AUTHOR=Bandini Erika , Rossi Tania , Scarpi Emanuela , Gallerani Giulia , Vannini Ivan , Salvi Samanta , Azzali Irene , Melloni Mattia , Salucci Sara , Battistelli Michela , Serra Patrizia , Maltoni Roberta , Cho William C. , Fabbri Francesco 

TITLE=Early Detection and Investigation of Extracellular Vesicles Biomarkers in Breast Cancer

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=Volume 8 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2021.732900

DOI=10.3389/fmolb.2021.732900

ISSN=2296-889X

ABSTRACT=Breast Cancer (BC) is the most diagnosed tumor among women worldwide, and the leading cause of cancer death in female population. The percentage of patients experiencing poor prognosis along with the risk of developing metastasis remains high, also affecting the resistance to current main therapies. Cancer progression and metastatic development are no longer due entirely to their intrinsic characteristics, but are also regulated by signals derived from cells of tumor microenvironment. Extracellular vesicles (EVs) packed with DNA, RNA, and proteins, are the most attractive targets for both therapeutic and diagnostic applications, which represent a decisive challenge as liquid biopsy-based markers. Here we performed a case study based on a multiplexed phenotyping cytofluorimetric approach to characterize BC-derived EVs from BC patients and cell lines, through the detection of multiple antigens. Our data reveal the expression of EVs-related biomarkers derived from BC patient plasma and cell line supernatants, suggesting that EVs could be utilized for characterizing and monitoring disease progression.