AUTHOR=Maniaci Marianna , Boffo Francesca Ludovica , Massignani Enrico , Bonaldi Tiziana 

TITLE=Systematic Analysis of the Impact of R-Methylation on RBPs-RNA Interactions: A Proteomic Approach

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=Volume 8 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2021.688973

DOI=10.3389/fmolb.2021.688973

ISSN=2296-889X

ABSTRACT=RNA binding proteins (RBPs) bind RNAs through specific RNA-binding domains, generating multi-molecular complexes known as ribonucleoproteins (RNPs). Various post-translational modifications (PTMs) have been described to regulate RBP structure, subcellular localization, and interactions with other proteins or RNAs. Recent proteome-wide experiments showed that RBPs are the most representative group within the class of arginine (R)-methylated proteins. Moreover, emerging evidence suggests that this modification plays a role in the regulation of RBP-RNA interaction. Nevertheless, a systematic analysis of how changes in protein-R-methylation can affect globally RBPs-RNA interactions is still missing. 
We describe here a quantitative proteomics approach to profile global changes of RBP-RNA interactions upon the modulation of type I and II protein arginine methyltransferases (PRMT). By coupling the recently described Orthogonal Organic Phase Separation (OOPS) strategy with SILAC labelling and pharmacological modulation of PRMTs, we profile RNA-protein interaction dynamics in dependence of protein-R-methylation. Data are available via ProteomeXchange with identifier PXD024601.