AUTHOR=Khalil Zeinab G. , Sritharan Thulasi , Salim Angela A. , Capon Robert J. 

TITLE=MATRIX: a platform for cultivation profiling and bioactive discovery in natural product research

JOURNAL=Frontiers in Natural Products

VOLUME=Volume 3 - 2024

YEAR=2025

URL=https://www.frontiersin.org/journals/natural-products/articles/10.3389/fntpr.2024.1532543

DOI=10.3389/fntpr.2024.1532543

ISSN=2813-2602

ABSTRACT=The MATRIX protocol presents a novel miniaturized 24-well plate approach for microbial cultivation profiling, enabling the efficient and parallel assessment of microbial metabolite production. This method integrates a media MATRIX within a microbioreactor to facilitate the creation of diverse growth conditions for microbial cultivation. The 24-well format supports the simultaneous study of multiple microbial strains and conditions, allowing the discovery of novel bioactive compounds. By employing a variety of media compositions and cultivations, MATRIX enhances the understanding of microbial metabolic pathways and interactions. This protocol provides a robust platform for natural product discovery, microbial ecology studies, and drug development, showcasing its potential to uncover new natural products and other valuable bioactives. The MATRIX approach offers a resource-efficient platform for microbial biodiscovery, contributing significantly to natural product research and drug development.