AUTHOR=Li Yamei , Zhao Xueya , He Biwei , Wu Weibin , Zhang Huijuan , Yang Xingyu , Cheng Weiwei 

TITLE=Autophagy Activation by Hypoxia Regulates Angiogenesis and Apoptosis in Oxidized Low-Density Lipoprotein-Induced Preeclampsia

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=Volume 8 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2021.709751

DOI=10.3389/fmolb.2021.709751

ISSN=2296-889X

ABSTRACT=Objective: Autophagy influences a wide range of physiological and pathological processes in human body. In this study, we aimed to investigate the role of autophagy in early-onset preeclampsia (EOPE): autophagy activation by hypoxia could rescue impaired angiogenesis and apoptosis in preeclampsia leading by ox-LDL.
Methods: Transmission electron microscopy was applied to identify autolysosomes in trophoblasts cells of placenta apical region. Quantitative Real-Time Polymerase Chain Reaction, Western blot, Flow cytometry and Wound-healing Assays were adopted to determine autophagy activity, angiogenesis and apoptosis in placenta tissues or HTR8/SVneo cell.
Results: Autophagy activity was inhibited in women placenta that experienced EOPE; autophagy activation by hypoxia enhanced the migration ability, rescued ox-LDL mediated impaired angiogenesis in HTR8/SVneo cells: vascular endothelial growth factor A (VEGFA), downregulation FMS-like tyrosine kinase-1 (FLT1) upregulation; and protected against cell apoptosis (BAX downregulation).
Conclusion: Autophagy could maintain the function of trophoblast cells by differentially regulating the expression of VEGFA and FLT1, protecting against cell apoptosis at the maternal-fetal interface, potentially related to prevention of preeclampsia.