Abstract
Under physiological conditions, neuronal network synchronization leads to different oscillatory EEG patterns that are associated with specific behavioral and cognitive functions. Excessive synchronization can, however, lead to focal or generalized epileptiform activities. It is indeed well established that in both epileptic patients and animal models, focal epileptiform EEG patterns are characterized by interictal and ictal (seizure) discharges. Over the last three decades, employing in vitro and in vivo recording techniques, several experimental studies have firmly identified a paradoxical role of GABAA signaling in generating interictal discharges, and in initiating—and perhaps sustaining—focal seizures. Here, we will review these experiments and we will extend our appraisal to evidence suggesting that GABAA signaling may also contribute to epileptogenesis, i.e., the development of plastic changes in brain excitability that leads to the chronic epileptic condition. Overall, we anticipate that this information should provide the rationale for developing new specific pharmacological treatments for patients presenting with focal epileptic disorders such as mesial temporal lobe epilepsy (MTLE).
Background
Neuronal synchronization reflects the integrated activity occurring over time among neuronal networks that are located in the brain (Niedermeyer and da Silva, 2005). Under physiological conditions, neuronal synchronization results in different EEG oscillations that are associated with specific behavioral states, which include cognitive functions and sleep (Steriade et al., 1990; ). However, neuronal synchronization can become abnormally excessive thus leading to focal (; ) and/or generalized epileptic discharges (Timofeev and Steriade, 2004; ). In this review, we will address the cellular and pharmacological mechanisms that cause the generation of epileptiform discharges in in vivo and in vitro animal models of focal epilepsy as well as in epileptic patients who were investigated with invasive electrophysiological recordings (including single unit activity) before undergoing brain surgery. These studies were performed in limbic brain structures—including the hippocampus, the rhinal cortices and the amygdala—since these areas are known to play a role in mesial temporal lobe epilepsy (MTLE) (; ).
Interictal discharges or spikes (i.e., short-lasting events with duration less than 1 s and unaccompanied by any detectable clinical symptom) (Figure 1A) as well as ictal discharges (i.e., periods of abnormal, hypersynchronous activity lasting up to several minutes and thus disrupting normal brain function) (Figure 1B) are recorded in the EEG obtained from animals or patients presenting with a focal epileptic condition such as MTLE (; ; ; ). More recently, it has been shown that focal epileptiform activity is accompanied by the occurrence of high frequency oscillations (HFOs) in the EEG
FIGURE 1
(field potential) recordings (Figure 1C); HFOs are not visible in standard EEG recordings but can be extracted by amplifying the appropriately filtered signals. Based on their frequency content, they have been categorized in two groups: (i) ripples, which include oscillatory events between 80 and 200 Hz and (ii) fast ripples, i.e., oscillatory events occurring between 250 and 500 Hz (,; Staba et al., 2004; ; Urrestarazu et al., 2006; ; ; Lévesque et al., 2011, 2012; Zijlmans et al., 2011). It has been proposed that ripples may represent, mainly, summated IPSPs while fast ripples should mirror synchronized action potential firing generated by principal (glutamatergic) cells (; ), although fast-spiking GABAergic interneurons could also contribute to the generation of fast ripples (). To note how interictal and ictal discharges along with HFOs share some common synchronizing mechanisms.
The topic of our review is the surprisingly active role played by GABAA receptor signaling, in focal epileptiform synchronization. GABAA receptors, once activated, open ionotropic anionic channels that are permeable to Cl– and HCO3– (). Early clinical evidence indicated that interfering with GABA synthesis leads to convulsions (). In addition, experimental studies, which were mainly published in the 1980s, revealed that: (i) several convulsive drugs are GABAA receptor antagonists (; Schwartzkroin and Prince, 1980; ); (ii) inhibition is markedly reduced at the onset of electrographic hippocampal and neocortical seizures (; ); (iii) functional disconnection of interneurons from excitatory inputs causes a decrease in inhibition in epileptic brains (Sloviter, 1987); (iv) inhibition in human MTLE may be reduced due to deficits in GABA transporter functions or alterations in GABAA receptor subunit composition (McDonald et al., 1991; ; Olsen et al., 1992; Williamson et al., 1995). Therefore, in the early 1990s, weakening of inhibition was considered by the majority of epilepsy researchers as the main mechanism leading to focal interictal and ictal discharges and thus to epileptic disorders. This view has been, however, challenged by several successive studies that will be summarized here. To note, however, that we will limit the focus of our review to experimental studies involving electrophysiology methods as it is not meant to cover studies involving other investigative approaches.
GABAA signaling and epileptiform synchronization
Voskuyl and Albus (1985) were the first investigators to report that a pharmacological procedure that does not decrease GABAA receptor function—i.e., bath application of the K+blocker 4-aminopyridine (4AP)—can induce epileptiform activity in isolated rat hippocampal slices. By employing field potential recordings, they identified the spontaneous occurrence of two types of interictal spikes, with distinct shapes and rates of occurrence (Voskuyl and Albus, 1985). These two types of interictal patterns were confirmed to occur in successive studies in which field and intracellular potentials were simultaneously recorded from hippocampal slices (Perreault and Avoli, 1991, 1992). As shown in Figure 2A, field potential recordings obtained during 4AP application revealed: (i) “slow” interictal spikes occurring simultaneously in CA1, CA3, and dentate gyrus (DG), and (ii) “fast” interictal spikes that originate in CA3 and spread to CA1. Moreover, intracellular recordings from CA3 pyramidal cells demonstrated that “slow” interictal spikes were mirrored by slow depolarizations (which were abolished by GABAA receptor antagonists), while “fast” interictal spikes were associated to intracellular bursts of action potentials riding on depolarizations that were caused by ionotropic glutamatergic currents (Figure 2B; Perreault and Avoli, 1991, 1992). It was also confirmed in these experiments (cf., ; Rutecki et al., 1987) that the postsynaptic responses caused by the activation of both GABAA and, presumably, GABAB receptors were not only preserved but greatly increased in amplitude and duration by 4AP (Figure 2C; Perreault and Avoli, 1991); to note as this complex, augmented response was characterized by a pronounced depolarizing component (asterisk in Figure 2C) that may be contributed by HCO3–—an anion that goes through the open GABAA receptor and has an equilibrium potential more positive than Cl– (; )—as well as by the transient increase in extracellular [K+] caused by GABAA receptor postsynaptic activation (cf.). Presumptive ectopic, fractionated action potentials (arrow in Figure 2C) could consistently be recorded during these “slow” stimulus-induced or spontaneous events (), and this evidence has been confirmed in neocortical interneurons as well ().
FIGURE 2
The two types of 4AP-induced interictal spikes were later recorded in extended brain slices—which included the hippocampus proper and other limbic or para-limbic areas such as the entorhinal/perirhinal cortices, the amygdala and the insular cortex (Figure 2D; ,; Sudbury and Avoli, 2007)—as well as in the in vitro guinea pig isolated brain (Figure 2F; Uva et al., 2009). These studies (see also Morris et al., 1996; Lamsa and Kaila, 1997) have demonstrated that “fast” interictal spikes are abolished by ionotropic glutamatergic antagonists, a pharmacological procedure that does not appear to influence the recurrence of “slow” interictal spikes (Figures 2D,F), which are, however, eliminated by application of the GABAA; receptor antagonists picrotoxin (Figure 2E) or bicuculline (Figure 2F) as well as by activating μ-opioid receptors (,); this pharmacological procedure abolishes the presynaptic release of GABA ().
As shown in Figures 2D,F, slow, glutamatergic independent, interictal events continued to propagate through the extended brain slice and in the guinea pig isolated brain. As further discussed below, such propagation may depend on the increases in extracellular [K+] that accompany the slow interictal spikes induced by 4AP. To note as two types of interictal spikes have been identified in in vivo EEG recordings obtained from epileptic animals, and have been thereafter termed “type 1” and “type 2” (; ; Salami et al., 2014; Lévesque et al., 2021b). It should also be emphasized that preservation of inhibition is present in several in vitro models of epileptiform interictal synchronization such as those induced by application of Mg2+ free-medium (Mody et al., 1987; Tancredi et al., 1990), high K+ medium (Rutecki et al., 1985) or tetraethylammonium (Rutecki et al., 1990).
The likely role played by elevations in extracellular [K+] in the spread of the “slow,” mainly GABAergic, interictal spikes recorded during application of 4AP and ionotropic glutamatergic antagonists was originally proposed by Perreault and Avoli (1992). Shortly before, had discovered that GABAA receptor activation, resulting from the application of exogenous GABA or the GABAA receptor agonist THIP, led to increases in extracellular [K+] even when voltage-gated Na+ channels were blocked by tetrodotoxin, thus excluding any relevant contribution of action potential firing to such elevations in extracellular [K+]. As illustrated in Figure 3A, a few years later, Morris et al. (1996) reported that the “slow,” 4AP-induced spikes recorded from different regions of the isolated, adult rat hippocampal slice are mirrored by increases in extracellular [K+] that continue to occur in the presence of the ionotropic glutamate receptor antagonists 6- cyano-7-nitroquinoxalone-2,3-dione (CNQX) and DL-2- amino-5-phosphonovaleric acid (APV); however, these field events—along with their associated increases in extracellular [K+]—were reversibly blocked by the GABAA receptor antagonist bicuculline methiodide (BMI). Similar data have been obtained in successive studies that were aimed at analyzing the elevations in extracellular [K+] associated to the “slow” interictal spikes induced by 4AP in slices of the rat hippocampus (; Lamsa and Kaila, 1997), the rat or mouse entorhinal cortex (; Librizzi et al., 2017) and the human neocortex (Louvel et al., 2001; ). Extracellular [K+] elevations associated to GABAA receptor-mediated spikes were also shown to occur in the entorhinal cortex of the in vitro isolated whole guinea pig brain (Librizzi et al., 2017). Overall, these data indicate that slow interictal spikes induced by 4AP mainly result from synchronous firing of interneurons that causes massive release of GABA, subsequent activation of post-synaptic GABAA receptors and thus sizeable increases in extracellular [K+] through the activation of the KCC2 cotransporter (Viitanen et al., 2010).
FIGURE 3
A turning point on the role played by GABAA receptor signaling in epileptiform synchronization coincided with the discovery that the onset of ictal discharges recorded from juvenile (15–22 day-old) rat hippocampal slices during 4AP application, is shortly preceded, and thus presumably caused by a field event that resemble the “slow” GABAergic spike (asterisk in Figure 3B, 19 day-old field recording) (; , ); to note how this interictal-ictal pattern disappeared with brain maturation to be replaced by a continuous pattern of “fast” and “slow” interictal spikes (Figure 3B, 30 day-old field recording) (cf. also Psarropoulou and Avoli, 1996). However, successive in vitro studies, which were performed in extended brain slices and in the isolated guinea pig brain revealed that ictal (seizure-like) discharges can occur in adult brain tissue during 4AP application as well as that they are initiated and presumably maintained by GABAA receptor signaling (Figure 3C; ,; Sudbury and Avoli, 2007; ; Uva et al., 2013, 2015; Librizzi et al., 2017). The role of GABAA receptor signaling in the initiation of seizure-like activity has been confirmed by computational studies; Kurbatova et al. (2016) have indeed reported that before seizure onset, high frequency firing of GABAergic interneurons generates an increase of the depolarizing GABAA onto pyramidal cells, which induces a massive drop of inhibition that may allow seizure initiation. Interestingly, it has been also shown that the fast activity that occurs at seizure onset and characterizes low-voltage fast onset seizures is associated with interneuron firing, while pyramidal cells remain silent. Employing a biophysically network model (), have also reported, that seizure-like activity triggered by interneuron firing would not depend on depolarizing GABAA signaling, but would instead rely on an increase of intracellular [Cl–], which is sufficient for KCC2 activation, the subsequent accumulation of extracellular [K+] and the development of epileptiform activity ().
In line with the mechanism discussed above (i.e., that interneuron firing leading to GABAA receptor activation does, in turn, cause sizeable elevations in extracellular [K+]), several studies have reported that the initial (sentinel) spikes preceding the ictal events induced by 4AP is associated with interneuron action potential firing along with a large increase in extracellular [K+] (,; Ziburkus et al., 2006; Lévesque et al., 2016; Librizzi et al., 2017). This aspect is further illustrated in Figure 3D. First, double patch-clamp recordings of an interneuron and a principal cell in mouse entorhinal cortex slices demonstrated that interneuron burst discharges coupled with IPSPs (Figure 3Da) or IPSCs (Figure 3Db) in principal neurons occur at the onset of 4AP-induced ictal activity; second, such “pre-ictal” patterns were associated with rises in extracellular [K+] that were closely related to interneuron firing and further enhanced by the ensuing recruitment of neuronal networks into the seizure activity. These results firmly support the view that elevations in extracellular [K+] are caused by interneuron firing, which consistently precedes the initiation of ictal events as well as that these extracellular [K+] elevations contribute to seizure precipitation. Interestingly, the emergence of seizure-like activity during extracellular [K+] perturbations has been demonstrated by a realistic computational model of cortical networks ().
It is well known that elevating extracellular [K+] induces neuronal hyperexcitability along with seizure activity (Zuckermann and Glaser, 1968). Successive studies have demonstrated that increased extracellular [K+] causes a positive shift of the membrane reversal of the GABAA receptor-mediated currents thus weakening inhibition (); it has also been shown that neuronal network resonance, which leads to oscillatory patterns in the beta-gamma range, emerges during increased extracellular [K+] (). These data are therefore in line with the role played by GABAA receptor activation in promoting epileptiform synchronization and thus seizure-like activity. To be emphasized as pharmacological procedures that interfere with GABAA signaling (e.g., GABAA receptor antagonists or μ-opioid receptor agonists) halt ictal discharges induced in vitro by 4AP and replace them with a pattern of recurring, short-lasting interictal spikes (Figure 3E; ,, ; Sudbury and Avoli, 2007). In human epileptic tissue, blockade of GABAA receptors also halts interictal discharges (; ) or modifies their spatial propagation (Sabolek et al., 2012). Suppression of interictal discharges can also be obtained with the application of the NKCC1 blocker bumetanide, in brain slices obtained from pediatric patients with focal cortical dysplasia () or in slices obtained from patients with temporal lobe epilepsy and hippocampal sclerosis (), therefore suggesting that the depolarizing responses to GABA in a subset of pyramidal cells during interictal spikes results from excessively high intracellular [Cl–]. A depolarizing action of GABA due to altered intracellular [Cl–] homeostasis has also been demonstrated in tissue obtained from pediatric patients with cortical dysplasia ().
The paradoxical role played by GABAA receptors in initiating 4AP-induced ictal (seizure-like) events (cf., ) has been confirmed by studies in which optogenetic activation of parvalbumin- or somatostatin-positive interneurons was found capable of triggering ictal events with electrographic features similar to those occurring spontaneously (Shiri et al., 2015, 2016; Yekhlef et al., 2015; Lado et al., 2022). As illustrated in Figure 4A, optogenetic activation of parvalbumin-positive interneurons in the entorhinal cortex (panel b) initiates local ictal discharges that are characterized by an onset that is superimposable to what recorded during spontaneous events (panel a); in fact; the onset of both spontaneous and optogenetic-induced ictal events is typified by one-two interictal-like spikes that lead to fast, beta-gamma oscillations, which characterize the initial component of the seizure activity; these electrographic characteristics represent the hallmark of low-voltage fast onset ictal discharges recorded in patients presenting with focal epileptic disorders (Perucca et al., 2014) and in animal models in vivo (Lévesque et al., 2012). Moreover, it was found in these experiments that optogenetic activation of parvalbumin-positive interneurons could evoke slow interictal spikes both during application of 4AP and after blockade of ionotropic excitatory transmission (Figure 4B; Shiri et al., 2016).
FIGURE 4
The surprisingly active role played by GABAA signaling in initiating and, perhaps, sustaining seizure activity in vitro has been identified under different experimental conditions, including perfusion of low doses of bicuculline in the isolated guinea pig brain (), perfusion of brain slices with Mg2+ free medium (), or high frequency electrical stimuli (Velazquez and Carlen, 1999; ). The contribution of GABAA receptors to epileptiform synchronization is also supported by the ability of CA1 hippocampal networks in vitro to generate prolonged discharges following pharmacological blockade of both GABAB and ionotropic glutamatergic receptors (Uusisaari et al., 2002). Evidence obtained from in vivo models of MTLE have also shown that increased activity of GABA releasing interneurons (which in turns silences principal neurons) coincides with the onset of focal seizures (; ; Toyoda et al., 2015; ). Last but not least, seizure onsets recorded from epileptic patients undergoing presurgical depth electrode investigations, is associated with increased interneuron firing and marked reduction of principal cell excitability (Truccolo et al., 2011; Schevon et al., 2012; ).
GABAA signaling and epileptiform discharges in vivo
The kainic acid (KA) (Lévesque and Avoli, 2013) and the pilocarpine models of MTLE (Lévesque et al., 2021a) have been widely used to study how epileptic discharges are generated from mesial temporal lobe structures in vivo. Both models rely on the chemical induction of an initial brain insult (i.e., a status epilepticus, SE), that is followed a few days later by the development of a chronic epileptic condition. GABAA signaling could play a role in ictogenesis in these animal models, since alterations in GABAA receptor function and in GABA releasing interneurons have been reported (; Schwarzer et al., 1997; Tsunashima et al., 1997; Laurén et al., 2005; ; ; ).
In the KA model, spontaneous seizures occurring in epileptic mice can be stopped, and the frequency of seizures with severe behavioral symptoms reduced, when optogenetic activation of ChR2-expressing PV-positive interneurons is performed in the hippocampus ipsilateral or contralateral to the hippocampus that was injected with KA (). Similar findings were obtained by , who used optogenetics in KA-treated epileptic animals to activate hippocampal PV-interneurons expressing ChRmine; this is a red-shifted opsin that exhibits high sensitivity to light stimulation (Marshel et al., 2019), thus making neurons expressing these opsins sensitive to transcranial optogenetic stimulation. The application of on-demand transcranial optogenetic stimulation to these ChRmine-expressing PV-positive interneurons during the chronic period induced a 51% decrease in seizure duration compared to sham treatment. Interestingly, optogenetic activation of PV-positive interneurons in the hippocampus of KA-treated animals also improves performance in cognitive tasks ().
Such anti-ictogenic effect is not restricted to the hippocampus but it is also observed when optogenetic stimulation is applied to PV-expressing Purkinje cells of the cerebellum, a brain structure that is anatomically and functionally connected to the hippocampus (Watson et al., 2018) and that is known to modulate hippocampal function during cognitive tasks (Zeidler et al., 2020). Krook-Magnuson et al. (2014) found that optogenetic excitation or inhibition of PV-expressing Purkinje cells in the lateral or midline cerebellum of KA-treated animals during the chronic period shortens seizure duration. However, it remains unclear through which mechanisms cerebellar optogenetic stimulation controls hippocampal seizures, since both excitation and inhibition of cerebellar Purkinje cells could decrease seizure duration (Krook-Magnuson et al., 2014). Similar anti-ictogenic effects in the KA model resulting from the activation of GABAergic neuronal populations in remote regions were also reported recently by , who performed optogenetic stimulation of GABAergic populations in the medial septum, a region that sends GABAergic projections to hippocampal GABAergic interneurons (Unal et al., 2015).
In the pilocarpine model, Lévesque et al. (2019) investigated whether continuous, unilateral, optogenetic stimulation of ChR2-expressing PV-positive interneurons in the CA3 subfield of the hippocampus (Figure 5A) could decrease seizure rates in pilocarpine-treated epileptic mice. These results have revealed that activation of PV-ChR2 interneurons at 8 Hz for 30 s every 2 min for 14 continuous days induce a decrease in rates of spontaneous seizures compared to what was observed in PV-Cre (opsin-negative) animals (Figure 5B). Seizure duration (Figure 5C) and proportion of convulsive seizures (Figure 5D) were not decreased by PV optogenetic stimulation; however, rates of interictal spikes (Figure 5E), of interictal spikes with fast ripples (Figure 5F) and of isolated fast ripples (Figure 5G)—which are considered as markers of epileptogenesis ()–were significantly lower in the PV-ChR2 group compared to the PV-Cre group.
FIGURE 5
These findings are in line with the evidence obtained by , who reported a decrease in seizure rates in the KA model by using closed-loop activation of PV-positive interneurons. However, Lévesque et al. (2019) also found that the “residual” seizures that continued to occur, could be triggered by optogenetic stimuli (Figures 5H,I). These data are in line with what was reported in vitro (Shiri et al., 2015, 2016; Yekhlef et al., 2015; ; ).
Concluding remarks
The studies reviewed here disclose an unexpected role played by GABAA receptors in epileptiform synchronization including the generation of interictal and ictal (seizure) events. Such paradoxical role depends on the large increases in extracellular [K+] that are caused by KCC2 activation due to massive release of GABA consequent to synchronous firing of inhibitory interneurons (). We have also summarized recent findings suggesting that activation of inhibitory interneurons can exert unexpected effects on the processes associated to epileptogenesis. These results reveal a complex pattern of participating mechanisms. Thus, while synaptic excitation and voltage-gated Na+ channels remain the key components of synchronous epileptiform discharges, GABAA receptors have emerged as surprising, paradoxical players in the generation of interictal spikes and in the initiation and maintenance of prolonged epileptiform phenomena (i.e., to ictogenesis).
The evidence that enhanced GABAA receptor function supports epileptiform synchronization and thus focal seizure generation may explain the disappointingly limited clinical efficacy of some antiepileptic compounds that were “mechanistically” developed to potentiate GABAA signaling during the 1980s and were introduced into clinical practice at the start of the 1990s. These compounds include γ-vinyl-GABA (which inhibits the breakdown of GABA by the enzyme GABA transaminase) (Rogawski and Löscher, 2004), tiagabine (which increases GABA levels by inhibiting GABA reuptake) (; Pollack et al., 2005), and progabide (Lloyd et al., 1983; Loiseau et al., 1983). It should also be emphasized that benzodiazepines, which can halt seizure activity and stop status epilepticus (Pang and Hirsch, 2005), increase GABAA receptor function by acting on an allosteric “benzodiazepine site” that is located in most of the α subunit-containing GABAA receptors (; ; Olsen, 2015). However, and in line with a synchronizing action of GABA, benzodiazepines have been reported to precipitate seizures, when given intravenously in patients with Lennox-Gastaut syndrome (Perucca et al., 1998).
Statements
Data availability statement
The original contributions presented in this study are included in the article/supplementary material, further inquiries can be directed to the corresponding author.
Author contributions
MA wrote the early draft of this review. All authors contributed to the manuscript revision, read, and approved the submitted version.
Funding
This review was based on experiments that were supported by the Canadian Institutes of Health Research (Grants PJT153310, PJT166178, and MOP130328), CURE and the Savoy Foundation to MA, and the Italian Ministry of Health (Current Research 2021 and Grant RF 2018-12365681), and the Paolo Zorzi Association for Neuroscience (Grant 2021-24 EPICARE project) to MC.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
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Summary
Keywords
epileptiform synchronization, excitatory transmission, GABAA receptor, inhibitory transmission, interictal spikes, mesial temporal lobe epilepsy, seizures
Citation
Avoli M, de Curtis M, Lévesque M, Librizzi L, Uva L and Wang S (2022) GABAA signaling, focal epileptiform synchronization and epileptogenesis. Front. Neural Circuits 16:984802. doi: 10.3389/fncir.2022.984802
Received
02 July 2022
Accepted
13 September 2022
Published
05 October 2022
Volume
16 - 2022
Edited by
Lisa Topolnik, Laval University, Canada
Reviewed by
Sabato Santaniello, University of Connecticut, United States; Carlos Cepeda, University of California, Los Angeles, United States
Updates
Copyright
© 2022 Avoli, de Curtis, Lévesque, Librizzi, Uva and Wang.
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*Correspondence: Massimo Avoli, massimo.avoli@mcgill.ca
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