AUTHOR=Alonso Maria I. , Lamus Francisco , Carnicero Estela , Moro Jose A. , de la Mano Anibal , Fernández Jose M. F. , Desmond Mary E. , Gato Angel 

TITLE=Embryonic Cerebrospinal Fluid Increases Neurogenic Activity in the Brain Ventricular-Subventricular Zone of Adult Mice

JOURNAL=Frontiers in Neuroanatomy

VOLUME=Volume 11 - 2017

YEAR=2017

URL=https://www.frontiersin.org/journals/neuroanatomy/articles/10.3389/fnana.2017.00124

DOI=10.3389/fnana.2017.00124

ISSN=1662-5129

ABSTRACT=29	Neurogenesis  is  a  very  intensive  process  during  early  embryonic  brain  development,
30	becoming dramatically restricted in the adult brain in terms of extension and intensity. We
31	have previously demonstrated the key role of embryonic cerebrospinal fluid in developing
32	brain neurogenic activity. We also showed that cultured adult brain neural stem cells remain
33	competent when responding to the neurogenic influence of embryonic cerebrospinal fluid.
34	However, adult cerebrospinal fluid loses its neurogenic inductive properties. Here, by means
35	of an organotypic culture of adult mouse brain sections, we show that local administration of
36	embryonic cerebrospinal fluid in the subventricular zone niche is able to trigger a neurogenic
37	program in neural stem cells. This leads to a significant increase in the number of non-
38	differentiated neural stem cells, and also in the number of new neurons which show normal
39	migration,	differentiation	and	maturation.	These	new	data	reveal	that	Embryonic
40	Cerebrospinal Fluid activates adult brain neural stem cells, supporting the previous idea that it
41	contains key instructive components which could be useful in adult brain neuroregenerative
42	strategies.