AUTHOR=Lee Yee Qian , Rajadurai Pathmanathan , Abas Faridah , Othman Iekhsan , Naidu Rakesh 

TITLE=Proteomic Analysis on Anti-Proliferative and Apoptosis Effects of Curcumin Analog, 1,5-bis(4-Hydroxy-3-Methyoxyphenyl)-1,4-Pentadiene-3-One-Treated Human Glioblastoma and Neuroblastoma Cells

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=Volume 8 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2021.645856

DOI=10.3389/fmolb.2021.645856

ISSN=2296-889X

ABSTRACT=Curcumin analogues with excellent biological properties have been synthesized to address and overcome the poor pharmacokinetic profiles of curcumin. This study aims to investigate the cytotoxicity, anti-proliferative and apoptosis-inducing ability of curcumin analogue, MS13 on human glioblastoma U-87 MG and neuroblastoma SH-SY5Y cells, and to examine the global proteome changes in these cells following treatment. Our current findings showed that MS13 induced potent cytotoxicity and anti-proliferative effects on both cells. Increased caspase-3 activity and decreased bcl-2 concentration upon treatment indicate MS13 induces apoptosis in these cells in dose- and time-dependent manner. A label-free shotgun proteomic analysis was performed to reveal global proteome changes in both glioblastoma and neuroblastoma cells and further underpin the underlying anti-cancer mechanism of MS13. Results showed that a total of nine common DEPs, inclusive of GAPDH, ENO1, HSP90AA1, HSP90AB1, EFI5A, HNRNPK, TUBB, H2AFX and SET were identified. Pathway analysis further elucidated that MS13 may induce its anti-tumor effects in both cells via the common enriched pathways, “Glycolysis” and “Post-translational protein modification”. In conclusion, the underlying mechanism of action of MS13 demonstrates potent anti-cancer effects that may indicate potential therapeutic intervention in the management of brain malignancies.