AUTHOR=Li Qi , Chai Lijuan , Dong Gaopan , Zhang Xiaomeng , Du Lupei 

TITLE=NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=Volume 8 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2021.666605

DOI=10.3389/fmolb.2021.666605

ISSN=2296-889X

ABSTRACT=Three environment-sensitive probes were developed for hERG channel based on the nitrobenzoxadiazole (NBD) fluorophore herein. After careful evaluation, probes M1 and M3 have a high affinity for imaging hERG channel in the cell-based experiment. Compared with other fluorescent labeling technologies (such as fluorescent proteins), these probes afford a convenient and economical method to determine hERG channel in vitro and in cellulo. Therefore, these probes are expected to apply for physiological and pathological studies of hERG channels and have the potential to establish a screening system for hERG channels.