility upon returning to a Western diet. Fiber-associated taxa like Prevotella 9 disappeared, and Bifidobacterium declined, while protein- and fat-associated taxa, including Bacteroides, Lachnoclostridium, and Alistipes, increased. Several genera appeared during the Arctic diet phase that were absent during the preceding Western diet phase, consistent with altered microbial exposure. Among those, Photobacterium was also detected in the ammassak, suggesting potential microbial exposure during the gastrophagy period.DiscussionThese results provide preliminary evidence that the gut microbiota can shift substantially during an Indigenous Arctic dietary transition. Because the Arctic diet also substantially overlapped with sustained high physical activity, the observed changes should be interpreted in the context of a combined dietary and lifestyle transition. These findings highlight the need for a better understanding of underrepresented dietary patterns, such as those of Arctic Indigenous communities, and their relationship with the gut microbiota.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1842701</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1842701</link>
        <title><![CDATA[Computational and multi-omics systems biology for precision microbiome therapeutics]]></title>
        <pubdate>2026-05-15T00:00:00Z</pubdate>
        <category>Mini Review</category>
        <author>Ahmed Dewan</author><author>Maria Teresa Mascellino</author>
        <description><![CDATA[The human gut microbiome represents a complex and dynamic therapeutic target whose effective interrogation requires system-level analytical approaches beyond single-omics or reductive methods. This mini-review synthesizes recent advances in computational modeling and multi-omics integration relevant to the development of predictive, patient-tailored microbiome therapies. We critically assess the analytical strengths and limitations of genome-scale metabolic models (GEMs); generalized Lotka–Volterra and ODE-based community models; agent-based simulations; and statistical machine-learning frameworks and examine how their integration with metagenomics, metatranscriptomics, metaproteomics, and metabolomics can help bridge microbial functional potential with clinically relevant phenotypes. Representative applications–including MintTea for disease module identification, gNOMO2 for integrative microbiome profiling, and AGORA-based community metabolic modeling–illustrate the translational scope of these frameworks across inflammatory, metabolic, and infectious disease contexts. Hybrid ML–GEM frameworks have not yet been directly applied to FMT outcome prediction; however, the mechanistic principles underlying both approaches – metabolic compatibility modeling and data-driven responder stratification – suggest a compelling direction for future investigation, contingent on prospective validation in adequately powered and independent clinical cohorts. Persistent methodological challenges–such as data heterogeneity, batch effects across sequencing platforms, incomplete multi-omics coverage, and limited interpretability of complex machine-learning models–are being actively addressed through standardized preprocessing pipelines, explainable Artificial intelligence (AI) strategies, and federated analytics. While federated approaches enable privacy-preserving, multi-institutional model training, they introduce additional constraints related to non-identically distributed data, communication overhead, and uneven computational capacity. Overall, the convergence of mechanistic modeling, data-driven learning, and distributed analytical infrastructures may assist in advancing microbiome research from a largely correlational perspective toward mechanistic and ultimately prescriptive frameworks for precision microbiome medicine.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1778537</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1778537</link>
        <title><![CDATA[Host genotype and environment shape rhizosphere and root microbiome composition of pecan rootstocks]]></title>
        <pubdate>2026-05-15T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Paul Oladimeji Gabriel</author><author>Ciro Velasco-Cruz</author><author>Jennifer J. Randall</author>
        <description><![CDATA[The rhizosphere and root-associated microbiomes play a crucial role in nutrient acquisition, stress tolerance, and overall plant performance. However, little is known about how microbial communities assemble and shift across environments in pecan (Carya illinoinensis). In this study, we compared the bacterial and fungal community compositions in the roots and rhizosphere of four pecan clonal rootstocks (NMU03, NMU04, NMU05, and NMU155) cultivated under greenhouse conditions, as well as their subsets that were subsequently transplanted to the field. Amplicon sequencing of 16S rRNA and ITS regions revealed significant differences in microbial diversity and taxonomic composition across environments and genotypes. Bacterial assemblages in greenhouse roots were typically dominated by a few families (e.g., Burkholderiaceae, Rhodanobacteraceae, and unclassified taxa). In contrast, field samples exhibited broader taxonomic distributions, with families such as Xanthobacteraceae, Haliangiaceae, and Geminicoccaceae emerging as dominant members. Fungal OTU abundance was consistently higher than bacterial abundance across all genotypes, likely reflecting mutualistic associations with mycorrhizal fungi, such as those in the Elaphomycetaceae family. Interestingly, Aspergillaceae dominated greenhouse and field fungal communities, suggesting ecological adaptability and potential contributions to plant stress tolerance. Comparisons with earlier greenhouse studies revealed that while some signature core microbiome members were retained following transplantation from the greenhouse to the field, the abundance of others decreased, highlighting successional shifts in community structure driven by environmental transitions. Together, these findings demonstrate the dynamic, genotype and environment-specific structuring of pecan microbiomes and highlight the importance of microbiome-informed breeding strategies to improve plant-microbe associations under variable growth conditions among pecan breeders.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1803341</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1803341</link>
        <title><![CDATA[Protist community sites and structure under two barn management systems at a commercial dairy]]></title>
        <pubdate>2026-05-14T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Tawni L. Crippen</author><author>Dongmin Kim</author><author>Sonja L. Swiger</author><author>Robin C. Anderson</author>
        <description><![CDATA[IntroductionInvestigations into the location and load of protists in the environment arounddairies are scarce but are essential to maintaining the health of livestock.Moreover, the design of dairy barns has fluctuated over the decades to maximizecattle health and milk production without regard to influences on environmentalmicrobiomes. Beyond cost, the major emphasis of barn design is the managementof appropriate temperature and comfort for cattle. However, there havebeen no corresponding investigations into whether these design changes affect protist communities within barns.MethodsIn this study, community shotgun metagenomic analysis was used to define the spatial composition and relative abundance of protist communities from 118 samples of manure, lagoons, troughs, and house and stable flies at a commercial dairy implementing two free-stall management systems: flow-through and cross-vent. Sequence reads were mapped to the CosmosID database. Viability was not assessed; therefore, results reflect DNA detection only not viability or disease occurrence.ResultsThe protist composition differed significantly between dairy components. Ecological findings showed that troughs and lagoons harbored high protist diversity, including the possible pathogen Neobalantidium coli and potential carriers Paramecium biaurelia and Acanthamoeba. Manure had the lowest protist diversity. Stable flies carried more protist taxa than house flies. Both fly species uniquely carried the non-pathogenic alveolate parasite Hammondia hammondi. The water mold plant pathogen Pseudoperonospora cubensis was identified in all sample types. Of the total relative abundance of protists, 2.10% were amoebas, 7.63% alveolate parasites, 62.71% water molds, 23.31% ciliates, 1.74% foraminifera, and 2.50% diatoms.DiscussionThese results describe preliminary spatial overlaps and possible avenues of dissemination, providing a basis for assessing appropriate management systems and identifying protist reservoir sites within dairy operations.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1831956</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1831956</link>
        <title><![CDATA[Identifying microbial biomarkers of neurodegeneration: a comparative study in Alzheimer’s and Parkinson’s disease]]></title>
        <pubdate>2026-05-12T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Simon De Jaegher</author><author>David Pinzauti</author><author>Maria D’Aguanno</author><author>Erika Parkinson</author><author>James Schofield</author><author>Fabio Strazzeri</author><author>Paul Skipp</author><author>Rebekah Penrice-Randal</author><author>Amy Kunicki</author><author>Beth McCausland</author><author>Christopher Kipps</author><author>Jay Amin</author><author>Manuele Biazzo</author>
        <description><![CDATA[IntroductionNeurodegenerative disorders such as Alzheimer’s disease (AD) and Parkinson’s disease (PD) have been increasingly linked to alterations of the gut microbiota, although reported microbial signatures remain heterogeneous and often lack taxonomic resolution.MethodsIn the present study, we applied full-length 16S rRNA gene sequencing to characterize gut microbiota composition in 152 individuals, including patients with AD (n = 37), PD (n = 65), and age-matched healthy controls (n = 50), using a unified bioinformatic and statistical framework with adjustment for relevant demographic covariates.ResultsAlzheimer’s disease was associated with a modest but significant reduction in microbial richness and Shannon diversity compared with controls, whereas no alpha diversity differences were observed in PD. Beta diversity analyses revealed significant compositional differences across diagnostic groups, driven primarily by PD and modulated by sex but not age. Species-level differential abundance analysis identified a PD-associated microbial signature characterized by reduced abundances of short-chain fatty acid-producing bacteria, including Faecalibacterium prausnitzii, Agathobacter rectalis, Roseburia intestinalis, and Faecalicatena fissicatena, together with increased abundance of Ruminococcus sp. JE7A12. In contrast, AD exhibited minimal species-level changes, with only Bacteroidales bacterium CF showing reduced abundance compared with controls.DiscussionOverall, these findings indicate that Parkinson’s disease is characterized by a targeted disruption of beneficial butyrate-producing bacteria, whereas Alzheimer’s disease exhibits subtler and less consistent microbiome alterations. Our results underscore the importance of species-level resolution for identifying disease-associated microbial signatures.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1787662</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1787662</link>
        <title><![CDATA[Importance of human microbiome: an update]]></title>
        <pubdate>2026-04-30T00:00:00Z</pubdate>
        <category>Review</category>
        <author>Maryem Wardi</author><author>Abdulmumini Baba Amin</author><author>Imane El Belghiti</author><author>Zohra Lemkhente</author><author>Ahmed Belmouden</author>
        <description><![CDATA[Millions of microorganisms—including bacteria, viruses, fungi, archaea, and protists—reside on and within the human body, collectively forming the human microbiota. This complex and dynamic community plays a crucial role in modulating physiological processes, particularly the development and regulation of the immune system. Modern behaviors such as frequent washing, excessive hygiene, and widespread use of antimicrobial agents can disrupt the natural composition and functional balance of the microbiota, leading to altered immune responses and increased susceptibility to disease. In this review, we focus primarily on the bacterial component of the human microbiome. While we acknowledge the importance of viruses, fungi, archaea, and protists, these components are beyond the scope of the current review. We highlight recent advances in bacterial microbiome research that are reshaping our understanding of host–microbe interactions, immune modulation, and the health consequences of microbiota dysbiosis.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1672092</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1672092</link>
        <title><![CDATA[Characterization of the gut microbiome of wild Peromyscus sonoriensis in New Mexico, USA]]></title>
        <pubdate>2026-04-24T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Andrew M. Skidmore</author><author>Samuel M. Goodfellow</author><author>Robert A. Nofchissey</author><author>Limin Jiang</author><author>Jonathan Dunnum</author><author>Joseph A. Cook</author><author>Yan Guo</author><author>Ivana Mali</author><author>Steven B. Bradfute</author>
        <description><![CDATA[The microbiome is highly important to the physiologies of all multicellular organisms, particularly metazoans. However, the microbiomes of many wild animals remain understudied and poorly understood. Peromyscus mice are commonly used as models of adaptation, mental health, and human disease in biomedical research, and are also common in the environment across North America, frequently coming into close contact with humans. Additionally, Peromyscus sonoriensis are implicated as the primary reservoir for Sin Nombre hantavirus, a rare but severe disease of high morbidity and mortality in humans. Here we characterize the fecal microbiomes of 311 Peromyscus sonoriensis, the western deer mouse, collected from across New Mexico, USA, which will further their usefulness as models of disease and behavior as well as increase our understanding of their ecology. The animals used in this study are geographically diverse, collected from multiple ecoregions, and encompass mice of all ages and sexes. We sequenced the entire 16S rRNA gene in a PCR independent approach and characterized the microbiomes with Shannon entropy, Faith phylodiversity, and weighted UNIFRAC. We found that these mice have diverse microbiomes, with individuals varying in the presence and proportions of various identified bacteria. We analyzed the total population of mice according to age, sex, and trapping location, and found that trapping location was the only condition to significantly impact the microbiome. When the mice were subdivided by the location of collection, there were mild effects of age and sex. When comparing mice from archival museum storage, storage of samples in 95% ethanol resulted in significant alterations to the microbiome when compared to cryopreservation. Differential bacterial family presence was determined using ANCOMBC at the 0.05 significance threshold, and there were many differentially abundant families across all groups of mice. This data set can now be used as a reference for further research into the microbiomes of related Peromyscus species, enhance the use of P. sonoriensis as model laboratory animals, and as a source of novel research questions regarding the physiology of these rodents.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1782119</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1782119</link>
        <title><![CDATA[Uncovering leaf and root microbiomes of mangrove trees in French Guiana]]></title>
        <pubdate>2026-04-22T00:00:00Z</pubdate>
        <category>Brief Research Report</category>
        <author>Mathilde Vigneron</author><author>Sébastien Halary</author><author>Sandrine Crochemore</author><author>Laetitia Plaisance</author><author>Nathalie Parthuisot</author><author>Yvan Bettarel</author>
        <description><![CDATA[Microorganisms are now widely acknowledged as essential contributors to the health and resilience of coastal environments. Yet, mangrove ecosystems, despite offering numerous ecological and economic services, remain relatively overlooked in microbial research. In this study, we examined the bacteriome of the rhizosphere and the phyllosphere of two mangrove tree species: Avicennia germinans and Rhizophora mangle. Both species were sampled along the banks of the Sinnamary estuary in French Guiana. Our results revealed notable differences in microbiome composition between the two organs and between the two tree species. On average, only 0.3% of ASVs were shared between the leaves and roots and 2.2% between A. germinans and R. mangle. The taxonomic differences were characterized mainly by the significant presence of Rhodothermia and Bacteroidia in the leaves and Cyanobacteria and Planctomycetia in the roots. Furthermore, our results showed that the root microbiome of both species was only weakly influenced by the surrounding water and sediment, with an average of less than 0.7% of ASVs shared. Finally, our study indicates a strong specificity in the bacterial communities of both the phyllosphere and rhizosphere and also raises questions regarding the near absence of Gammaproteobacteria in both the leaves and roots, which remain to be elucidated.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1717288</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1717288</link>
        <title><![CDATA[Understanding the human gut microbiome: from composition to disease association]]></title>
        <pubdate>2026-04-22T00:00:00Z</pubdate>
        <category>Review</category>
        <author>Muneerah Abdullah Alali</author><author>Amal Bakr Shori</author>
        <description><![CDATA[The human gut microbiota is critical for regulating host metabolism, immune responses, epithelial integrity, and systemic homeostasis, and disturbance has been linked to metabolic, inflammatory, and immune-mediated illnesses. Despite significant advances in microbiome research, the interpretation of gut microbiota-disease relationships is still limited by an overreliance on taxonomic profiling and observational study designs, which frequently overlook functional, strain-level, and mechanistic aspects of host-microbiota interactions. Growing research suggests that microbial functional capacity, metabolic activity, and ecological features such as resilience and functional redundancy are better markers of gut health than compositional measurements alone. Nonetheless, significant inter-individual variability, methodological heterogeneity, and dependence on fecal-based analysis continue to limit reproducibility and causal inference across studies. This review integrates current evidence on gut microbiota composition, functional features, and important influencing variables, while emphasizing mechanistic linkages between microbial dysbiosis and major human illnesses, filling significant conceptual gaps in modern microbiome research.]]></description>
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        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1815455</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1815455</link>
        <title><![CDATA[Editorial: The microbiome in cancer therapy response]]></title>
        <pubdate>2026-04-21T00:00:00Z</pubdate>
        <category>Editorial</category>
        <author>Elizabeth M. Park</author><author>Liza Makowski</author><author>Katherine L. Cook</author>
        <description></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1734737</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1734737</link>
        <title><![CDATA[Gut microbiota composition correlates with PBMC microRNA expression following maximal exercise testing in endurance athletes]]></title>
        <pubdate>2026-04-01T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Guy Shalmon</author><author>Guy Shapira</author><author>Rawan Ibrahim</author><author>Ifat Israel-Elgali</author><author>Meitar Grad</author><author>Rani Shlayem</author><author>Ilan Youngster</author><author>Mickey Scheinowitz</author><author>Noam Shomron</author>
        <description><![CDATA[IntroductionMicroRNAs (miRNAs) are key post-transcriptional regulators that also take part in immune responses and recovery processes following exercise. While both gut microbiota composition and peripheral blood mononuclear cell (PBMC)-derived miRNAs are known to be influenced by endurance training, potential correlations between these two systems in athletes remain largely unexplored.ObjectiveThis study aimed to investigate correlations between gut microbiota composition and PBMC miRNA expression following a maximal exercise stress test in endurance athletes.MethodsFifty-eight participants (22 runners, 18 cyclists, and 18 controls) underwent maximal exercise testing, with blood samples collected pre- and post-maximal exercise stress test for small RNA sequencing of PBMCs. Baseline fecal samples were analyzed via 16S rRNA gene sequencing to characterize gut microbiota. Expression data of PBMC miRNAs and microbial taxonomic profiles were integrated to assess potential correlations.ResultsThirteen significant correlations (|r| = 0.41–0.51, p < 0.005) were identified between gut bacterial taxa known to produce short-chain fatty acids (SCFAs)—including Veillonella, Blautia, Coprococcus, Butyrivibrio, Propionibacterium, and Parabacteroides—and the expression of PBMC miRNAs following a maximal exercise test. The significantly expressed PBMC microRNAs included hsa-miR-545-3p, hsa-miR-126-3p, hsa-miR-1292-3p, hsa-miR-6805-5p, hsa-miR-3668, hsa-miR-196b-5p, hsa-miR-602, hsa-miR-324-5p, and hsa-miR-365a-3p, some of which are known to modulate inflammatory pathways and immune cell signaling.ConclusionThis is the first study demonstrating an association between resting gut microbiota composition and PBMC miRNA expression following maximal exercise stress test in endurance athletes. These findings raise the possibility of a complex association between gut microbial composition and PBMC miRNA expression in response to exercise. While causality cannot be inferred, the observed correlations suggest a candidate microbiota–miRNA that warrants further investigation in the context of exercise-induced immune regulation and recovery in athletes.]]></description>
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        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1777385</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1777385</link>
        <title><![CDATA[Gut microbiota and its association with gastrointestinal symptoms and pharmacological treatments in a sibling-matched cohort with autism spectrum disorder]]></title>
        <pubdate>2026-03-24T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Florencia Peñalba</author><author>Andreina Guisande</author><author>Lucía Lamberti</author><author>Camila Rusiñol</author><author>Maite Irastorza</author><author>Florencia Konik</author><author>Claudio Iglesias</author><author>Paula Mendive</author><author>Gabriela Garrido</author><author>Andrés Parada</author><author>Nadia Riera</author>
        <description><![CDATA[Autism Spectrum Disorder (ASD) is a complex neurodevelopmental disorder marked by difficulties in communication, social interaction, and restricted, repetitive behaviors. The gut microbiota has emerged as a key factor in the gut-brain axis relevant to ASD. We conducted a cross-sectional study comparing the gut bacterial composition of children with ASD (n=29) and their neurotypical siblings (NT, n=29). To minimize environmental and lifestyle confounders, all pairs were 4 to 10 years old and cohabiting in the same household in Uruguay. We used full-length 16S rRNA gene (V1–V9) sequencing with the latest R10.4.1 Oxford Nanopore Technologies chemistry, enabling high-resolution microbial characterization. While overall β-diversity did not differ significantly between the ASD and NT groups, we identified specific taxonomic shifts. The ASD group was enriched in taxa like Sellimonas, while the NT group showed enrichment of genera like Faecalibacterium and Coprococcus. Furthermore, we found GI symptoms to be significantly more prevalent in the ASD group and some bacterial genera associated with GI symptomatology. In addition, we explored the association of pharmacological treatments. Antipsychotic use was associated with reduced Akkermansia abundance, whereas melatonin and methylphenidate use were associated with the enrichment of Negativibacillus. This study provides novel insights into the gut microbiome of Uruguayan children with ASD, delineating the influence of GI symptoms and pharmacological load on microbial diversity and composition.]]></description>
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        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1779767</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1779767</link>
        <title><![CDATA[Synergistic analysis of genome-resolved metagenomics and language-based machine learning reveals hidden probiotic genomes in the Indian healthy human gut microbiome]]></title>
        <pubdate>2026-03-18T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Arunmozhi Bharathi Achudhan</author><author>Reetumbhara Parthiban</author><author>Tejaswini Ramasubramanian</author><author>Kanchan Mukesh</author><author>Lilly M. Saleena</author>
        <description><![CDATA[IntroductionThe human gut microbiome plays an essential role in host physiology through metabolic activities such as micronutrient biosynthesis and maintenance of intestinal homeostasis. However, a substantial proportion of gut microorganisms remain uncultured, limiting the characterization of their functional roles and probiotic attributes. Genome-resolved metagenomics enables the recovery of microbial genomes directly from metagenomic data, facilitating the exploration of these uncultivated taxa.MethodsShotgun metagenomic datasets from healthy Indian individuals (n = 110) were analysed using the MuDoGer genome-resolved metagenomic workflow to reconstruct metagenome-assembled genomes (MAGs). MAGs were assessed according to MIMAG quality standards, taxonomically classified, and screened for probiotic characteristics using a machine learning-based prediction classifier. Biosynthetic pathways involved in B-complex vitamins and vitamin K production were identified through comparative genomic analysis.ResultsThe analysis reconstructed 901 MAGs, including 289 high-quality genomes. Taxonomic classification identified 10 bacterial phyla and 109 genera, with Bacillota (47%) and Bacteroidota (41%) dominating the gut microbiome. Probiotic prediction identified 45 candidate probiotic genomes, comprising 22 culturable and 23 unculturable species. The unculturable species Megasphaera sp000417505 (15 MAGs) was the most abundant predicted probiotic taxon. Comparative functional analysis showed a higher abundance of B-vitamin biosynthesis genes in unculturable genomes, whereas culturable genomes contained more vitamin K biosynthesis genes.DiscussionThese findings indicate that both culturable and uncultured gut microorganisms contribute to probiotic-associated functions and micronutrient biosynthesis. Integrating genome-resolved metagenomics with machine learning provides a powerful framework for identifying candidate next-generation probiotics from complex microbial communities.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1691965</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1691965</link>
        <title><![CDATA[Shared taxa but distinct communities: within-individual comparisons of oral, nasal, and urinary microbiomes in asymptomatic “healthy” females]]></title>
        <pubdate>2026-03-09T00:00:00Z</pubdate>
        <category>Brief Research Report</category>
        <author>Sandra Jablonska</author><author>Alex Kula</author><author>Catherine Putonti</author>
        <description><![CDATA[Although microbial community compositions vary throughout the healthy human body, some taxa can be found to reside in multiple anatomical sites. Moreover, some microbiota that have been found to be interconnected, e.g., the gastrointestinal tract and the oral cavity, the female urinary tract and the vagina, the nose (specifically the nares) and the skin. Previously, the urinary microbiome has only been compared to proximal sites; however, several species that inhabit the asymptomatic female urinary tract are also found in distant communities, e.g., Streptococcus mitis, also found in the oral cavity, and Staphylococcus epidermidis, also found in the nares. This prompted our investigation of communities of the oral cavity, nares, and urinary tract, collected from the same individual. In this study, we profiled the oral, nasal, and urinary microbiomes of 26 self-identified “healthy” female participants using 16S rRNA amplicon sequencing. The urinary microbiome was found to be distinct from the oral and nasal communities. Nevertheless, Actinomyces, Corynebacterium, Escherichia + Shigella, Lawsonella, Staphylococcus, and Streptococcus were found to be present within communities of all three anatomical sites. Genera predominant within the oral and nasal communities were often low-abundant taxa within the urinary microbiome. To date, comparisons of the urinary microbiome to microbiomes of other anatomical sites is limited. The distinction between the urinary microbiome and that of the more distant oral and nasal site highlights the role that the environment plays in shaping these communities.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1735358</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1735358</link>
        <title><![CDATA[Maize leaf endosphere microbiome was affected by domestication and shows patterns consistent with microbial dysbiosis]]></title>
        <pubdate>2026-02-23T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Ilksen Topcu</author><author>Julio S. Bernal</author><author>Sanjay Antony-Babu</author>
        <description><![CDATA[BackgroundWhether domestication, post-domestication spread, and breeding affected the maize leaf endosphere microbiota is poorly understood despite the well-known effects of those processes on the crop’s genetics and responses to the environment. We examined the leaf endosphere microbial communities associated with three plant groups (Zea mays): teosintes, maize landraces, and maize elite inbreds. The teosintes group included Balsas (Z. mays parviglumis) and perennial (Zea diploperennis) teosinte, and each maize (Z. mays mays) group included genotypes from Mexico and USA. We used 16S-V4 region amplicon sequencing of the leaf endophytic microbiota to infer how the microbial communities of inbred maize may have been shaped by the crop’s evolution, and whether they were affected by: (i) the transition from a perennial life history to an annual life history in the teosintes; (ii) domestication of maize from Balsas teosinte; (iii) northward spread of landrace maize from Mexico to the US; and (iii) breeding of landrace maizes to produce elite inbreds.ResultsThe leaf endophytic microbial community differed among the plant groups and genotypes, and was affected by domestication, as indicated by a decline in bacterial diversity and changes in microbial community structure between wild (teosinte) and domesticated (maize) Zea. While the microbial community structure was stringent and regulated in the teosintes, it was variable in the maize landraces and inbreds, as evidenced by greater distances to centroid based on Euclidean dissimilarity metric. This pattern was suggestive of microbial dysbiosis in the leaf endosphere associated with domestication and is consistent with predictions of the Anna Karenina principle. This finding marks the first evidence of dysbiosis associated with domestication. FAPROTAX predictions suggested that the teosintes may harbor microbial communities enriched in taxa associated with cellulolytic, chitinolytic, and nitrate respiration functions, while the maizes showed higher fermentation and nitrate reduction functions.ConclusionsOur results showed that the leaf endosphere microbial community structures in maize are consistent with alterations associated with dysbiosis. Our findings enhanced our understanding of the effects of anthropogenic processes including crop domestication, spread, and breeding on the leaf endosphere of elite maize cultivars, and may guide the development of evolutionarily-and ecologically sustainable biofertilizers and biocontrol agents.]]></description>
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