AUTHOR=Alsema Astrid M. , Jiang Qiong , Kracht Laura , Gerrits Emma , Dubbelaar Marissa L. , Miedema Anneke , Brouwer Nieske , Hol Elly M. , Middeldorp Jinte , van Dijk Roland , Woodbury Maya , Wachter Astrid , Xi Simon , Möller Thomas , Biber Knut P. , Kooistra Susanne M. , Boddeke Erik W. G. M. , Eggen Bart J. L. 

TITLE=Profiling Microglia From Alzheimer’s Disease Donors and Non-demented Elderly in Acute Human Postmortem Cortical Tissue

JOURNAL=Frontiers in Molecular Neuroscience

VOLUME=Volume 13 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/molecular-neuroscience/articles/10.3389/fnmol.2020.00134

DOI=10.3389/fnmol.2020.00134

ISSN=1662-5099

ABSTRACT=Microglia are the tissue-resident macrophages of the central nervous system (CNS). Recent studies based on bulk and single-cell RNA sequencing in mice indicate high relevance of microglia with respect to risk genes and neuro-inflammation in Alzheimer’s disease (AD). Here, we investigated microglia transcriptomes at bulk and single-cell level in non-demented elderly and AD donors using acute human post-mortem cortical brain samples. We identified 7 human microglial subpopulations with heterogeneity in gene expression. Notably, gene expression profiles and subcluster composition of microglia did not differ between AD donors and non-demented elderly in bulk RNA sequencing nor in single-cell sequencing.