AUTHOR=Gardon Luis , Becker Nina , Rähse Nick , Hölbling Christoph , Apostolidis Athina , Schulz Celina M. , Bochinsky Kevin , Gremer Lothar , Heise Henrike , Lakomek Nils-Alexander 

TITLE=Amyloid fibril formation kinetics of low-pH denatured bovine PI3K-SH3 monitored by three different NMR techniques

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=Volume 10 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2023.1254721

DOI=10.3389/fmolb.2023.1254721

ISSN=2296-889X

ABSTRACT=can be fitted by simple mono-exponential conversion kinetics, with lifetimes τ in the 14 to 38 h range. Atomic force microscopy confirms that fibrils substantially grew in length during the aggregation experiment. This argues for fibril elongation as the dominant growth mechanism in fibril mass (followed by the CP-based solid-state NMR signal).We suggest a combined approach employing both solution NMR and solid-state NMR, back-to-back, on two aliquots of the same sample under seeding conditions as an additional approach to follow monomer depletion and growth of fibril mass simultaneously.