AUTHOR=Chen Bin-Jun , Qian Xiao-qing , Yang Xiao-yu , Jiang Tao , Wang Yan-mei , Lyu Ji-han , Chi Fang-lu , Chen Ping , Ren Dong-dong 

TITLE=Rab11a Regulates the Development of Cilia and Establishment of Planar Cell Polarity in Mammalian Vestibular Hair Cells

JOURNAL=Frontiers in Molecular Neuroscience

VOLUME=Volume 14 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/molecular-neuroscience/articles/10.3389/fnmol.2021.762916

DOI=10.3389/fnmol.2021.762916

ISSN=1662-5099

ABSTRACT=Vestibular organs have unique planar cell polarity, and their normal development and function are dependent on the regular polarity of cilia. requires. Rab11a is a small G protein that participates in the transportation of intracellular and extracellular materials for polarity formation; however, our understanding of the mechanisms of the actions of Rab11a in vestibular organs is limited. Here, we showed that the general shape of the utricle was abnormal in Rab11aCKO/CKO mice. These mice also showed abnormal morphology of the stereocilia bundles, which were reduced in both length and number, as well as disturbed tissue-level polarity. Rab11a affected the distribution of polarity proteins in the vestibular organs, indicating that the normal development of cilia requires Rab11a and intraflagellar transportation. Furthermore, small G protein migration works together with intraflagellar transportation in the normal development of cilia.