AUTHOR=Oldam Joseph , Tchernyshyov Irina , Van Eyk Jennifer , Troncoso Juan , Glabe Charles G. , Agnetti Giulio 

TITLE=Thioflavin T in-gel staining for ex vivo analysis of cardiac amyloid 

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=Volume 12 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2025.1505250

DOI=10.3389/fmolb.2025.1505250

ISSN=2296-889X

ABSTRACT=There are limited options to quantify and characterize amyloid species from biological samples in a simple manner. Thioflavin T (ThT) has been used for decades to stain amyloid fibrils, but to our knowledge, we were the first to use it in-gel. Thioflavin T in-gel staining is convenient as it is fast, inexpensive, accessible to most laboratories, and compatible with other fluorescent stains and downstream analyses such as mass spectrometry (MS).