In the original article, there was a formatting issue in Figure 6 as published. This occurred when the image was converted from a PC generated pdf to an Apple Macintosh generated tif for publication. The corrected Figure 6 appears below.
FIGURE 6
TLS past trans-S-BPDE-dA by T. lanuginosus pols. The ability to bypass BPDE-dA was assayed for (A) polη in thepresence of 4 mM Mg2+, (B) polκ in the presence of 4 mM Mg2+, (C) polη in the presence of 4 mM Mn2+, (D) polκ in the presence of 4 mM Mn2+, (E) polι in the presence of 4 mM Mn2+, and (F) individual, or a mixture of various pols in 4 mM Mn2+. The substrate used in these assays was made by annealing of the 32P labeled primer 5′-CACTGCAGACTCTAAA-3′ and either an undamaged or BPDE-containing template 5′- GCTCGTCAGCAGATTTAGAGTCTGCAGTG-3′, where the underlined bold A stands for the undamaged, or BPDE modified dA. Reactions contained 100 μM each of individual nucleotide (dC, dG, dA, and dT) or a mixture of all four dNTPs as indicated in the figure and were carried out at 37°C for 10 min. Concentrations of enzymes were 0.17 pM for polη, 0.32 pM for polκ, and 0.15 pM for polι. The sequence of the template immediately downstream of the primer (pr) is shown on the left-hand side of each gel pair. The star (*) indicates the position of the adduct.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Statements
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Summary
Keywords
thermostable fungi, Y-family DNA polymerases, phylogenetic analysis, translesion DNA synthesis, DNA polymerase η, DNA polymerase ι, DNA polymerase κ, REV1
Citation
Vaisman A, McDonald JP, Smith MR, Aspelund SL, Evans TC Jr and Woodgate R (2021) Corrigendum: Identification and Characterization of Thermostable Y-Family DNA Polymerases η, ι, κ and Rev1 From a Lower Eukaryote, Thermomyces lanuginosus. Front. Mol. Biosci. 8:819157. doi: 10.3389/fmolb.2021.819157
Received
20 November 2021
Accepted
22 November 2021
Published
15 December 2021
Approved by
Frontiers Editorial Office, Frontiers Media SA, Switzerland
Volume
8 - 2021
Updates
Copyright
© 2021 Vaisman, McDonald, Smith, Aspelund, Evans and Woodgate.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Roger Woodgate, woodgate@nih.gov
§These authors share first authorship
‡ Present address: Sender L. Aspelund, Novavax, Inc., Gaithersburg, MD, United States
ORCID: Alexandra Vaisman orcid.org/0000-0002-2521-1467 John P. McDonald orcid.org/0000-0003-2482-148X Mallory R. Smith orcid.org/0000-0003-1450-7825 Sender L. Aspelund orcid.org/0000-0003-0726-4028 Thomas C. Evans Jr. orcid.org/0000-0001-5406-0146 Roger Woodgate orcid.org/0000-0002-2521-1467
This article was submitted to Structural Biology, a section of the journal Frontiers in Molecular Biosciences
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.