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ORIGINAL RESEARCH article
Front. Nanotechnol.
Sec. Biomedical Nanotechnology
Volume 6 - 2024 |
doi: 10.3389/fnano.2024.1419239
This article is part of the Research Topic The Road toward Nano-Based Diagnostics for Health and Disease View all 3 articles
Synthesis of gold nanoparticles coated with glucose oxidase using PVP as passive adsorption linkage
Provisionally accepted- 1 Escuela Superior de Ingeniería Química e Industrias Extractivas, Instituto Politécnico Nacional, Mexico City, México, Mexico
- 2 Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, México City, Mexico
- 3 Universidad Tecnológica de México, Mexico City, México, Mexico
Gold nanoparticles (AuNPs) have great potential as biosensors for glucose detection due to their high sensitivity, as well as their extraordinary physical and chemical properties that improve compatibility with different biorecognition molecules, such as glucose oxidase (GOx). In this work the D-glucose quantification was determined by using the traditional technique based on biochemical reaction of GOx and AuNPs functionalized with polyvinylpirrolidone (PVP) polymer and the enzyme. The AuNPs-PVP-GOx nanocomplexes were characterized by ultraviolet-visible (UV-Visible), Infrared (FTIR), and Raman spectroscopies, as well as scanning electron microscopy (SEM), Z potential, dynamic light scattering (DLS), and thermogravimetry analysis (TGA). In general, these techniques showed significant differences after each functionalization stage with PVP and GOx, for instance it was observed: the presence of different functional groups, an increase of hydrodynamic diameter from 48.60 to 198.77 nm, a shift of the band absorption to larger wavelength, a change in the surface potential and weight loss, and in the morphology of the nanocomplex, which confirm the functionalization. In addition, the enzymatic activity of the AuNPs-PVP-GOx was confirmed through the detection of triiodide ions by UV-Visible spectrophotometry, coming from the oxidation reaction of iodide ions in the presence of H2O2. Furthermore, the nanocomplex synthesized by passive adsorption was evaluated as a possible biosensor for the quantification of D-glucose using a colorimetric assay, obtaining greater sensitivity than the traditional method. These findings indicate that PVP can be used as a linkage medium between AuNPs and GOx, which in turn can be used as a biosensor for the detection of D-glucose at low concentrations in biological fluids.
Keywords: gold nanoparticles, D-glucose, Glucose Oxidase, Polyvinylpyrrolidone, biosensor
Received: 17 Apr 2024; Accepted: 12 Jun 2024.
Copyright: © 2024 Santos-Santos, Zamora-Justo, Vázquez-Martínez, Cabrera-Sierra and Balderas-López. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
José A. Zamora-Justo, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, México City, Mexico
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Guillermo R. Vázquez-Martínez
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