AUTHOR=Tang Lin , Yuan Yixiao , Zhai Haoqing , Wang Juan , Zhang Dahang , Liang Huasu , Shi Yulin , Duan Lincan , Jiang Xiulin 

TITLE=MicroRNA-125b-5p Correlates With Prognosis and Lung Adenocarcinoma Progression

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=Volume 8 - 2021

YEAR=2022

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2021.788690

DOI=10.3389/fmolb.2021.788690

ISSN=2296-889X

ABSTRACT=A growing number of studies have focused on investigating microRNAs as crucial regulators in the progression of multiple cancer types. Nevertheless, the biological effects and immunological role of miR-125b-5p in non-small cell lung cancer (NSCLC) have not been determined. The present study aimed to examine the function of miR-125b-5p on the cell proliferation and the outcomes of NSCLC patients. We utilized the diverse public databases analysis the expression, prognosis, diagnostic value and immune role of miR-125b-5p in non-small cell lung cancer. The growth curve, colony formation, flow cytometry, transwell and invasion assays were utilized to determine the function of miR-125b-5p in NSCLC progression. In this study, we found that miR-125b-5p was decreased in NSCLC and correlated with poor prognosis. Pathways analyses revealed that miR-125b-5p was mainly involved in cell proliferation and immune regulation. Moreover, in vitro experiments indicated that over-expression of miR-125b-5p significantly inhibits cell proliferation, migration, invasion and induced cell apoptosis of NSCLC. Finally, we discovered that miR-125b-5p is correlated with immune cell infiltration.  In summary, these results demonstrated that miR-125b-5p serve as a prognostic marker and a therapeutic target for NSCLC.