AUTHOR=Laroche Thierry , Burri Olivier , Dubey Lalit Kumar , Seitz Arne 

TITLE=Development of Sample-Adaptable Holders for Lightsheet Microscopy

JOURNAL=Frontiers in Neuroanatomy

VOLUME=Volume 13 - 2019

YEAR=2019

URL=https://www.frontiersin.org/journals/neuroanatomy/articles/10.3389/fnana.2019.00026

DOI=10.3389/fnana.2019.00026

ISSN=1662-5129

ABSTRACT=Multi-user core microscopy facilities are often faced with the challenge to adapt or modify existing instruments. This is essential in order to fulfill the needs of the user community, who need to image a wide range of model organisms; stains and sample thicknesses. In recent years, Lightsheet microscopy has turned into an invaluable tool for both live and cleared sample imaging of many different specimens. This brings new challenges in terms of sample mounting as the classical approach of attachment onto a coverslip cannot be universally applied 
Here we describe the development of a diversified holder which extends the range of samples which can be imaged on a Zeiss Lightsheet microscope Z1. We focus on mounting strategies of cleared specimens; however, the holder and mounting strategy can be applied to live specimens, and different microscopes as well. The proposed methodology provides very high flexibility along with numerous possibility for adaptation based on imaging specimen size, condition and available clearing reagents.