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        <title>Frontiers in Microbiomes | Environmental Microbiomes section | New and Recent Articles</title>
        <link>https://www.frontiersin.org/journals/microbiomes/sections/environmental-microbiomes</link>
        <description>RSS Feed for Environmental Microbiomes section in the Frontiers in Microbiomes journal | New and Recent Articles</description>
        <language>en-us</language>
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        <pubDate>2026-05-12T19:43:40.370+00:00</pubDate>
        <ttl>60</ttl>
        <item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1782119</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1782119</link>
        <title><![CDATA[Uncovering leaf and root microbiomes of mangrove trees in French Guiana]]></title>
        <pubdate>2026-04-22T00:00:00Z</pubdate>
        <category>Brief Research Report</category>
        <author>Mathilde Vigneron</author><author>Sébastien Halary</author><author>Sandrine Crochemore</author><author>Laetitia Plaisance</author><author>Nathalie Parthuisot</author><author>Yvan Bettarel</author>
        <description><![CDATA[Microorganisms are now widely acknowledged as essential contributors to the health and resilience of coastal environments. Yet, mangrove ecosystems, despite offering numerous ecological and economic services, remain relatively overlooked in microbial research. In this study, we examined the bacteriome of the rhizosphere and the phyllosphere of two mangrove tree species: Avicennia germinans and Rhizophora mangle. Both species were sampled along the banks of the Sinnamary estuary in French Guiana. Our results revealed notable differences in microbiome composition between the two organs and between the two tree species. On average, only 0.3% of ASVs were shared between the leaves and roots and 2.2% between A. germinans and R. mangle. The taxonomic differences were characterized mainly by the significant presence of Rhodothermia and Bacteroidia in the leaves and Cyanobacteria and Planctomycetia in the roots. Furthermore, our results showed that the root microbiome of both species was only weakly influenced by the surrounding water and sediment, with an average of less than 0.7% of ASVs shared. Finally, our study indicates a strong specificity in the bacterial communities of both the phyllosphere and rhizosphere and also raises questions regarding the near absence of Gammaproteobacteria in both the leaves and roots, which remain to be elucidated.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1735358</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1735358</link>
        <title><![CDATA[Maize leaf endosphere microbiome was affected by domestication and shows patterns consistent with microbial dysbiosis]]></title>
        <pubdate>2026-02-23T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Ilksen Topcu</author><author>Julio S. Bernal</author><author>Sanjay Antony-Babu</author>
        <description><![CDATA[BackgroundWhether domestication, post-domestication spread, and breeding affected the maize leaf endosphere microbiota is poorly understood despite the well-known effects of those processes on the crop’s genetics and responses to the environment. We examined the leaf endosphere microbial communities associated with three plant groups (Zea mays): teosintes, maize landraces, and maize elite inbreds. The teosintes group included Balsas (Z. mays parviglumis) and perennial (Zea diploperennis) teosinte, and each maize (Z. mays mays) group included genotypes from Mexico and USA. We used 16S-V4 region amplicon sequencing of the leaf endophytic microbiota to infer how the microbial communities of inbred maize may have been shaped by the crop’s evolution, and whether they were affected by: (i) the transition from a perennial life history to an annual life history in the teosintes; (ii) domestication of maize from Balsas teosinte; (iii) northward spread of landrace maize from Mexico to the US; and (iii) breeding of landrace maizes to produce elite inbreds.ResultsThe leaf endophytic microbial community differed among the plant groups and genotypes, and was affected by domestication, as indicated by a decline in bacterial diversity and changes in microbial community structure between wild (teosinte) and domesticated (maize) Zea. While the microbial community structure was stringent and regulated in the teosintes, it was variable in the maize landraces and inbreds, as evidenced by greater distances to centroid based on Euclidean dissimilarity metric. This pattern was suggestive of microbial dysbiosis in the leaf endosphere associated with domestication and is consistent with predictions of the Anna Karenina principle. This finding marks the first evidence of dysbiosis associated with domestication. FAPROTAX predictions suggested that the teosintes may harbor microbial communities enriched in taxa associated with cellulolytic, chitinolytic, and nitrate respiration functions, while the maizes showed higher fermentation and nitrate reduction functions.ConclusionsOur results showed that the leaf endosphere microbial community structures in maize are consistent with alterations associated with dysbiosis. Our findings enhanced our understanding of the effects of anthropogenic processes including crop domestication, spread, and breeding on the leaf endosphere of elite maize cultivars, and may guide the development of evolutionarily-and ecologically sustainable biofertilizers and biocontrol agents.]]></description>
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        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2026.1653994</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2026.1653994</link>
        <title><![CDATA[The prevalence and distribution of Acidobacteriota in the Nama Karoo of South Africa]]></title>
        <pubdate>2026-02-13T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Janca Pieters</author><author>Tersia Andrea Conradie</author><author>Karin Jacobs</author>
        <description><![CDATA[The phylum Acidobacteriota is ubiquitous and a dominant bacterial group in arid lands, playing a crucial role in nutrient cycling and ecosystem functioning. This study explores Acidobacteriota in Southern African arid lands through two complementary approaches. A meta-analysis of 240 soil samples revealed relative abundances ranging from 0.008% to 39.1%, with pH identified as the primary driver of community variance. In addition, 96 bulk soil samples from the Nama Karoo were analyzed using full-length 16S rRNA gene sequencing (V1–V9). Acidobacteriota abundance ranged from 2.3% to 12.2%, with Subdivisions 3, 4, and 6 being the most dominant, while rare subdivisions, such as 2 and 9, showed location-specific distributions. Significant beta-diversity differences (p = 0.002) were linked to soil moisture, electrical conductivity, and nitrate availability, and some subdivisions exhibited correlations with organic carbon and nitrate. Co-occurrence patterns with Planctomycetota and Armatimonadota suggest potential biofilm formation and shared ecological niches. This study provides the first comprehensive assessment of Acidobacteriota in Southern African arid lands, highlighting dominant and rare subdivisions, localized ecological associations, and the need for future work on their metabolic functions and adaptive strategies in arid ecosystems.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1707779</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1707779</link>
        <title><![CDATA[Community assembly following disturbance in batch anaerobic digesters displays highly reproducible secondary succession and a shifting stochastic-deterministic balance]]></title>
        <pubdate>2026-01-27T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Flor de María Guerrero-Toledo</author><author>Teodoro Espinosa-Solares</author><author>Guadalupe Hernández-Eugenio</author><author>David H. Huber</author>
        <description><![CDATA[The great diversity of anaerobic digestion (AD) microbiomes indicates high redundancy and flexibility in the assembly of the community. Moreover, AD microbiomes are frequently subjected to disturbances during start-up and operation that require (re)assembly. We tested the reproducibility of secondary succession and AD community assembly mechanisms using a pre-assembled microbiome that was subjected to intense disturbances. Microbiome diversity and functions were followed in replicate mesophilic batch digesters initiated with multiple stressors, including high feed-to-inoculum ratio and many foreign species. Three 10 L batch digesters were derived from a single long-term CSTR digester pre-adapted to poultry litter feedstock and operated in parallel. Physicochemical parameters (methane, acetate, propionate, butyrate, pH, N-NH3, COD) were measured. Metagenome samples were used to assess diversity and functions. Three performance phases were found along the successional gradient: (1) methane inhibition, (2) high methane production, and (3) low methane plateau. The inventory of species (>1600) remained nearly the same, however the relative abundance of species, families, and functions changed during each successional stage. Syntrophic bacteria peaked in abundance during the mid-succession, high methane stage. Succession of overall KEGG functions was highly similar although species and carbohydrate functions diverged during late succession, suggesting diversity of niche partitioning during degradation of recalcitrant organic matter. We estimated the relative contributions of stochastic and deterministic processes and found a shift in the balance during succession. Early succession was not dominated by either dispersal or selection while late succession was dominated by variable selection. In conclusion, methane production recovered following severe (non-lethal) disturbance in a pre-adapted digester microbiome through a reproducible community assembly pathway that shifted toward deterministic, variable selection over time.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1676639</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1676639</link>
        <title><![CDATA[Multilevel selection theory informs context-dependent mycorrhizal functioning]]></title>
        <pubdate>2026-01-05T00:00:00Z</pubdate>
        <category>Perspective</category>
        <author>Anne M. Katula</author><author>Nancy Collins Johnson</author><author>V. Bala Chaudhary</author><author>Michelle E. Afkhami</author>
        <description><![CDATA[Arbuscular mycorrhizal (AM) fungi form widespread, ancient, and critically important symbioses with plants, but their functioning and beneficial effects are highly context-dependent. This variability stems from eco-evolutionary dynamics operating across multiple levels of biological organization (e.g., genes to holobionts), making generalizable predictions about mycorrhizal outcomes challenging. Multilevel selection theory (MLST), which posits that selection acts simultaneously on multiple levels of biological organization including in opposite directions, can serve as a powerful framework for interpreting this variability in mycorrhizal functional phenotypes. Here, we outline the key principles of MLST and explore how its application to AM fungal symbioses can improve our understanding of this ubiquitous symbiosis. We highlight how four levels of biological organization important to AM symbioses – genes, nuclei, spores, and holobionts – can serve as one or more units of selection under a tripartite framework for the units of selection. We then examine how ecological contexts, such as stress, spatial structure, and community composition, can modulate the balance of selective forces across levels, ultimately shaping the degree of cooperation among symbiotic partners. We conclude by proposing future research directions using MLST to generate deeper insights into the complexity and adaptability of this globally important symbiosis.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1666691</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1666691</link>
        <title><![CDATA[Identification of microbial communities associated with Phymatotrichopsis omnivora sclerotia in two Texas fields]]></title>
        <pubdate>2025-11-28T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Maxwell Sturdivant</author><author>Sanjay Antony-Babu</author><author>Elizabeth Pierson</author><author>Thomas M. Chappell</author><author>Thomas Isakeit</author>
        <description><![CDATA[The soilborne fungus Phymatotrichopsis omnivora causes a mid- to late-season disease known as cotton root rot (CRR). In the United States, P. omnivora is primarily found in Arizona, New Mexico, Oklahoma, and Texas in soils that are alkaline, calcareous, and rarely freeze deeply. This fungus has a wide host range, and can cause substantial losses in cotton crops. In Texas, not all cotton-producing soils have widespread CRR despite having the characteristics to support P. omnivora. Considering the lack of CRR in some Texas soils, we hypothesize that this absence could be due to the microbial composition associated with sclerotia of P. omnivora. The objective of this study was to identify the taxa that make up microbial communities associated with P. omnivora sclerotia in different soils during both the cotton-growing and off seasons. The microbiota associated with P. omnivora sclerotia were identified by burying lab-generated sclerotia in cotton-producing soils. These sclerotia were recovered, along with soil samples for metabarcoding targeting the 16S rRNA gene and the internal transcribed spacer region. When compared to bulk soil, microbial communities associated with sclerotia differed in community composition and taxa relative abundance between a soil with widespread CRR and one in which the disease is absent. Within these soil communities, potential bacterial and fungal biomarkers that reduce CRR were identified. Furthermore, microbial communities of P. omnivora sclerotia changed seasonally. This study presents the first detailed characterization of microorganisms associated with P. omnivora sclerotia in different cotton-producing soils. Our findings support the view that P. omnivora sclerotia serve as ecological hubs, shaping microbial communities with possible implications for disease suppression. Several enriched taxa are culturable, offering candidates for future biocontrol studies that could inform disease management strategies that focus on increased microbial competition.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1675064</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1675064</link>
        <title><![CDATA[Corrosion inhibition of an aluminum alloy by environmentally derived microbial biofilms]]></title>
        <pubdate>2025-11-25T00:00:00Z</pubdate>
        <category>Brief Research Report</category>
        <author>Zachary T. Burton</author><author>Han Liu</author><author>Nathan Stumme</author><author>Scott K. Shaw</author><author>Steven Harris</author><author>Simon Laflamme</author><author>Kaoru Ikuma</author>
        <description><![CDATA[Microbial biofilms can influence corrosion outcomes on metal surfaces. Though past studies have largely focused on microbiologically induced corrosion, we report here that environmentally derived microbial communities can form biofilms that inhibit the corrosion of an aluminum alloy. Our findings point to the importance of complex microbial communities, which are more likely to be found on metals exposed to natural environments, in determining corrosion outcomes and highlight a potential role of microbial interactions in corrosion inhibition.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1709335</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1709335</link>
        <title><![CDATA[Integrated omics analysis of PGPR and AMF effects on soil microbiota and root metabolites in Isatis indigotica]]></title>
        <pubdate>2025-11-17T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Shuai Yang</author><author>Ting Yuan</author><author>Tingting Duan</author><author>Huimin Zhu</author><author>Xiaoxia Zhang</author><author>Haihong Zhang</author><author>Junjie Jiang</author><author>Jun Yang</author><author>Lichuan Hu</author><author>Yu Yu</author><author>Lijun Zhang</author><author>Zhaodi Yuan</author>
        <description><![CDATA[IntroductionThe intensive use of chemical fertilizers and pesticides in modern agriculture has led to severe soil degradation and environmental pollution, which threatens the long-term production of crops. Plant growth-promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF) are promising biofertilizers which can boost plant growth and improve soil quality. However, the combined effects of these factors on medicinal plants such as Isatis indigotica remain unclear.MethodsThis study isolated and identified six plant growth-promoting rhizobacteria (PGPR) strains (Acinetobacter sp. and Bacillus albus) from the rhizosphere of Isatis indigotica. A pot experiment was conducted with control, PGPR inoculation and AMF+PGPR co-inoculation treatments to assess the effects of these treatments on the growth of Isatis indigotica and its soil physicochemical properties. High-throughput sequencing was used to analyse the structure of the rhizosphere microbial community, while non-targeted metabolomics was employed to profile root metabolites. Finally, a redundancy analysis (RDA) was performed to reveal the correlations between the key microbial taxa and the differential metabolites.ResultsAll six of the isolated PGPR strains exhibited multiple capacities that promote plant growth. The pot experiment demonstrated that both PGPR inoculation and AMF+PGPR co-inoculation significantly increased the height and root length of Isatis indigotica compared to the control, while also enhancing the soil’s SOC, TN and AP content. Analysis of the microbial community revealed that the inoculation treatments enriched the rhizosphere microbiome with beneficial taxa such as Proteobacteria and Ascomycota. Metabolomic analysis revealed that inoculation treatments significantly increased the concentrations of key bioactive compounds, such as flavonoids, lipids and amino acids. Furthermore, the RDA revealed a strong correlation between the accumulation of various root metabolites (e.g., benzenesulfonic acids, carbohydrates and fatty acids) and dominant microbial genera (e.g., Acinetobacter, Paenibacillus and Botryotrichum).ConclusionsPGPR and AMF improve the uptake of nutrients and the synthesis of secondary metabolites in Isatis indigotica by altering the structure of the rhizosphere microbiome and root metabolomes. These findings support the use of PGPR and AMF as biofertilizers for sustainably cultivating medicinal plants.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1608732</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1608732</link>
        <title><![CDATA[The EDEN ISS mobile test facility microbiome changes by cleaning and continued use]]></title>
        <pubdate>2025-10-17T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Luis Gaiser</author><author>Kristina Beblo-Vranesevic</author><author>Rob Van Houdt</author><author>Jana Fahrion</author><author>Louise Gillet de Chalonge</author><author>Jess M. Bunchek</author><author>Paul Zabel</author><author>Daniel Schubert</author><author>Petra Rettberg</author>
        <description><![CDATA[IntroductionBioregenerative life support systems (BLSS) utilizing plants and/or microorganisms to provide the crew of a spacecraft with food, clean water, breathable air, and other amenities are likely to form key components of future long-distance spaceflight missions. Extensive testing and validation of such technologies are necessary before they can be implemented. EDEN ISS was a platform in Antarctica that tested various plant cultivation technologies for a BLSS. To ensure the continued operation of a BLSS, it is vital that plants remain healthy, which necessitates the monitoring of the plant production facility microbiome to ensure that pathogens are detected early and countermeasures can be engaged.MethodsSwab surface samples collected in the EDEN ISS Mobile Test Facility (MTF) during different campaigns were used to estimate the bioburden of the various surfaces via viable count. Isolates obtained from the cultivation of the surface samples were identified via partial 16S rRNA gene sequencing. Additionally, 16S amplicon sequencing was performed on DNA extracted directly from the swab samples to characterize the microbiome.Results and discussionThe results revealed that the bioburden of the different sampling positions was not significantly reduced by cleaning, indicating that the employed cleaning regime was unsuited in its current form to adequately lower the bioburden. Identification of the isolates, as well as the full microbiome, revealed mostly environmental genera. However, in both cases, genera containing plant as well as human pathogens, like Pseudomonas and Acinetobacter, were identified and accounted for up to 16.1% of all reads for a sampling condition in the case of Pseudomonas. The two sets of sequencing data had little overlap, with Rhodococcus and Microbacterium being the only genera shared between all sampling conditions and sequencing approaches, and emphasized different aspects of the MTF microbiome, highlighting the advantages of using a combined approach to obtain a more complete picture of the microbiome composition.]]></description>
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        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1655960</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1655960</link>
        <title><![CDATA[Serial nitrogen-phosphate co-limitation controls the primary productivity in the transitional waters of northern South China Sea and the Pearl River Estuary]]></title>
        <pubdate>2025-10-08T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Yuanhao Liu</author><author>Xunying Zhou</author><author>Ruoyu Niu</author><author>Rongman Yan</author><author>Shuaishuai Xu</author><author>Kangli Guo</author><author>Jing Guo</author><author>Jianchang Tao</author><author>Sha Wu</author><author>Shengwei Hou</author>
        <description><![CDATA[Nitrogen (N) and phosphorus (P) are essential nutrients for marine phytoplankton, playing a crucial role in shaping the structure of microbial communities. Nutrients in coastal seawater are influenced by multiple factors, including ocean currents, terrestrial runoff, and anthropogenic activities, leading to region-specific patterns of nutrient limitation. This study investigates nutrient limitation in the transitional waters near Sanmen Island, located at the confluence of the Pearl River Estuary (PRE) and the northern South China Sea. Using 4-hourly in situ time-series observations and nutrient addition experiments, we found that nitrogen limitation persists in this region despite its proximity to the nutrient-rich Pearl River. Urea addition significantly enhanced primary productivity, as evidenced by the increased chlorophyll a concentration and the increased relative abundance of cyanobacteria, whereas phosphate addition alone favored the growth of heterotrophic bacteria, yet limited the growth of cyanobacteria and other primary producers. Combined nitrogen-phosphorus treatments revealed serial co-limitation, where nitrogen relief shifted limitation to phosphorus. In conclusion, these findings highlight the complex nutrient dynamics in transitional coastal waters and underscore the impact of anthropogenic nutrient discharge on ecosystem productivity.]]></description>
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        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1619859</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1619859</link>
        <title><![CDATA[Diverse cultivation strategies are necessary to capture microbial diversity in High Arctic lake sediment]]></title>
        <pubdate>2025-09-26T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Brittany M. Berdy</author><author>Claire E. Williams</author><author>Maria Sizova</author><author>Dawoon Jung</author><author>Nil Tandogan</author><author>Edgar D. Goluch</author><author>Slava Epstein</author>
        <description><![CDATA[While metagenomics has revolutionized our understanding of microbial diversity and function, the cultivation of microorganisms remains indispensable for elucidating their physiological characteristics and potential biotechnological applications. Cultivation provides context to the vast metagenomic datasets and helps verify metagenome-based hypotheses on microbial interactions. The majority of microorganisms remain uncultivated, and this is particularly prominent from extreme environments such as the Arctic. Here we aimed to contribute to the growing body of work investigating microbial ecology in extreme environments by assessing the efficacy of a variety of cultivation approaches in lake sediment in the High Arctic. To try and capture the full breadth of organisms present, we used standard, in situ, and anoxic cultivation methods. We cultured a total of 1,109 microorganisms which clustered into 155 OTUs (97% rRNA gene sequence similarity), representing organisms from Proteobacteria, Actinobacteria, Bacteroidota, and Firmicutes. Importantly, no single method of cultivation proved to be sufficient to represent the cultivable organisms within the environment. Rather, each method resulted in many unique OTUs. Therefore, multiple approaches should be used in conjunction to access the bulk of microbial taxa in a given environment.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1659695</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1659695</link>
        <title><![CDATA[Olivine and dissolved alkalinity trigger different bacterial community shifts in water and oyster gills: insights from a mesocosm experiment]]></title>
        <pubdate>2025-09-26T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Dominik Antoni</author><author>Marco Rump</author><author>Gunnar Gerdts</author>
        <description><![CDATA[Ocean Alkalinity Enhancement (OAE) is a proposed marine carbon dioxide removal strategy that increases seawater buffering capacity and CO2 uptake through the addition of alkaline substances. While OAE shows promise as a climate mitigation tool, its ecological implications remain poorly understood, particularly regarding microbial communities. This paper provides a risk assessment of two different OAE strategies: alkalization with olivine and alkalization with addition of dissolved sodium hydroxide (NaOH). With a mesocosm experiment designed to simulate coastal OAE application, European flat oysters (Ostrea edulis) were chronically exposed to alkalinity-enhanced seawater at two concentrations (250 and 500 µmol·L-¹) derived either from olivine weathering or addition with NaOH. The bacterial community composition of both alkalization types was assessed with amplicon sequencing of the 16S rRNA gene and ecotoxicological impacts were compared to a non-alkalized control. The sampling strategy included samples of the treated waters and the gill microbiome of Ostrea edulis. Our results show that the alkalization type was the primary driver of microbial shifts in the bacterial community of the water samples. Olivine treatments caused distinct taxonomic changes, including an increase in Gammaproteobacteria and Flavobacteriales and a marked decline in Alphaproteobacteria and SAR11 clade. Olivine-treated waters showed reduced richness and evenness. In contrast, dissolved alkalinity treatments produced minimal changes compared to untreated controls. The analysis of the oyster gill microbiome detected a response that was stronger influenced by alkalinity concentration than by alkalization type. Notably, high-alkalinity olivine treatments favored potentially pathogenic Vibrios. Together, these findings highlight that OAE method selection significantly influences bacterial community composition in both marine and host-associated microbiomes. In our experiment, olivine-based OAE posed a greater environmental risk than dissolved OAE. Our study provides insights on the impact of different OAE scenarios, representing a first step toward future field trials and applications.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1607677</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1607677</link>
        <title><![CDATA[Investigating impacts from topsoil stockpile height on soil microbial communities]]></title>
        <pubdate>2025-09-18T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Ashley Fischer</author><author>Jay P. Singh</author><author>Jonathan Van Hamme</author><author>Eric Bottos</author><author>Lauchlan H. Fraser</author>
        <description><![CDATA[Mining activities are often severely disruptive to the landscape, and a significant barrier to reclamation following mining operations is the lack of quality topsoil. This project addresses knowledge gaps in the industry by exploring the compositional nature of topsoil stockpiles and their ability to facilitate post-mining revegetation after long-term storage. To do this, we conducted a microbial profiling of two topsoil stockpiles in the interior of British Columbia, Canada. Both stockpiles show depleted soil quality and significant changes compared to reference soils. Notably, there were declines in microbial diversity and significant shifts in community structure with increasing stockpile depths in one of the stockpiles. These results highlight the influence of topsoil-stockpile height on microbial communities in the soil, which ultimately influences the success of restoration. This research can help the industry to optimize restoration and expedite recovery in their mine-closure practices and provides insights into the general structure of the microbiome existing across a gradient in severely disturbed mining soils.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1614472</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1614472</link>
        <title><![CDATA[Effects of biopolymers, cork, and Rhizobium tropici-derived extracellular polymeric substances on soil microbial communities]]></title>
        <pubdate>2025-08-15T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Alexis K. Craft</author><author>Sowndarya Karapareddy</author><author>Varsha C. Anche</author><author>Madhusudhana R. Janga</author><author>Obaloluwa Soyinka</author><author>Sravan K. Sanathanam</author><author>Seloame T. Nyaku</author><author>Govind C. Sharma</author><author>Zachary Senwo</author><author>Venkateswara R. Sripathi</author>
        <description><![CDATA[IntroductionSoil microorganisms play a crucial role in plant development, while biopolymers, such as cork and Extracellular Polymeric Substances/Exopolysaccharides (EPS), can enhance soil health. However, these amendments may affect DNA extraction and microbial analysis, necessitating the validation of the extraction method before conducting next-generation sequencing (NGS).MethodsThis study evaluated 48 soil samples from Decatur, Alabama (Silt loam) that underwent four treatments: unamended soil (soil.control), soil with cork (soil.cork), soil with EPS (soil.EPS), and soil with both cork and EPS (soil.cork.EPS). Samples were collected at four time intervals (0-, 24-, 48-, and 72-hours post-treatment), with three biological replicates for each treatment. The FastDNA Spin Kit proved the most effective among the six DNA extraction methods tested.Results and discussionAmplicon sequencing of the 16S rRNA gene identified 62,996 amplicon sequence variants (ASVs), with 513 ASVs shared across all time points and 467 ASVs shared among the different treatments. The microbial community was primarily composed of Actinobacteria, Proteobacteria, and Acidobacteria, with Actinobacteria being the most abundant phylum. Actinobacteria, Alphaproteobacteria, Bacilli, and Betaproteobacteria contributed to microbial diversity at the class level. Notable families such as Bacillaceae, Gaiellaceae, Micromonosporaceae, and Streptomycetaceae showed treatment-dependent variations. Core microbiome analysis revealed Bacillus and Gaiella as the dominant genera, which play vital roles in soil ecosystem stability and nutrient cycling. These microbes contribute to carbon sequestration, nitrogen fixation, and phosphorus solubilization, improving soil fertility and plant-microbe interactions. These findings offer valuable insights into microbial dynamics in amended soils, providing information that can improve soil quality and agricultural productivity.]]></description>
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        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1606890</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1606890</link>
        <title><![CDATA[The effect of ocean alkalinity enhancement on pelagic bacterial communities: focus points derived from a mesocosm experiment]]></title>
        <pubdate>2025-08-11T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Dominik Antoni</author><author>Antje Wichels</author><author>Maarten Boersma</author><author>Gunnar Gerdts</author>
        <description><![CDATA[Anthropogenic climate change caused by CO2 emissions forces humanity to reduce the usage of fossil fuels. Along with the task of emission reduction, societies face the task of removing excess CO2 from the atmosphere by using negative emission technologies (NETs). Ocean alkalinity enhancement (OAE) is a proposed NET, aiming at increasing oceanic CO2 uptake through the addition of alkaline substances. This is an anthropogenically accelerated version of rock weathering, a natural global process for atmospheric CO2 regulation. The environmental impacts of OAE remain poorly understood. This study was part of a comprehensive OAE-mesocosm experiment in the North Sea (RETAKE), and focused on the effects of OAE on the pelagic bacterial community during the experiment. We assessed changes in bacterial community structure with 16S rRNA amplicon sequencing and abundance with flow cytometry, to evaluate responses to alkalinity addition. Beta diversity analysis showed that sampling time was the primary driver for community variation, with only marginal structural differences linked to alkalinity treatments. PERMANOVA tests conducted on predictions of functional metabolic pathways of the community revealed significant differences between treatments and baseline controls. A deeper analysis of the identified metabolic pathways revealed little evidence for alkalinity-induced changes. In contrast, total bacterial cell counts were influenced by alkalinity additions, showing delayed abundance peaks at higher concentrations and a non-linear response threshold between 500–750 µmol/L. These dynamics were linked to shifts in chlorophyll concentrations, suggesting an indirect effect of OAE on bacteria mediated by phytoplankton derived resources. This study is one of the first to assess ecological impacts of OAE on bacteria. Our findings highlight a structural resilience of bacterial communities to OAE but also show a quantitative response. By discussing our findings, this study aims to provide focus points, such as a threshold for save levels of alkalinity addition, to direct future research.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1652331</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1652331</link>
        <title><![CDATA[Editorial: Defining a healthy microbiome]]></title>
        <pubdate>2025-07-18T00:00:00Z</pubdate>
        <category>Editorial</category>
        <author>Blaž Stres</author>
        <description></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1608228</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1608228</link>
        <title><![CDATA[Editorial: Interactions between natural and built environment microbiomes in a One Health context]]></title>
        <pubdate>2025-05-07T00:00:00Z</pubdate>
        <category>Editorial</category>
        <author>Weitao Shuai</author><author>Bridget Hegarty</author><author>Alexander Mahnert</author><author>Erica Marie Hartmann</author>
        <description></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1540197</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1540197</link>
        <title><![CDATA[Farm conditions shape microbial communities and their association with methane intensity in dairy cattle: insights from the rumen microbiome at the community level]]></title>
        <pubdate>2025-04-30T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Simon Roques</author><author>Lisanne Koning</author><author>Alex Bossers</author><author>Sanne van Gastelen</author><author>Dirkjan Schokker</author><author>Edoardo Zaccaria</author><author>Léon Šebek</author><author>Soumya K. Kar</author>
        <description><![CDATA[Rumen microbial communities are known to drive methane (CH4) production, but their dynamics in variable “real-world” farming environments are less understood. This research aims to identify specific microbial taxa linked to CH4 emission in commercial dairy farms by employing 16S rRNA gene sequencing, thereby providing a more ecologically relevant understanding of CH4 production in real-world settings.Rumen fluid samples were collected from 212 cows across seventeen Dutch dairy farms. Methane production was measured from these dairy cows using the GreenFeed system and expressed as CH4 intensity (g fat- and protein-corrected milk yield−1). Rumen microbiota was analyzed using 16S rRNA gene amplicon sequencing. Analysis was performed to assess association between microbial taxa and CH4 intensity, using data from individual cattle across the dairy farm. We observed that diet and lactation stage influenced CH4 intensity, consistent with previous studies. Results showed higher CH4 intensity in cows during late lactation, and feeding type, particularly fresh grass intake, strongly influenced rumen microbiota. However, after classifying low and high CH4 emitting cows, only limited differences in microbiota composition could be measured. Few taxa, like Lachnospiraceae, were common across both groups, while Ruminoccocaceae and Rikenellaceae were more abundant in low emitters, and Oscillospiraceae in high emitters. Methanobrevibacter differentiated CH4 emission groups, but archaeal methanogen abundance may not accurately reflect CH4 variation due to methodological limitations, including reliance on relative abundance, limited taxonomic resolution, and primer bias. Using a bacterial-biased 16S rRNA approach, we observed a limited number of consistent bacterial taxa associated with CH4 intensity highlights the challenges of studying dairy farms under practical conditions, where variability in diet, genetics, and management practices complicates the identification of specific rumen microbes associated with CH4 emission. Even with control over key variables, the inherent variability of on-farm conditions impeded the detection of stable microbial patterns. In conclusion, our study clearly indicates that understanding CH4 emissions from dairy cattle in real-world settings fundamentally requires a broader ecological perspective where rumen microbes are recognized as key determinants influencing microbiota signals within multi-factorial farm settings.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1604002</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1604002</link>
        <title><![CDATA[Editorial: Ecology, evolution, and biodiversity of microbiomes and viromes from extreme environments]]></title>
        <pubdate>2025-04-24T00:00:00Z</pubdate>
        <category>Editorial</category>
        <author>Gareth Trubl</author><author>Lucie Malard</author><author>Janina Rahlff</author>
        <description></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/frmbi.2025.1395519</guid>
        <link>https://www.frontiersin.org/articles/10.3389/frmbi.2025.1395519</link>
        <title><![CDATA[Effects of wetting events on mass timber surface microbial communities and VOC emissions: implications for building operation and occupant well-being]]></title>
        <pubdate>2025-04-09T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Gwynne Á. Mhuireach</author><author>Susan Collins</author><author>Leslie Dietz</author><author>Patrick Finn Horve</author><author>Aurélie Laguerre</author><author>Dale Northcutt</author><author>Jason Stenson</author><author>Kevin Van Den Wymelenberg</author><author>Elliott Gall</author><author>Mark Fretz</author>
        <description><![CDATA[IntroductionHumans have used wood as a construction material throughout history. Currently, mass timber products, such as cross-laminated timber (CLT), are becoming more popular as a structural material, since they are renewable and have a lower carbon footprint than concrete or steel. Nonetheless, some building types, such as healthcare, veterinary, and food manufacturing, avoid using structural mass timber due to concerns about microbial growth in the event of wetting. One solution is to use protective coatings on mass timber products to increase moisture resistance, although the coatings themselves may generate concerns about volatile organic compound (VOC) emissions. Natural uncoated wood also produces VOCs, some of which may have intrinsic antimicrobial effects.MethodsIn this study, we inoculated coated and uncoated cross- laminated timber (CLT) blocks with a mock microbial community and isolated each block within individual sealed microcosms. We characterized VOCs and surface microbial communities from the CLT blocks before, during, and after wetting periods of varying durations. VOC concentration and emission rate were analyzed with chromatography-mass spectrometry (GC-MS), while microbial community abundance, diversity, and composition were analyzed through qPCR and shotgun metagenomics.ResultsVOC emissions were elevated immediately after inoculation, then decreased through the remainder of the experiment, except for a plateau during the wetting period. VOCs from uncoated CLT blocks were primarily terpenes, while coated blocks emitted VOCs associated with coatings, plastics, and industrial solvents, as well as terpenes. One VOC—acetoin (3-hydroxy, 2-butanone)—was present at high levels across all samples immediately after microbial inoculation. Bacteria comprised 99.54% of the identified microbial sequences. The plastic control microcosm (not containing a CLT block) had higher abundance of viable bacteria for the majority of the study, but there was no difference in abundance between coated and uncoated blocks. Prior to wetting periods, microbial composition was driven primarily by sampling day, whereas surface type played a larger role during and after wetting periods.]]></description>
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